Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Auxin and Cytokinin Affect Biomass and Bioactive Compound Production from Adventitious Roots of Eleutherococcus koreanum

섬오갈피 부정근 배양 시 오옥신과 사이토키닌이 생장과 생리활성물질 생산에 미치는 영향

  • Lee, Eun-Jung (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Kim, Myong-Ki (Department of Horticulture, Chungbuk National University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • 이은정 (충북대학교 첨단원예기술개발연구센터) ;
  • 김명기 (충북대학교 원예학과) ;
  • 백기엽 (충북대학교 첨단원예기술개발연구센터)
  • Received : 2010.02.16
  • Accepted : 2010.04.12
  • Published : 2010.08.31
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Abstract

In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of $Eleutherococcus$ $koreanum$ in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at $5mg{\cdot}L^{-1}$ indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with $5mg{\cdot}L^{-1}$ IBA compared to ${\alpha}$-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of $5mg{\cdot}L^{-1}$ IBA with $0.1mg{\cdot}L^{-1}$ thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, $N_6$-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of $E.$ $koreanum$. These results suggested that $5mg{\cdot}L^{-1}$ IBA combination with $0.1mg{\cdot}L^{-1}$ TDZ supplementation was most suitable for both biomass and bioactive compound production from adventitious roots of $E.$ $koreanum$.

섬오갈피 부정근의 생장과 생리활성물질 생산에 적합한 MS 배지 내 오옥신(IBA, NAA, IAA)과 사이토키닌(BA, kinetin, TDZ)의 종류와 농도를 구명하고자 250mL 삼각플라스크를 이용하여 5주간 액체 진탕 배양하였다. IBA $5mg{\cdot}L^{-1}$ 처리구에서 생체중과 건물중이 가장 높았으며, 부정근 내 총 페놀과 플라보노이드 함량 역시 NAA나 IAA처리구에 비해 높았다. 오옥신과 사이토키닌의 혼용처리에 따른 부정근의 생장은 IBA $5mg{\cdot}L^{-1}$와 TDZ $0.1mg{\cdot}L^{-1}$와의 혼용 처리구가 IBA 단용 처리구에 비해 생체중과 건물중이 각각 1.4배와 1.6배 증가하여 가장 높은 생장량과 생리활성물질 함량을 나타내었다. 이에 비해 BA와 kinetin과의 혼용 처리는 IBA 단용 처리와 비교해 부정근의 생장과 생리활성물질 생산에 크게 영향을 주지 않았다. 본 실험을 통하여 MS 배지 내 오옥신과 사이토키닌을 IBA $5mg{\cdot}L^{-1}$와 TDZ $0.1mg{\cdot}L^{-1}$로 혼용 처리 할 경우 섬오갈피 부정근의 생장량과 생리활성물질 함량을 크게 증가시킬 수 있음을 확인하였다.

Keywords

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