한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권1호
  • /
  • Pages.1-5
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

광학 현미경 및 분자생물학적 방법을 적용한 한우의 Enterocytozoon bieneusi 역학조사

Occurrence of Enterocytozoon bieneusi in Korean Native Cattle Examined by Light Microscopic and Molecular Methods

  • 이존화 (전북대학교 수의과대학) ;
  • 김남수 (충남대학교 수의과대학.동물의과학연구소) ;
  • 전병우 (전북대학교 수의과대학) ;
  • 손화영 (충남대학교 수의과대학.동물의과학연구소) ;
  • 류시윤 (충남대학교 수의과대학.동물의과학연구소) ;
  • 신현진 (충남대학교 수의과대학.동물의과학연구소) ;
  • 박지연 (충남대학교 수의과대학.동물의과학연구소) ;
  • 김현철 (강원대학교 수의과대학) ;
  • 허진 (전북대학교 수의과대학) ;
  • 조정곤 (전북대학교 수의과대학) ;
  • 박배근 (충남대학교 수의과대학.동물의과학연구소)
  • Lee, John-Hwa (College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University) ;
  • Kim, Nam-Soo (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Jeon, Byung-Woo (College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University) ;
  • Son, Hwa-Young (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Ryu, Si-Yun (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Hyun-Jin (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Park, Jie-Yeun (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyeon-Cheol (College of Veterinary Medicine, Kangwon National University) ;
  • Hur, Jin (College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University) ;
  • Cho, Jeong-Gon (College of Veterinary Medicine and Bio-safety Research Institute, Chonbuk National University) ;
  • Park, Bae-Keun (Research Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University)
  • 발행 : 2010.02.28
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초록

미포자충인 Enterocytozoon bieneusi는 최근에 AIDS 환자에 기회감염 되는 병원체로 부각되었다. 이 병원체는 여러 동물에서 발견되고 있으며 인수공통 기생충으로서 특별한 관심의 대상이 되고 있다. 충남, 대전, 전북 지역의 도축장으로부터 특별한 증상을 보이지 않은 건강한 한우 총 1,729 마리의 직장변을 취하여 E. bieneusi의 감염율을 조사하였다. 조사방법은 Trichrome 염색 변법과 중합효소연쇄반응(PCR)을 이용한 분자생물학적 기법을 적용하였다. Trichrome 염색변법을 적용한 광학현미경 관찰에서 194 (11.28%) 개체에서 미포자충이 관찰되었으며, 특히 3월에 가장 높은 감염율을 보였다. 한편 분자생물학적 기법을 적용한 시험에서는 79 (4.59%) 개체에서 양성 반응을 보였으며, 이 역시 3월에 가장 높은 감염율을 나타내었다.

Enterocytozoon bieneusi, a microsporidian species, has emerged as an opportunistic pathogen in AIDS patients. This organism has also been identified in a wide range of animals, and the zoonotic potential of human infections is of particular interest. This study revealed that this organism was found with relatively high prevalence in feces of asymptomatic cattle in Korea. Fecal specimens were obtained from a total of 1,720 cattle in a slaughterhouse located in Chungnam province, Daejeon city and Chonbuk province. After removal of fecal debris by sieving and density gradient centrifugation, samples were examined by microscopic examination and then nested polymerase chain reaction (PCR). Microscopic examination with the modified trichrome staining for the fecal specimens revealed 194 (11.28%) positive calves for microsporidia spore. PCR using the specific primer for E. bieneusi revealed 79 (4.59%) positive calves. The infection ratio of microsporidia was higher in March than other season.

키워드

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