Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Newly Isolated Rhizopus microsporus var. chinensis Capable of Secreting Amyloytic Enzymes with Raw-Starch-Digesting Activity

  • Li, Yu-Na (The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University) ;
  • Shi, Gui-Yang (The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University) ;
  • Wang, Wu (The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University) ;
  • Wang, Zheng-Xiang (The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University)
  • Published : 2010.02.28
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Abstract

A newly isolated active producer of raw-starch-digesting amyloytic enzymes, Rhizopus microsporus var. chinensis CICIM-CU F0088, was screened and identified by morphological characteristics and molecular phylogenetic analyses. This fungus was isolated from the soil of Chinese glue pudding mill, and produced high levels of amylolytic activity under solid-state fermentation with supplementation of starch and wheat bran. Results of thin-layer chromatography showed there are two kinds of amyloytic enzymes formed by this strain, including one $\alpha$-amylase and two glucoamylases. It was found in the electron microscope experiments that the two glucoamylases can digest raw corn starch and have an optimal temperature of $70^{\circ}C$. These results signified that amyloytic enzymes secreted by strain Rhizopus microsporus var. chinensis CICIM-CU F0088 were types of thermostable amyloytic enzymes and able to digest raw corn starch.

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References

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