Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Enhancement of Antioxidant Activities of Bark of Berberis koreana Palibin by Lactic Acid Fermentation

유산균 발효를 통한 매자나무 수피부의 항산화 활성 증진

  • Ha, Ji-Hye (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Jeong, Myoung-Hoon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Seo, Yong-Chang (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Kim, Ji-Seon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Kim, Haeng-Hoon (National Agrobiodiversity Center, NAAS, RDA) ;
  • Ahn, Ju-Hee (Department of Biomaterials Engineering, College of Bioscience and Biotechnology) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology)
  • 하지혜 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • ;
  • 서용창 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 최운용 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 김지선 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 김행훈 (농촌진흥청 농업과학기술원 농업유전자원센터) ;
  • 안주희 (강원대학교 바이오산업공학부 생물소재공학과) ;
  • 이현용 (강원대학교 바이오산업공학부 생물소재공학과)
  • Received : 2010.06.28
  • Accepted : 2010.12.07
  • Published : 2010.12.30
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Abstract

The bark of Berberis koreana Palibin was fermented by Bifidobacterium longum B6 and Lactobacillus paracasei at $37^{\circ}C$ for 72 hour, then extracted by water solvent at $100^{\circ}C$ for 180 min. Total pholyphenol and flavonoid contents were improved by fermentation process, compared to conventional water extraction. The barks of B. koreana Palibin extracts by B. longum B6 (FE-B.L.) and by L. paracasei (FE-L.P.) showed 17% and 16% cytotoxicity on human normal cell lines(HEK293) at 1.0 mg/$m{\ell}$ of the highest concentration, respectively, which was about 3~5% lower than 20% from normal extracts (NE). DPPH radical scavenging activity of the FE-B.L. and FE-L.P. were about 73% and 75.9% higher than 56.8% of NE. The highest inhibitory potency on xathine oxidase and superoxide dismutase (SOD)-like activities were also measured as 31.9% and 61.9% by adding FE-L.P. of 1.0 mg/$m{\ell}$. On tyrosinase inhibition test, the FE-L.P. showed highest activity as 75.9% at 1.0 mg/$m{\ell}$. Generally, FE-L.P. showed higher antioxidant activities as well as higher tyrosinase inhibition activity, possibly due to high contents of total pholyphenol and flavonoid. In general, fermentation of barks of B. koreana Palibin has relatively better biological activities than normal extracts. Specifically, the extracts fermented by L. paracasei showed higher activities than that from B. longum B6.

Keywords

References

  1. Blois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 181:1199-1200. https://doi.org/10.1038/1811199a0
  2. Chen JH and Ho CT. (1997). Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. Journal of Agriculture and Food Chemistry. 45:2374-2378. https://doi.org/10.1021/jf970055t
  3. Cho YH, Imm JY, Kim HY, Hong SG, Hwang SJ, Park DJ and Oh SJ. (2009). Isolation and partial characterization of isoflavone transforming Lactobacillus plantarum YS712 for potential probiotic use. Korean Journal of Food Science and Animal Resource. 29:640-646. https://doi.org/10.5851/kosfa.2009.29.5.640
  4. Cicco N, T.Lanorte M, Paraggio M, Viggiano M and Lattanzio V. (2009). A reproducible, rapid and inexpensive Folin- Ciocalteau micro-method in determining phenolics of plant methanol extracts. Microchemical Journal. 91:107-110. https://doi.org/10.1016/j.microc.2008.08.011
  5. Chung IM, Kim KH and Ahn JK. (1998). Screening of Korean medicinal and food plants with antioxidant activity. Korean Journal of Medicinal Crop Science. 6:311-322.
  6. Doll and Peto R. (1983). The cause of cancer: quantitative estimates of avoidable risks of cancer in the United States today. Journal of the National Cancer Institute. 66:1191-1308.
  7. Dunham HJ, Wiliam C, Edens FW, Casas IA and Dobrogosz WI. (1993). Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys. Poultry Science. 72:103.
  8. Fuller R. (1989). Probiotics in man and animals. Journal of Applied Bacteriology. 66:365-368. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  9. Furrie E. (2005). Probiotics and allergy. The Proceeding of the Nutrition Society. 64:465-469. https://doi.org/10.1079/PNS2005466
  10. Gibson GR and Roberfroid MB. (1995). Dietary modulation of human colonic microbiota: Introducing the concept of probiotics. Journal of Nutrition. 12:1401-1412.
  11. Harman D. (1982). In free radicals in biology V. Academic Press. New York, USA. p. 255-275.
  12. Hearing VJ. (1987). Mannalian monophenol monooxygenase (Tyrosinase): Purification, properties, and reaction catalyzed. Methods in Enzymology. 142:154-165. https://doi.org/10.1016/S0076-6879(87)42024-7
  13. Hoolihan KL. (2001). Prophylactic and therapeutic uses of probiotics: A review. Journal of the American Dietetic Association. 101:229-238. https://doi.org/10.1016/S0002-8223(01)00060-8
  14. Hyun SH, Lee JS, Lee KB and Lee JS. (2007). Antioxidative activity of Gynostemma pentaphyllum Makino extracts. Korean Journal of Food Science and Technology. 39:447-451.
  15. Jin L, Han JG, Ha JH, Jeong HS, Kwon MC, Ahn JH, Kim JC, Choi GP, Chung EK and Lee HY. (2008). Effect of immune activity on Berberis koreana Palibin by ultra high pressure low temperature process. Korean Journal of Medicinal Crop Science. 16:439-445.
  16. Jin L, Ha JH, Jeong MH, Chung EK, Chung AR, Kim JC, Ahn JH and Lee HY. (2009). Enhancement of the actioxidant and anticancer activities of Berberis koreana bark using a low temperature and high-pressure extraction process. Korean Journal of Food Science and Technology. 41:284-291.
  17. Jung HK, Kim YJ, Park BK, Park SC, Jeong YS and Hong JH. (2007). Antioxidative and antimicrobial activities of medicinal plant extracts for screening phytobiotic material. Journal of the Korean Society of Food Science and Nutrition. 36:1235-1240. https://doi.org/10.3746/jkfn.2007.36.10.1235
  18. Kim HJ, Kim JH, Son JH, Seo JH, Park SJ, Park NS and Kim SK. (2004). Characterization of bacteriocim produced by Lactobacillus bulgaricus. Journal of Microbiology and Biotechnology. 14:503-508.
  19. Kim CS, Jang DS and Che SY. (2006). Histological characteristics of Korean red ginseng in steaming processes. Korean Journal of Medicinal Crop Science. 14:36-40.
  20. Lee SJ, Park DW, Jang HG, Kim CY, Park YS, Kim TC and Heo BG. (2006). Total phenol content, electron donating ability, and tyrosinase inhibition activity of pear cut branch extract. Korean Journal of Horticulture Science and Technology. 24:338-341.
  21. Lee HB, Kim HJ, Chong MS, Cho HE, Choi YH, Lim KS and Lee KN. (2008). Physiological activity of extracts from mixed culture of medical herbs and mycelia of Tricholoma matsutake and Cordyceps militaris by fermetation. Korean Journal of Herbology. 23:1-8.
  22. Marklund S and Marklund G. (1975). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemisty. 47:469-474.
  23. Moreni MI, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  24. Park HS, Lee HS and Uhm TB. (1995). Selection of microorganisms for probiotics and their characterization. Journal of the Korean Society of Food Science and Nutrition. 125:1401-1412.
  25. Park JG and Kim SW. (1998). Molecular study of aging by free radical. Journal of Basic science. 9:103-114.
  26. Trush MA, Mimnaugh EG and Gram TE. (1982). Activation of pharmacologic agents to radical intermediates. Implication for the role of free radicals in drug action and toxicity. Biochemical Pharmacology. 31:3335-3346. https://doi.org/10.1016/0006-2952(82)90609-8
  27. Yoo SJ, Lee KB and Kwak JH. (1986). Studies on the seasonal variation of berberine contents in Berberis koreana. Korean Journal of Pharmacognosy. 17:123-128.