Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Ammonium Production During the Nitrogen-Fixing Process by Wild Paenibacillus Strains and Cell-Free Extract Adsorbed on Nano $TiO_2$ Particles

  • Shokri, Dariush (Faculty of Sciences, Department of Biology, University of Isfahan) ;
  • Emtiazi, Giti (Faculty of Sciences, Department of Biology, University of Isfahan)
  • Received : 2010.03.01
  • Accepted : 2010.05.13
  • Published : 2010.08.28
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Abstract

During the nitrogen-fixing process, ammonia ($NH_3$) is incorporated into glutamate to yield glutamine and is generally not secreted. However, in this study, $NH_3$-excreting strains of nitrogen-fixing Paenibacillus were isolated from soil. The ammonium production by the Paenibacillus strains was assayed in different experiments (dry biomass, wet biomass, cell-free extract, and cell-free extract adsorbed on nano $TiO_2$ particles) inside an innovative bioreactor containing capsules of $N_2$ and $H_2$. In addition, the effects of different $N_2$ and $H_2$ treatments on the formation of $NH_3$ were assayed. The results showed that the dry biomass of the strains produced the most $NH_3$. The dry biomass of the Paenibacillus strain E produced the most $NH_3$ at 1.50, 0.34, and 0.27 ${\mu}M$ $NH_3$/mg biomass/h in the presence of $N_2$ + $H_2$, $N_2$, and $H_2$, respectively, indicating that a combined effluent of $N_2$ and $H_2$ was vital for $NH_3$ production. Notwithstanding, a cell-free extract (CFE) adsorbed on nano $TiO_2$ particles produced the most $NH_3$ and preserved the enzyme activities for a longer period of time, where the $NH_3$ production was 2.45 ${\mu}M$/mg CFE/h over 17 h. Therefore, the present study provides a new, simple, and inexpensive method of $NH_3$ production.

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References

  1. Allen, G. C., D. T. Grimm, and G. H. Elkan. 1991. Oxygen uptake and hydrogen-stimulated nitrogenase activity from Azorhizobium caulinodans ORS571 grown in a succinate-limited chemostat. Appl. Environ. Microb. 57: 3220-3225.
  2. Brewin, B., P. Woodley, and M. Drummond. 1999. The basis of ammonium release in nifL mutants of Azotobacter vinelandii. J. Bacteriol. 181: 7356-7362.
  3. Colnaghi, R., A. Green, L. He, P. Rudnick, and C. Kennedy. 1997. Strategies for increased ammonium production in freeliving or plant associated nitrogen fixing bacteria. Plant Soil 194: 145-154. https://doi.org/10.1023/A:1004268526162
  4. Emerich, D. W., T. Ruiz-Argueso, T. Y. Ching, and H. J. Evans. 1979. Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids. J. Bacteriol. 137: 153-160.
  5. Garrity, G. M., D. G. Brenner, and N. R. Krieg. 2005. Bergey's Manual of Systematic Bacteriology, Vol. 2. Part A, pp. 578-800. Springer.
  6. Habibi, M. H., M. N. Esfahani, and T. A. Egerton. 2007. Photochemical characterization and photocatalytic properties of a nanostructure composite $TiO_2$ film. Int. J. Photoenergy 127: 1-8.
  7. Hanus, F. J., R. J. Maier, and H. J. Evans. 1979. Autotrophic growth of $H_2$-uptake-positive strains of Rhizobium japonicum in an atmosphere supplied with hydrogen gas. Proc. Natl. Acad. Sci. U.S.A. 76: 1788-1792. https://doi.org/10.1073/pnas.76.4.1788
  8. Harrison, J., N. Brugiere, B. Phillipson, S. Ferrario-Mery, T. Becker, A. Limami, and B. Hirel. 2000. Manipulating the pathway of ammonia assimilation through genetic engineering and breeding: Consequences to plant physiology and plant development. Plant Soil 221: 81-93. https://doi.org/10.1023/A:1004715720043
  9. Igarashi, R. Y. and L. C. Seefeldt. 2003. Nitrogen fixation: The mechanism of the Mo-dependent nitrogenase. Crit. Rev. Biochem. Mol. 38: 351-384. https://doi.org/10.1080/10409230391036766
  10. Islam, M. R., Md. Rashedul, M. Madhaiyan, H. P. D. Boruah, W. Yim, G. Lee, et al. 2009. Characterization of plant growthpromoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants. J. Microbiol. Biotechnol. 19: 1213-1222. https://doi.org/10.4014/jmb.0903.03028
  11. Kim, J., H. Jia, C. H. Lee, S. Chungc, J. H. Kwak, Y. Shin, et al. 2006. Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization. Enzyme Microb. Tech. 39: 474-480. https://doi.org/10.1016/j.enzmictec.2005.11.042
  12. Linqiu, C. 2005. Carrier-bound Immobilized Enzymes Principles. Application and Design Edition, Hardcover Handbook/Reference Book, Vol. 35, pp. 1-52. Wiley-VCH, Weinheim.
  13. Narula, N. and K. G. Gupta. 1986. Ammonia excretion by Azotobacter chroococcum in liquid culture and soil in the presence of manganese and clay minerals. Plant Soil 93: 205-209. https://doi.org/10.1007/BF02374222
  14. Rasche, M. E. and D. J. Arp. 1989. Hydrogen inhibition of nitrogen reduction by nitrogenase in isolated soybean nodule bacteroids. Plant Physiol. 91: 663-668. https://doi.org/10.1104/pp.91.2.663
  15. Schubert, K. A. and G. T. Coker. 1981. Ammonia assimilation in Alnus glutinosa and Glycine max. Plant Physiol. 67: 662-665. https://doi.org/10.1104/pp.67.4.662
  16. Shanmugam, K. T. and R. C. Valentine. 1975. Microbial production of ammonium iron from nitrogen. Proc. Natl. Acad. Sci. U.S.A. 72: 136-139. https://doi.org/10.1073/pnas.72.1.136
  17. Singh, S. and K. Lakshminarayana. 1982. Survival and competitive ability of ammonia excreting and nonammonia excreting Azotobacter chroococcum strains in sterile soil. Plant Soil 69: 79-84. https://doi.org/10.1007/BF02185706
  18. Stoltzfus, J. R., R. So, P. P. Malarvithi, J. K. Ladha, and F. J. Bruijn. 1997. Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194: 25-36. https://doi.org/10.1023/A:1004298921641
  19. Troshina, O. Y., L. T. Serebryakova, and P. Lindblad. 1996. Induction of $H_2$-uptake and nitrogenase activities in the Cyanobacterium Anabaena variabilis ATCC 29413: Effects of hydrogen and organic substrate. Curr. Microbiol. 33: 11-15. https://doi.org/10.1007/s002849900066
  20. Wong, T. Y., L. Graham, E. O'hara, and R. J. Maier. 1986. Enrichment for hydrogen-oxidizing Acinetobacter spp. in the rhizosphere of hydrogen-evolving soybean root nodules. Appl. Environ. Microb. 52: 1008-1013.
  21. Yin, C. and F. Da-wei. 1985. Inhibitory effect of molecular hydrogen on $C_{2}H_{2}$-reducing activity in Anabaena 7120 preilluminated in red or blue light. Hydrobiologia 123: 219-221. https://doi.org/10.1007/BF00034382

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