Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Influence of Surfactants on Enhancing Transport of Bacteria in Geological Materials

지질매질체내에서 계면활성제가 박테리아 이동 증진에 미치는 영향

  • Choi, Nag-Choul (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Park, Seong-Jik (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Kim, Song-Bae (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Kim, Dong-Ju (Environmental Hydrogeology Laboratory, Korea University) ;
  • Lee, Seong-Jae (Environmental R&D Center, Halla Energy & Environment)
  • 최낙철 (서울대학교 환경바이오콜로이드공학연구실) ;
  • 박성직 (서울대학교 환경바이오콜로이드공학연구실) ;
  • 김성배 (서울대학교 환경바이오콜로이드공학연구실) ;
  • 김동주 (고려대학교 환경지질연구실) ;
  • 이성재 ((주)한라산업개발 환경기술연구소)
  • Received : 2010.07.23
  • Accepted : 2010.11.17
  • Published : 2010.11.30
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Abstract

This study investigated the effect of surfactants (nonionic surfactant (Tween 20), biosurfactant) on enhancing transport of bacteria (Bacillus subtilis ATCC 6633) in geological materials. Column experiments were performed under various surfactant conditions with columns packed with quartz sand (particle size distribution: 0.5~2.0 mm, mean diameter: 1.0 mm). Bacterial mass recovery, sticking efficiency, and other parameters were quantified from breakthrough curves. Results indicate that bacterial attachment to sand surfaces increased considerably in the presence of mineral salt medium (MSM), especially at the inlet, which was due to the increase of ionic strength by MSM. It was observed that bacterial transport in sand columns was enhanced in the presence of surfactant. Results also show that simultaneous injection of both surfactant and MSM or pre-injection of surfactant was more effective in bacterial transport enhancement than after-injection of surfactant. This study suggests that transport of bacteria in geological materials could be influenced by surfactants and their injection methods.

본 연구에서는 지질매질체내에서 계면활성제(비이온성 계면활성제(Tween 20), 생계면활성제)가 박테리아(Bacillus subtilis ATCC 6633)의 이동 증진에 미치는 영향을 분석하였다. 계면활성제가 존재하는 다양한 실험조건에서 석영모래(입경분포: 0.5~2.0 mm, 평균입경: 1.0 mm)로 충진된 칼럼을 이용하여 박테리아의 이동에 관한 칼럼실험을 수행하였고, 박테리아 파과곡선으로부터 질량회수율과 부착효율 등을 정량화하였다. 실험결과, MSM(박테리아 성장에 필요한 무기물 배지)의 존재 하에서 매질체 표면에 박테리아의 부착이 상당히 증가하였는데(특히, 주입부분에), 이는 MSM으로 인한 이온강도의 증가 때문이다. 계면활성제가 존재할 경우에는, 계면활성제로 인하여 매질체내에서 박테리아 이동이 증진됨을 관찰할 수 있었다. 또한, MSM과 생계면활성제 주입방법에 따른 박테리아의 이동을 비교한 결과, 박테리아의 이동 증진을 위한 주입조건은 박테리아를 함유한 MSM용액보다 계면활성제를 선주입하거나, 또는 MSM용액과 계면활성제를 동시주입하는 것이 효과적임을 알 수 있었다. 본 연구를 통해 계면활성제의 존재와 주입방법이 박테리아의 이동에 영향을 미칠 수 있음을 알 수 있었다.

Keywords

References

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