약학회지 (YAKHAK HOEJI)

  • 제54권5호
  • /
  • Pages.377-386
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  • 2010
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  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

단백체 분석을 위한 일차원 및 이차원 역상크로마토그래피의 비교

Comparison of 2-D RP-RP MS/MS with 1-D RP MS/MS for Proteomic Analysis

  • 문평곤 (경북대학교 의과대학 분자의학교실) ;
  • 조영은 (경북대학교 의과대학 분자의학교실) ;
  • 백문창 (경북대학교 의과대학 분자의학교실)
  • Moon, Pyong-Gon (Department of Molecular Medicine, School of Medicine, Kyungpook National University) ;
  • Cho, Young-Eun (Department of Molecular Medicine, School of Medicine, Kyungpook National University) ;
  • Baek, Moon-Chang (Department of Molecular Medicine, School of Medicine, Kyungpook National University)
  • 투고 : 2010.08.17
  • 심사 : 2010.09.19
  • 발행 : 2010.10.31
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초록

Single-dimensional (1-D) and two-dimensional (2-D) LC methods were utilized to separate peptides from various sources followed by MS/MS analysis. Two-dimensional ultra-high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than 1-D LC. The most popular 2-D LC approach used today for proteomic research combines strong cation exchange and reversed-phase LC. We have evaluated an alternative mode for 2-D LC of peptides using 2-D RP-RP nano UPLC Q-TOF Mass Spectrometry, employing reversed-phase columns in both separation dimensions. As control experiments, we identified 129 proteins in 1-D LC and 322 proteins in 2-D LC from E. coli extract peptides. Furthermore, we applied this method to rat primary hepatocyte and a total of 170 proteins were identified from 1-D LC, and 527 proteins were identified from all 2-D LC system. The in-depth protein profiling established by this 2-D LC MS/MS from rat primary hepatocyte could be a very useful reference for future applications in regards to drug induced liver toxicity.

키워드

참고문헌

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