Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Genetically Engineered Pseudomonas fluorescens Strain Possesses Dual Activity Against Phytopathogenic Fungi and Insects

  • Lu, Wenwei (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Zhang, Weiqiong (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Bai, Yan (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Fu, Yingying (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Chen, Jun (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Geng, Xiaolu (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Wang, Yujing (College of Life and Environment Sciences, Shanghai Normal University) ;
  • Xiao, Ming (College of Life and Environment Sciences, Shanghai Normal University)
  • Published : 2010.02.28
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Abstract

A Pseudomonas fluorescens strain was isolated and found to show antagonistic activity against phytopathogenic fungi and to possess a gene responsible for production of antibiotic 2,4-diacetylphloroglucinol. For the extension of biocontrol range, a gene for an Androetonus australis Hector insect toxin 1 (AaHIT1), one of the most known toxic insect-selective peptides, was designed and synthesized according to the preferred codon usage of Pseudomonas fluorescens, cloned, and transformed into the strain by pSUP106 vector, a broad-host-range plasmid. Bioassays indicated that the engineered strain was able to produce AaHIT1 with insecticidal activity, and at the same time retain the activity against plant pathogen. The experiments for nonplanted soil and rhizosphere colonization showed that, similar to the population of the wild-type strain, that of the engineered strain remained relatively constant in the first 10 days, and the subsequent 50 days, suggesting that AaHIT1 expression in the bacterial cell does not substantially impair its long-term colonization. It is first reported that a Pseudomonas fluorescens strain expressing an active scorpion neurotoxin has dual activity against phytopathogenic fungi and insects, making at attractive for agronomic applications.

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References

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