한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권1호
  • /
  • Pages.23-28
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

한우 혈액에서 PCR을 이용한 Mycobacterium avium ssp paratuberculosis의 검출

Detection of Mycobacterium avium ssp paratuberculosis in Korean Cattle by the Polymerase Chain Reaction

  • 김광현 (전라북도 축산위생연구소) ;
  • 곽길한 (전라북도 축산위생연구소) ;
  • 송희종 (전북대학교 수의학대학 및 생체안전성연구소) ;
  • 조정곤 (전북대학교 수의학대학 및 생체안전성연구소)
  • Kim, Kwang-Hyun (Chonbuk Livestock Development and Research Institute) ;
  • Kwak, Kil-Han (Chonbuk Livestock Development and Research Institute) ;
  • Song, Hee-Jong (College of Veterinary Medicine and Biosafety Research Institute, Chonbuk National University) ;
  • Cho, Jeong-Gon (College of Veterinary Medicine and Biosafety Research Institute, Chonbuk National University)
  • 발행 : 2010.02.28
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초록

Mycobacterium avium ssp paratuberculosis, intracellular bacteria that can cause chronic granulomatous enteritis in cattle, continues to pose significant economic losses and health problem with high prevalence. The purpose of this study is the polymerase chain reaction (PCR)-base strategy for early detection of M. avium ssp paratuberculosis in whole blood. Blood samples were collected from korean cattles in Jeonbuk, Korea. The 16 out of 88 serum samples were detected M. partuberculosis by ELISA. Then samples of infected 8 Korean cattles were amplified by PCR. The PCR amplified targets are 16s rDNA and heat shock protein 65kDa (hsp 65). The 16s rDNA provided a highly sensitive and specific tool for the direct detection of mycobacteria. In addition M. avium was confirmed characteristically by the hsp65. Finally there were sure to M. avium ssp paratuberculosis by IS900 PCR. The restriction fragment length polymorphism was identified by PCR amplifications and subsequence restriction enzyme digestions with Pst I of a hsp65. These results indicate that confirm M. avium with 16s rDNA, hsp65 and a restriction fragment length polymorphism in the hsp65 gene can be seem the other pattern. Therefore, these results can be used for clinical direct detections of M. avium ssp paratuberculosis in whole blood of Korean cattle and also to be used epidemiological researches.

키워드

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