Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Isolation of a Leucoanthocyanidin Dioxygenase (LDOX) Gene from a Spray-type Chrysanthemum (Dendranthema × grandiflorum) and Its Colored Mutants

스프레이형 국화와 화색변이체로부터 Leucoanthocyanidin dioxygenase (LDOX) 유전자의 분리

  • Chung, Sung-Jin (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Geung-Joo (Department of Horticultural Science, Mokpo National University) ;
  • Lee, Hye-Jung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong-Sub (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 정성진 (한국원자력연구원 정읍방사선과학연구소) ;
  • 이긍주 (목포대학교 원예과학과) ;
  • 이혜정 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김진백 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김동섭 (한국원자력연구원 정읍방사선과학연구소) ;
  • 강시용 (한국원자력연구원 정읍방사선과학연구소)
  • Received : 2010.04.29
  • Accepted : 2010.07.05
  • Published : 2010.10.31
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Abstract

A full-length cDNA and genomic DNA of a $leucoanthocyanidin$ $dioxygenase$ ($DgLDOX$) gene was isolated from the petals of chrysanthemum 'Argus', and comparative features of the gene among three flower color mutants derived from a gamma-ray mutagenesis were characterized. The cDNA coding region of the gene was 1068 bp and was translated into 356 amino acids accordingly. The genomic DNA size was 1346 bp for 'Argus', while three mutants revealed ranges of 1363 to 1374 bp. A single intron between two coding exons for the $DgLDOX$ gene was found, of which size was 112 bp for 'Argus', but 128 or 137 bp for three flower color mutants, indicating that a genomic insertion in the intron occurred during the gamma-ray mutagenesis. DNA blot analysis revealed the $DgLDOX$ gene presenting as a single copy in the chrysanthemum genome. The $DgLDOX$ gene was expressed in both 'Argus' of light-pink color and two purple color mutants (AM1 and AM3) but had very weak expression in only white color mutant (AM2). The results demonstrated that variations in the flower color of the mutants might be associated with changes in the amino acid moieties in the coding exons or fragment insertions in the intron of the $DgLDOX$ gene, which potentially resulted in less expression of the gene in the white colored mutant.

스프레이 국화 'Argus'의 꽃잎으로부터 $DgLDOX$의 전장 cDNA와 genomic DNA를 분리하였고, 감마선 변이원으로부터 유래된 3가지 화색변이체 사이의 다양한 유전자 특성들을 밝혀냈다. cDNA 영역은 1068bp이고 356 amino acid로 변환되었다. Genomic DNA의 크기는 'Argus'에서 1346bp이었고, 3가지 화색 변이체에서는 1363부터 1374의 크기를 나타내었다. $DgLDOX$ 유전자는 두 개의 엑손 사이에 하나의 인트론을 갖고 있는 구조이고, 그 크기는 'Argus'에서 112bp 이지만 3가지 화색 변이체에서는 128 혹은 137bp였다. 이것은 감마선 조사에 의해 인트론 부분에 유전자가 삽입됐다는 것을 나타낸다. DNA 분석 결과 국화의 게놈 내에서는 하나의 $LDOX$ 유전자를 갖는 것이 확인되었다. $DgLDOX$ 유전자의 발현 정도를 분석한 결과, 연분홍의 'Argus'와 두 개의 보라색 변이체(AM1 and AM3) 에서 높게 발현되었으나 흰색 변이체(AM2)에서는 매우 약하게 발현되었다. 이러한 결과들은 $DgLDOX$ 유전자의 인트론에 삽입된 유전자 조각 혹은 엑손 부위의 일부 아미노산의 변화에 의해서 변이체의 화색이 변할 수 있다는 것을 보여주고 있다.

Keywords

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