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Microbial Quality and Safety of Fresh-Cut Broccoli with Different Sanitizers and Contact Times

  • Das, Basanta Kumar (National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Ji-Gang (National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • Published : 2010.02.28

Abstract

This study was conducted to investigate the effects of different sanitizers and contact times on storage quality and microbial growth in fresh-cut broccoli. Fresh broccoli samples were cut into small pieces, washed each for 90 s and 180 s in normal tap water (TW), $100\;{\mu}/l$ chlorinated water (CL, pH 7), electrolyzed water (EW, pH 7.2) containing $100\;{\mu}/l$ free chlorine, or $2\;{\mu}/l$ ozonated water ($O_3$). Then, samples were packaged in 30-${\mu}m$ polyethylene bags and stored at $5^{\circ}C$ for 9 days. No significant differences were observed in gas composition and color parameters ($L^*$, $a^*$, $b^*$, and hue angle) among different sanitizers with contact times. No off-odor was detected during the storage. A longer contact time was not effective in reducing microbial population, except with $O_3$ washing. $O_3$ with 90 s was not much effective in reducing microbial population compared with Cl or EW. However, samples washed with $O_3$ for 180 s observed the lowest numbers of total aerobic and coliform plate counts. The result suggested that, a longer contact time of ozone can be used as a potential sanitizer to maintain the microbial quality and safety of fresh-cut broccoli.

Keywords

References

  1. Allende, A., M. V. Selma, F. Lopez-Galvez, R. Villaescusa, and M. I. Gil. 2008. Role of commercial sanitizers and washing systems on epiphytic microorganisms and sensory quality of fresh-cut escarole and lettuce. Postharvest Biol. Technol. 49: 155-163. https://doi.org/10.1016/j.postharvbio.2007.12.010
  2. Arvind, A. B., A. S. Robert, and A. A. Judith. 2004. Evaluation of wash treatments for survival of foodborne pathogens and maintenance of quality characteristics of fresh-cut apple slices. Food Microbiol. 21: 319-326. https://doi.org/10.1016/j.fm.2003.08.001
  3. Bajji, M., J. Kinet, and S. Lutts. 2002. The use of the electrolyte method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul. 36: 61-70. https://doi.org/10.1023/A:1014732714549
  4. Beltran, D., M. V. Selma, A. Marin, and M. I. Gil. 2005. Ozonated water extends the shelf life of fresh-cut lettuce. J. Agric. Food Chem. 53: 5654-5663. https://doi.org/10.1021/jf050359c
  5. Hassenberg, K., C. Idler, E. Molloy, M. Geyer, M. Plochl, and J. Barnes. 2007. Use of ozone in a lettuce-washing process: An industrial trial. J. Sci. Food Agric. 87: 914-919. https://doi.org/10.1002/jsfa.2815
  6. Hildebrand, P. D., C. F. Forney, J. Song, L. Fan, and K. B. McRae. 2008. Effect of a continuous low ozone exposure (50 nl $l^{-1}$) on decay and quality of stored carrots. Postharvest Biol. Technol. 49: 397-402. https://doi.org/10.1016/j.postharvbio.2008.03.012
  7. Jinhua, D., Y. Fu, and N. Wang. 2009. Effects of aqueous chlorine dioxide treatment on browning of fresh-cut lotus root. LWT Food Sci. Technol. 42: 654-659. https://doi.org/10.1016/j.lwt.2008.08.007
  8. Junhua, H., T. Weiyu, H. Huakun, Z. Bolin, J. Weibo, N. Tiangui, L. Quanhong, and C. Tongyi. 2006 Physiology and quality responses of fresh-cut broccoli florets pretreated with ethanol vapor. J. Food Sci. 71: S385-S389. https://doi.org/10.1111/j.1750-3841.2006.00042.x
  9. Kader, A. A. (ed.). 2002. Postharvest Technology of Horticultural Crops. 3rd Ed. University of California, Division of Agriculture and Natural Resources, Oakland, U.S.A.
  10. Khadre, M. A., A. E. Yousef, and J. G. Kim. 2001. Microbiological aspects of ozone applications in food: A review. J. Food Sci. 66: 1242-1252. https://doi.org/10.1111/j.1365-2621.2001.tb15196.x
  11. Kim, J. G., A. E. Yousef, and G. W. Chism. 1999. Applications of ozone for enhancing the microbiological safety and quality of foods: A review. J. Food Protec. 62: 1071-1087.
  12. Kim, J. G., A. E. Yousef, and M. A. Khadre. 2003. Ozone and its current and future application in the food industry. Adv. Food Nutr. Res. 45: 167-218. https://doi.org/10.1016/S1043-4526(03)45005-5
  13. Kim, J. G., Y. Luo and K. C. Gross. 2004. Effect of package film on the quality of fresh-cut salad savoy. Postharvest Biol. Technol. 32: 99-107. https://doi.org/10.1016/j.postharvbio.2003.10.006
  14. Kim, J. G., Y. Luo, and Y. Tao. 2007. Effect of the sequential treatment of 1-methylcyclopropene and acidified sodium chlorite on microbial growth and quality of fresh-cut cilantro. Postharvest Biol. Technol. 46: 144-149. https://doi.org/10.1016/j.postharvbio.2007.04.011
  15. Kiura, H., K. Sano, S. Morimatsu, T. Nakano, C. Morita, M. Yamaguchi, T. Maeda, and Y. Katsuoka. 2002. Bactericidal activity of electrolyzed acid water from solution containing sodium chloride at low concentration, in comparison with that at high concentration. J. Microbiol. Methods 49: 285-293. https://doi.org/10.1016/S0167-7012(01)00385-2
  16. Koseki, S. and I. Seichiro. 2006. Effect of ozonated water treatment on microbial control and on browning of iceberg lettuce (Lactuca sativa L.). J. Food Protect. 69: 154-160.
  17. Lukasik, J., M. L. Bradley, T. M. Scott, M. Dea, A. Koo, W. Y. Hsu, J. A. Bartz, and S. R. Farrah. 2003. Reduction of poliovirus 1, bacteriophages, Salmonella Montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes. J. Food Protect. 66: 188-193.
  18. Mandelova, L. and J. Totusek. 2007. Broccoli juice treated by high pressure: Chemoprotective effects of sulforaphane and indole-3-carbinol. Int. J. High Pressure Res. 27: 151-156. https://doi.org/10.1080/08957950601082540
  19. Mandrell, R. E., L. Gorski, and M. T. Brandl. 2006. Attachment of microorganisms to fresh produce, pp. 33-73. In G. M. Sapers, J. R. Gorny, and A. E. Yousef (eds.). Microbiology of Fruits and Vegetables. CRC Press, Boca Raton, Florida, U.S.A.
  20. Martínez-Sánchez, A., A. Allende, R. N. Bennett, F. Ferreres, and M. I. Gil. 2006. Microbial, nutritional and sensory quality of rocket leaves as affected by different sanitizers. Postharvest Biol. Technol. 42: 86-97. https://doi.org/10.1016/j.postharvbio.2006.05.010
  21. Muhammad, I. A., S. Junichi, and I. Seiichiro. 2005. Application of electrolyzed water in agriculture and food industries. Food Sci. Technol. Res. 11: 135-150. https://doi.org/10.3136/fstr.11.135
  22. Olmez, H. and M. Y. Akbas. 2009. Optimization of ozone treatment of fresh-cut green leaf lettuce. J. Food Eng. 90: 487-494. https://doi.org/10.1016/j.jfoodeng.2008.07.026
  23. Pascual, A., I. Llorca, and A. Canut. 2007. Use of ozone in food industries for reducing the environmental impact of cleaning and disinfection activities. Trends Food Sci. Technol. 18: S29-S35. https://doi.org/10.1016/j.tifs.2006.10.006
  24. Perez-Balibrea, S., D. A. Moreno, and C. García-Viguera. 2008. Influence of light on health-promoting phytochemicals of broccoli sprouts. J. Sci. Food Agric. 88: 904-910. https://doi.org/10.1002/jsfa.3169
  25. Rico, D., A. B. Martín-Diana, J. M. Barat, and C. Barry-Ryan. 2007. Extending and measuring the quality of fresh-cut fruit and vegetables: A review. Trends Food Sci. Technol. 18: 373-386. https://doi.org/10.1016/j.tifs.2007.03.011
  26. Rico, D., A. B. Martín-Diana, C. Barry-Ryan, J. M. Frias, G. T. M. Henehan, and J. M. Barat. 2008. Use of neutral electrolyzed water for quality maintenance and shelf-life extension of minimally processed lettuce. Innov. Food Sci. Emerging Technol. 9: 37-48. https://doi.org/10.1016/j.ifset.2007.05.002
  27. Sapers, G. M. 2001. Efficacy of washing and sanitizing methods for disinfection of fresh fruit and vegetable products. Food Technol. Biotechnol. 39: 305-311.
  28. Selma, M. V., A. M. Ibañez, A. Allende, M. Cantwell, and T. Suslow. 2008. Effect of gaseous ozone and hot water on microbial and sensory quality of cantaloupe and potential transference of E. coli O157:H7 during cutting. Food Microbiol. 25: 162-168. https://doi.org/10.1016/j.fm.2007.06.003
  29. Serrano, M., D. Martinez-Romero, F. Guillen, S. Castillo, and D. Valero. 2006. Maintenance of broccoli quality and functional properties during cold storage as affected by modified atmosphere packaging. Postharvest Biol. Technol. 39: 61-68. https://doi.org/10.1016/j.postharvbio.2005.08.004
  30. Singh, N., R. K. Singh, A. K. Bhunia, and R. L. Stroshine. 2002. Effect of inoculation and washing methods on the efficacy of different sanitizers against Escherichia coli O157:H7 on lettuce. Food Microbiol. 19: 183-193. https://doi.org/10.1006/fmic.2001.0471
  31. Singh, N., R. K. Singh, A. K. Bhunia, and R. L. Stroshine. 2002. Efficacy of chlorine dioxide, ozone, and thyme essential oil or a sequential washing in killing Escherichia coli O157:H7 on lettuce and baby carrots. LWT Food Sci. Technol. 35: 720-729. https://doi.org/10.1006/fstl.2002.0933
  32. Skog, L. J. and C. L. Chu. 2001. Effect of ozone on qualities of fruits and vegetables in cold storage. Can. J. Plant Sci. 81: 773-778. https://doi.org/10.4141/P00-110
  33. Ukuku, D. O., M. L. Bari, S. Kawamoto, and K. Isshiki. 2005. Use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces. Int. J. Food Microbiol. 104: 225-233. https://doi.org/10.1016/j.ijfoodmicro.2005.01.016
  34. Wang, H., H. Feng, and Y. Luo. 2004. Microbial reduction and storage quality of fresh-cut cilantro washed with acidic electrolyzed water and aqueous ozone. Food Res. Int. 37: 949-956. https://doi.org/10.1016/j.foodres.2004.06.004
  35. Yuk, H.-G., M.-Y. Yoo, J.-W. Yoon, K.-D. Moon, D. L. Marshall, and D.-H. Oh. 2006. Effect of combined ozone and organic acid treatment for control of E. coli O157:H7 and L. monocytogenes on lettuce. J. Food Sci. 71: 83-87.

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