Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Membrane Perturbation Induced by Papiliocin Peptide, Derived from Papilio xuthus, in Candida albicans

  • Lee, June-Young (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Hwang, Jae-Sam (National Academy of Agricultural Science, RDA) ;
  • Hwang, Bo-Mi (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Jin-Kyoung (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Seong-Ryul (National Academy of Agricultural Science, RDA) ;
  • Kim, Yang-Mee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Lee, Dong-Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2010.04.07
  • Accepted : 2010.05.06
  • Published : 2010.08.28
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Abstract

Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin against Candida albicans was investigated. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposomes further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 to 3.3 nm.

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