Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Methanolic Extract of Asterina pectinifera inhibits LPS-Induced Inflammatory Mediators in Murine Macrophage

  • Jo, Wol-Soon (Department of Microbiology, Dong-A University College of Medicine) ;
  • Choi, Yoo-Jin (Department of Microbiology, Dong-A University College of Medicine) ;
  • Kim, Hyoun-Ji (Department of Microbiology, Dong-A University College of Medicine) ;
  • Nam, Byung-Hyouk (Deartment of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Lee, Gye-An (NLP Co. LTD) ;
  • Seo, Su-Yeong (Department of Microbiology, Dong-A University College of Medicine) ;
  • Lee, Sang-Wha (Department of Microbiology, Dong-A University College of Medicine) ;
  • Jeong, Min-Ho (Department of Microbiology, Dong-A University College of Medicine)
  • Published : 2010.03.04
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Abstract

This study aimed to elucidate anti-inflammatory activities from extracts of Asterina pectinifera on nitric oxide (NO) production, TNF-${\alpha}$ and IL-6 release in lipopolysaccharide (LPS)-stimulated murine macrophage cell, RAW264.7. We prepared the methanolic extracts (60-MAP, 70-MAP, 80-MAP and 90-MAP), aqueous extract (W-AP) and functional bioactive compound fraction (He-AP and EA-AP) from Asterina pectinifera according to extract method. The 60-MAP, 70-MAP, 80-MAP, 90-MAP and W-AP were significantly suppressed LPS-induced production NO, TNF-${\alpha}$ and IL-6 secretion in a concentration-dependent manner (P < 0.05). Especially, 80-MAP by extracted 80% methanol had the strongest activity in reduction of inflammatory mediators among these extracts. Indeed, to identify active fraction, which contained potential bioactive compounds, from 80-MAP of Asterina pectinifera, we tested anti-inflammatory activity of the He-AP or the EA-AP. The He-AP was next extracted from 80-MAP and the EA-AP were extracted from the other methanol layer except the He-AP. The EA-AP demonstrated a strong anti-inflammatory effect through its ability to reduce NO production and it also inhibited the production of proinflammatory cytokines such as IL-6 and TNF-${\alpha}$ at low concentration. These results suggested that the methanolic extract from Asterina pectinifera had the potential inhibitory effects on the production of these inflammatory mediators.

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References

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