Asian-Australasian Journal of Animal Sciences

  • 제23권9호
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  • Pages.1236-1243
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  • 2010
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Inhibitory Activity of Garlic Fermented by Pediococcus pentosaceus KACC 91419 against Antibiotic-resistant Pathogens

  • Ham, Jun-Sang (National Institute of Animal Science, RDA) ;
  • Lee, Seung-Gyu (National Institute of Animal Science, RDA) ;
  • Kim, Min-Kyung (National Institute of Animal Science, RDA) ;
  • Oh, Mi-Hwa (National Institute of Animal Science, RDA) ;
  • Jeong, Seok-Geun (National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hun (National Institute of Animal Science, RDA) ;
  • Lee, Se-Hyung (Department of Animal Resources Science, Dankook University) ;
  • Chae, Jong-Pyo (Department of Animal Resources Science, Dankook University) ;
  • Lee, Ji-Yoon (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Kang, Dae-Kyung (Department of Animal Resources Science, Dankook University)
  • 투고 : 2009.08.31
  • 심사 : 2009.12.30
  • 발행 : 2010.09.01
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초록

The aim of this study was to screen lactic acid bacteria for the fermentation of garlic and to assess the increase in inhibitory activity of garlic fermented against antibiotic-resistant pathogens for use as an animal feed supplement. We screened 45 strains of lactobacillus for the fermentation of garlic. Of these strains, 23 showed similar growth rates with or without allicin. Cultures of the 23 strains were mixed with an equivalent amount of garlic juice and incubated overnight at $37^{\circ}C$. The three strains with the lowest pH values were Lactobacillus paracasei KCTC 3169, L5 strain, and L. reuteri SW. Garlic juice fermented by the L5 strain more strongly inhibited antibiotic-resistant pathogenic bacteria than L. paracasei KCTC 3169, L. reuteri SW, or garlic juice itself. By examining carbohydrate utilization, morphologic properties and 16S rRNA gene sequences, we identified the L5 strain as Pediococcus pentosaceus and deposited it in the name of P. pentosaceus KACC 91419 into the Korea Agricultural Culture Collection. To identify the antimicrobial compound from the garlic filtrate fermented by P. pentosaceus KACC 91419, we fractionated P. pentosaceus KACC 91419 culture on a C18 column and checked the antimicrobial activity of fractions A6 to A10. Only fraction A9 showed inhibitory activity on Staphylococcus aureus. Comparing the mass spectra of the fractions with and without antimicrobial activity, we observed a single dominant product ion (m/z 157.99) from the fraction showing antimicrobial activity. Its molecular mass (157.99) was 2 atomic mass units less than that of allicin (162.02). This suggests that allicin might be converted to its derivative, which has antimicrobial activity, during fermentation by P. pentosaceus KACC 91419.

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