KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of Pigment-Producing Kocuria sp. K70 and the Optimal Conditions for Pigment Production and Physical Stability

색소생성 균주 Kocuria sp. K70의 특징과 색소생성 최적 조건 및 물리적 안정성

  • Received : 2010.11.24
  • Accepted : 2010.12.25
  • Published : 2010.12.31
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Abstract

Marine bacterium producing pigment was isolated from the solar saltern of Mijo-myeon, Namhae, Korea. Based on phenotypic characteristics and 16S rRNA sequence analysis, the strain was identified as Kocuria sp., which produced a yellow pigment. The pigment showed UV absorption maximum at 469nm. The bacterial strain grew well on Marine broth 2216 culture medium. Productivity of the pigment reached the maximum value after 44 hours at $30^{\circ}C$, 2% NaCl and pH 6.0. The pigment was produced best when supplied by 1% lactose as a carbon source and 1% beef extract as a nitrogen source. The result of the color stability study showed that pigment extracted from the strain by ethanol was stable at $-20-25^{\circ}C$ and also showed higher stability over 70% for 14 days in light conditions at $25^{\circ}C$. The pigment extract was also stable for all metal ions tested, except for $FeCl_2$.

Keywords

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