Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The Effect of Xylene and Formaldehyde Inhalation on Testicular Tissue in Rats

  • Gules, O. (Department of Histology-Embryology, Institute of Health Sciences, University of Adnan Menderes) ;
  • Eren, U. (Department of Histology-Embryology, Faculty of Veterinary Medicine, University of Adnan Menderes)
  • Received : 2009.10.11
  • Accepted : 2010.03.31
  • Published : 2010.11.01
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Abstract

In this study, changes in testicular tissues of rats subjected to xylene and formaldehyde inhalation were evaluated. Three experimental groups were included in the study. Each group of rats was exposed to formaldehyde (6 ppm), technical xylene (300 ppm) or a combination of these two agents (150 ppm+3 ppm) for 8 weeks (8 h/d). Control groups were maintained for a period of eight weeks under the same conditions. Staining methods (triple staining, strep ABC method) were applied to examine histometric changes and relaxin like factor (RLF) expression in the testicular tissue. Immunostaining for RLF showed that density of staining for RLF decreased in rats exposed to formaldehyde. Formaldehyde or a combination of formaldehyde and xylene led to a decrease in seminiferous epithelial height. In conclusion, exposure of rats to formaldehyde and xylene-formaldehyde combinations adversely affects Leydig cells (RLF) and seminiferous epithelium of testicular tissue.

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References

  1. Bogatcheva, N. V., A. Truong, S. Feng, W. Engel, I. M. Adham, and A. I. Agoulnik. 2003. GREAT/LGR8 is the only receptor for insulin-like 3 peptide. Mol. Endocrinol. 17(12):2639-2646. https://doi.org/10.1210/me.2003-0096
  2. Bullesbach, E. E. and C. Schwabe. 1999. Tryptophan B27 in the relaxin like factor (RLF) receptor binding. Biochem. 38(10):3073-3078.
  3. Cassidy, S. L., K. M. Dix and T. Jenkins. 1983. Evaluation of a testicular sperm head counting technique using rats exposed to dimethoxyethyl phthalate (DMEP), glycerol-onochlorohydrin (GMCH), epichlorohydrin (ECH), formaldehyde (FA), or methyl methanesulphonate (MMS). Arch. Toxicol. 53:71-78. https://doi.org/10.1007/BF01460003
  4. Chung, W., I. Yu, C. Park, K. Lee, H. Roh and Y. Cha. 1999.Decreased formation of ethoxyacetic acid from ethylene glycol monoethyl eter and reduced atrophy of testes in male rats upon combined administration with toluene and xylene. Toxicol. Lett. 104:143-150. https://doi.org/10.1016/S0378-4274(98)00357-9
  5. Culling, C. F. A., R. T. Allison and W. T. Barr. 1985. Cellular pathology technique. Butterworths and Co Ltd. London.
  6. Fay, M., C. Eisenmann and S. Diwan. 1995. Toxicological profile for xylene. Agency for Toxic Substances and Disease Registry.
  7. Ge, R. S., Q. Dong, C. M. Sottas, H. Chen, B. R. Zikrin and M. P.Hardy. 2005. Gene expression in rat leydig cells during development from the progenitor to adult. Biol. Reprod. 72(6): 1405-1415. https://doi.org/10.1095/biolreprod.104.037499
  8. Golalipour, M. J., R. Azarhoush, S. Ghafari, A. M. Gharravi, S. A.Fazeli and A. Davarian. 2007. Formaldehyde exposure induces histopathological and morphometric changes in the rat testis. Folia Morphol. 66(3):167-171.
  9. Handagama, C. and S. Ariyaratne. 2001. Differentiation of the adult Leydig cell population in the postnatal testis. Biol. Reprod. 65:660-671. https://doi.org/10.1095/biolreprod65.3.660
  10. Hsu, S. M., L. Raine and H. Fanger. 1981. Use of avidin-biotinperoxidase complex (abc) in immunoperoxidase techniques: a comparision between abc and un labeled antibody (pap) procedures. J. Histochem. Cytochem. 29(4):577-580. https://doi.org/10.1177/29.4.6166661
  11. Ivell, R. and R. A. D. Bathgate. 2002. Reproductive biology of the relaxin-like factor (RLF/INSL3). Biol. Reprod. 67:699-705. https://doi.org/10.1095/biolreprod.102.005199
  12. Karimov, K., S. H. N. Dadazhanov and M. S. Gil’dieva. 2003. Rat reproductive cells as biological indicators of the effect of enverionmental factors. Morfologiia 123(1):69-71.
  13. Khan, M. Z., Z. Ali, G. Muhammad, A. Khan and F. Mahmood.2003. Pathological effects of formaldehyde (37% formaldehyde) mixed in feed or administered into the crops of white leghorn cockerels. J. Vet. Med. A. 50(7):354. https://doi.org/10.1046/j.1439-0442.2003.00550.x
  14. Kus, I., I. Zararsiz, M. Ogeturk, H. R. Yilmaz and M. Sarsilmaz.2008. Deneysel formaldehit toksisitesinde testis sod, gsh-px, mda duzeyleri ve $\omega$-3 yag asitlerinin koruyucu etkisi. Fırat Tıp Dergisi. 13(1):01-04.
  15. Lemasters, G. K., D. M. Olsen, J. H. Yiin, J. E. Lockey, R. Shukla,S. G. Selevan, S. M. Schrader, G. P. Toth, D. P. Evenson and G. B. Huszar. 1999. Male reproductive effects of solvent and fuel exposure during aircraft maintenance. Reprod. Toxicol. 13(3):155-156. https://doi.org/10.1016/S0890-6238(99)00012-X
  16. Li, G. L., S. N. Yin, T. Watanabe, H. Nakatsuka, M. Kasahara, H.Abe and M. Ikeda. 1986. Benzene-specific increase in leukocyte alkaline phosphotase activity in rats exposed to vapors of various organic solvents. J. Toxicol. Environ. Health 19(4):581-589. https://doi.org/10.1080/15287398609530954
  17. Monticello, T. M., K. T. Morgan, J. I. Everitt and J. A. Popp. 1989. Effects of formaldehyde gas on the respiratory tract of rhesus monkeys. Am. J. Pathol. 134(3):515-527.
  18. Norback, D., G. Wieslander and C. Edling. 1995. Occupational exposure to volatile organic compounds and other air pollutants from the indoor application of water-based paints. Ann. Occup. Hyg. 39(6):783-794. https://doi.org/10.1093/annhyg/39.6.783
  19. Nylen, P., T. Ebendal, M. Eriksdotter-Nilsson, T. Hansson, A.Henschen, A. C. Johnson, T. Kronevi, U. Kvist, N. O.Sjostrand and G. Hoglund. 1989. Testicular atrophy and loss of nerve growth factor-immunoreactive germ cell line in rats exposed to n-hexane and a protective effect of simultaneous exposure to toluene or xylene. Arch. Toxicol. 63(4):296-307. https://doi.org/10.1007/BF00278643
  20. Orth, J. M. 1982. Proliferation of Sertoli cells in foetal and postnatal rats: a quantitative autoradiographic study. Anat. Rec. 203(4): 485-492. https://doi.org/10.1002/ar.1092030408
  21. Ozen, O. A., M. Yaman, M. Sarsilmaz, A. Songur and I. Kus. 2003. Testicular zinc, copper and iron concentrations in male rats exposed to subacute and subchronic formaldehyde gas inhalation. J. Trace Elem. Med. Biol. 17(1):6. https://doi.org/10.1016/S0946-672X(03)80038-X
  22. Ozen, O. A., N. Akpolat, A. Songur, I. Kus, I. Zararsiz, V. H.Ozcakmak and M. Sarsilmaz. 2005. Effect of formaldehyde inhalation on Hsp70 in seminiferous tubules of rat testes: an immunohistochemical study. Toxicol. Ind. Health 21(9):249-254. https://doi.org/10.1191/0748233705th235oa
  23. Sandikci, M., U. Eren and S. Kum. 2007. Effects of formaldehyde and xylene on alpha-naphthyl acetate esterase positive T lymphocytes in bronchus associated lymphoid tissue and peripheral blood in rats. Rev. Med. 158(6):297-301.
  24. Sadeghian, H., R. Anand-Ivell, M. Balvers, V. Relan and R. Ivell. 2005. Constitutive regulation of the ınsl3 gene in rat leydig cells. Mol. Cell. Endocrinol. 241(1-2):10-20. https://doi.org/10.1016/j.mce.2005.03.017
  25. Shah, B. M., K. D. Vachhrajani, N. J. Chinoy and A. R.Chowdhury. 1987. Formaldehyde induced structural and functional changes in the testis of rats. J. Reprod. Biol. Comp. Endocrinol. 7(1):42-52.
  26. Steinberger E. 1992. Disorder of the male reproductive system. In: Environmental medicine plenum (Ed. A. B. Tarcher). Medical Book Company. Newyork.
  27. Tang, M., Y. Xie, Y. Yi and W. Wang. 2003. Effects of formaldehyde on germ cells of male mice. Wei Sheng Yan Jiu. 32(6):544-548.
  28. Valentine, R. and G. L. Kennedy. 2001. Inhalation toxicology. Chapter 23. In: Principles and methods of toxicology (Ed. A. W. Hayes). Philadelphia, USA: Taylor Francis Publishers:1085-1143.
  29. Yamakada, K. 1993. Influence of lacquer thinner and some organic solvents on reproductive and accessory reproductive organs in the male rat. Biol. Pharm. Bull. 16(4):425-427. https://doi.org/10.1248/bpb.16.425
  30. Yang, Z. W., G. W. Nigel, M. K. David. 1990. A quantitative study of spermatogenesis in the developing rat testis. J. Reprod. Biol. 43: 629-635. https://doi.org/10.1095/biolreprod43.4.629
  31. Zhou, D. X., S. D. Qiu, J. Zhang, H. Tian and H. X. Wang. 2006.The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian J. Androl. 8(5):584-588. https://doi.org/10.1111/j.1745-7262.2006.00198.x

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