Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Psacotheasin, a Novel Knottin-Type Antimicrobial Peptide, from Psacothea hilaris

  • Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, June-Young (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Hwang, Bo-Mi (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Nam, Sung-Hee (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Lee, Dong-Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2010.02.02
  • Accepted : 2010.02.25
  • Published : 2010.04.28
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Abstract

We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI were 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.

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References

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