KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of Thermostable Fusant, CHY1612 for Lignocellulosic Simultaneous Saccharification and Fermentation

섬유질계 동시당화발효를 위한 내열성 융합 효모, Kluyveromyces marxianus CHY1612의 개발

  • Kang, Hyun-Woo (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd.) ;
  • Kim, Yule (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd.) ;
  • Park, Ju-Yong (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Min, Ji-Ho (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Choi, Gi-Wook (Changhae Institute of Cassava and Ethanol Research, Changhae Ethanol Co., Ltd.)
  • Received : 2010.11.30
  • Accepted : 2010.12.22
  • Published : 2010.12.31
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Abstract

To develop thermostable ethanol fermentative yeast strain for lignocellulosic simultaneous saccharification and fermentation, high ethanol producing yeast, Saccharomyces cerevisiae CHY1012 and thermostable yeast, Kluyveromyces marxianus CHY1703 were fused by protoplast fusion. The thermostable fusant, CHY1612 was identified as a Kluyveromyces marxianus by phenotypic and physiological characteristics, as well as molecular analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1 + 2 regions. For lignocellulosic ethanol production, AFEX pretreated barley straw at $150^{\circ}C$ for 90 min was used in a simultaneous saccharification and fermentation (SSF) process using thermotolerant CHY1612. The SSF from 16% pretreated barley straw at $43^{\circ}C$ gave a saccharification ratio of 90.5%, a final ethanol concentration of 38.5 g/L, and a theoretical yield of 91.2%. These results show that K. marxianus CHY1612 has potential for lignocellulosic ethanol production through simultaneous saccharification and fermentation with further development of process.

Keywords

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