The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Simultaneous Determination of Albiflorin, Cinnamaldehyde, Cinnamic Acid, Daidzin, Glycyrrhizin, Liquiritin, Paeoniflorin and Puerarin in Galgeun-tang by HPLC-PDA

  • Seo, Chang-Seob (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • Received : 2010.08.17
  • Accepted : 2010.11.01
  • Published : 2010.11.30
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Abstract

Objectives: We investigated to develop and validate HPLC-PDA methods for simultaneous determination of eight constituents in Galgeun-tang (GGT). Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 230 nm, 254 nm, and 280 nm, were used for quantification of the eight marker components of GGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results: Calibration curves were acquired with $r^2$ > 0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each component was in the range of 87.33-101.38%, with an RSD less than 7.0%. The contents of the eight components in GGT were 1.98-12.17 mg/g. Conclusions: The established method will be applied for the quantification of marker components in GGT.

Keywords

References

  1. Xue TH, Roy R. Studying traditional Chinese medicine. Science. 2003; 300:740-1. https://doi.org/10.1126/science.300.5620.740
  2. Normile D. Asian medicine: The new face of traditional Chinese medicine. Science. 2003; 299:188-90. https://doi.org/10.1126/science.299.5604.188
  3. Jiang WY. Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science. Trends Pharmacol Sci. 2005; 26:558-63. https://doi.org/10.1016/j.tips.2005.09.006
  4. Liu S, Yi LZ, Liang YZ. Traditional Chinese medicine and separation science. J Sep Sci. 2008; 31:2113-37. https://doi.org/10.1002/jssc.200800134
  5. Won JB, Ma JY, Ma CJ. Simultaneous determination of four marker components in yukmijihwang-tang by high performance liquid chromatography/diode array detector. Arch Pharm Res. 2010; 33:619-23. https://doi.org/10.1007/s12272-010-0417-9
  6. Hur J. Dong-eui-bo-gam. Seoul: Namsandang. 1994:390.
  7. Chai IS. Sang Han Ron Yeok Jeong. Seoul: Gomoonsa. 2000:329.
  8. Park HJ. Kum Kue Yo Ryak. Seoul: Seowondang. 1978:83.
  9. Tang W, Eisenbrand G. Chinese drugs of plant origin-chemistry, pharmacology and use in traditional and modern medicine. New York: Springer Berlin, Heidelberg. 1992:797-803.
  10. Zhu YP. Chinese materia medica-chemistry, pharmacology and applications. Amsterdam: Harwood Academic Publishers. 1998:92-6.
  11. Kim DR, Kwak GS, Jeong SM, Lee SC, Ha JU. Comparison of the antioxidative abilities of commercial Galgeuntang. J Korean Soc Food Sci Nutr. 2003; 32:728-32. https://doi.org/10.3746/jkfn.2003.32.5.728
  12. Shin JM, Kim YO, Baek SH. Free radical scavenging activity and kinetic behavior of the galgeuntang water extract. Ori Pharm Exp Med. 2008; 8:32-8. https://doi.org/10.3742/OPEM.2008.8.1.032
  13. Zhang H, Shen P, Cheng Y. Identification and determination of the major constituents in traditional Chinese medicine Si-Wu-Tang by HPLC coupled with DAD and ESI-MS. J Pharm Biomed Anal. 2004; 34:705-13. https://doi.org/10.1016/S0731-7085(03)00650-2