Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Biological Activities in the Leaf Extract of Lythrum salicaria L.

털부처꽃 잎 추출물의 생리활성탐색

  • Kim, Hee-Yeon (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Lim, Sang-Hyun (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Park, Min-Hee (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Park, Yu-Hwa (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Ham, Hun-Ju (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Lee, Ki-Yun (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Park, Dong-Sik (Functional Food & Nutrition Division, Rural Development Administration) ;
  • Kim, Kyung-Hee (Agriproduct Processing Experiment Station Gangwon-do Agricultural Research & Experiment Services) ;
  • Kim, Song-Mun (Department of Biological Environment, Kangwon National University)
  • 김희연 (강원도농업기술원 농산물이용시험장) ;
  • 임상현 (강원도농업기술원 농산물이용시험장) ;
  • 박민희 (강원도농업기술원 농산물이용시험장) ;
  • 박유화 (강원도농업기술원 농산물이용시험장) ;
  • 함헌주 (강원도농업기술원 농산물이용시험장) ;
  • 이기연 (강원도농업기술원 농산물이용시험장) ;
  • 박동식 (농촌진흥청 국립농업과학원 한식세계화연구단 기능성식품과) ;
  • 김경희 (강원도농업기술원 농산물이용시험장) ;
  • 김성문 (강원대학교 바이오자원환경학과)
  • Received : 2010.09.02
  • Accepted : 2010.12.03
  • Published : 2010.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, the bioactivities of ethanol (EELS) and water extract (WELS) from the leaf of Lythrum salicaria L. were investigated. In the anti-cancer activity, the growths of both human prostate cancer (DU145) and human colonic carcinoma cell (HT29) were inhibited up 60% by adding 10 mg/$m{\ell}$ of EELS. Anti-inflammatory activity of EELS and WELS have been evaluated on lipopolysaccharide (LPS) induced release of nitric oxide (NO) by the macrophage RAW 264.7 cells. EELS and WELS inhibited inflammatory by 57.3 and 46.9% in 10 mg/$m{\ell}$, respectively. In the anti-oxidative activity, $IC_{50}$ of DPPH radical scavenging activity was respectively 60.71 and $92.90\;{\mu}g/m{\ell}$ by EELS and WELS. In the anti-diabetic activity, $IC_{50}$ of ${\alpha}$-amylase inhibitory activity of EELS and WELS were respectively 5,250 and $5,020\;{\mu}g/m{\ell}$. $IC_{50}$ of ${\alpha}$-glucosidase inhibitory activity was 7.96 and $68.41\;{\mu}g/m{\ell}$ by EELS and WELS. In the anti-obesity, $IC_{50}$ of lipase inhibitory activity was 880 and $9,840\;{\mu}g/m{\ell}$ by EELS and WELS. Finally, EELS and WELS exhibited anti-oxidative, anti-inflammatory, anti-diabetic activity and anti-obesity. It suggests that Lythrum salicaria L. could be potentially used as a resource of bioactive materials for health functional foods.

Keywords

References

  1. Bae JH, Yu SO, Kim YM, Chon SU, Kim BW and Heo BG. (2009). Physiological activity of methanol extracts from Ligularia fischeri and their hyperplasia inhibition activity of cancer cell. Journal of Bio-Environment Control. 18:67-73
  2. Bai G, Wang D, Cao X, Xiao H, Geng P, Liu Q and Yang W. (2004). Screening $\alpha$-glucosidase inhibitors in traditional chinese medicines. Acta Scientiarum Naturalium Universitatis Nankaiensis. 37:98-102.
  3. Becker H, Scher JM, Speakman JB and Zapp J. (2005). Bioactivity guided isolation of antimicrobial compounds from Lythrum salicaria. Fitoterapia. 7:580-584.
  4. Bitou N, Nimomiya M, Tsjita T and Okuda H. (1999). Screening of lipase inhibitors from marline algae. Lipids. 34: 441-445. https://doi.org/10.1007/s11745-999-0383-7
  5. Biois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 26:1198-1201.
  6. Choi HK. (1986). A monograph of vascular hydrohytes in Korea. Seoul National University. p. 1-58.
  7. Choi OJ. (1999). Component and use of medicinal plants. Irwolseogak. p. 486.
  8. Houghton PJ and Soumyanath A. (2006). $\alpha$-Amylase inhibitory activity of some Malaysian plants used to treat diabetes with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology. 107:449-455. https://doi.org/10.1016/j.jep.2006.04.004
  9. Kim SM and Lee SW. (1992). The bibliographical study on the famine relief food of chosun-dynasty. Journal of the East Asian of Dietary Life. 2:35-55.
  10. Kim YJ, Kim BH, Lee SY, Kim MS, Park CS, Rhee MS, Lee KH and Kim DS. (2006). Screening of medicinal plants for development of functional food ingredients with anti-obesity. Journal of the Korean Society for Applied Biological Chemistry. 49:221-226.
  11. Lamela M, Cadavid I Gato A and Calleja JM. (1985). Effects of Lythrum salicaria in normoglycemic rats. Journal of Ethnopharmacology. 14:83-91. https://doi.org/10.1016/0378-8741(85)90032-7
  12. Lamela M, Cadavid I and Calleja JM. (1986). Effects of Lythrum salicaria extracts on hyperglycemic rats and mice. Journal of Ethnopharmacology. 15:153-160. https://doi.org/10.1016/0378-8741(86)90152-2
  13. Lee BB, Park SR, Han CS, Han DY, Park E, Park HS and Lee SC. (2008). Antioxidant activity and inhibition activity against $\alpha$-amylase and $\alpha$-glucosidase of Viola mandshurica extracts. Journal of Korean Society Food Science Nutrition. 37:405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  14. Lee BB, Park SR, Han CS, Han DY, Park E, Park HS and Lee SC. (2008). Antioxidant activity and inhibition activity against $\alpha$-amylase and $\alpha$-glucosidase of Viola mandshurica extracts. Journal of Korean Society Food Science Nutrition. 37:405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  15. Lee SE, Park CG, Ahn YS, Son YD, Cha SS and Seong NS. (2009b). Antioxidative and hepatoprotective effects of Lythrum salicaria. Korean Journal of Medicinal Crop Science. 17:1-7.
  16. Lee SE, Ahn TJ, Kim GS, Kim YO, Han HS, Seo JS, Chung HY, Park CB, Cha SW, Park HK and Seong NS. (2009c). Antioxidative and anti-fibrotic properties of root extracts of Lythrum salicaria L. in CCL4-induced liver fibrosis rat model. Korean Journal of Medicinal Crop Science. 17:243-250.
  17. Lee TB. (2006). Coloured flora of Korea. Hyangmunsa, Seoul, Korea. p. 792.
  18. Lim CS, Li CY, Kim YM, Lee WY and Lee HI. (2005). The inhibitory effect of Cornus walteri extract against $\alpha$-amylase. Journal of the Korean Society for Applied Biological Chemistry. 48:103-108.
  19. Oh SJ, Hong SS, Kim YH and Koh SC. (2008). Screening of biological activities in fern plants native to Jeju island. Korean Journal of Plant Resources. 21:12-18.
  20. Park YH, Lim SH, Ham HJ, Kim HY, Jeong HN, Kim KH and Kim S. (2010). Isolation of anti-inflammatory active substance $\beta$-sitosterol from seabuckthorn(Hippophae rhamnoides L.) stem. Journal of Korean Society Food Science Nutrition. 39:980-985. https://doi.org/10.3746/jkfn.2010.39.7.980
  21. Takhtajan A. (1967). Diversity and classification of flowering plants. Columbia University Press, New York. USA. p. 284.
  22. Thnalier Z, Kosar M, Kupeli E, Calis I and Baser KHC. (2007). Antioxidant, anti-inflammatory, anti-nociceptive activities and composition of Lythrum salicaria L. extracts. Journal of Ethnopharmacology. 110:539-547. https://doi.org/10.1016/j.jep.2006.10.024
  23. Wang Z, Cai SR, He YL, Zhan WH, Chen CQ, Cui J, Wu WH, Wu H, Song W, Watanabe J, Kawabata J and Kurihara H. (1997). Isolation and identification of $\alpha$- glucosidase inhibitors from Tochu-cha. Biosci Biotechnol Biochem. 61:177-178. https://doi.org/10.1271/bbb.61.177
  24. Wang HZ, Chang CH, Lin CP and Tsai MC. (2006). Using MTT viability assay to test the cytotoxicity of antibiotics and steroid to cultured porcine corneal endothelial cells. Journal of Ocular Pharmacology and Therapeutics. 12:35-43.
  25. Xu ml, Hu JH, Wang L, Kim HS, Jin CW and Cho DH. (2010). Antioxidant and anti-diabetes activity of extracts from Machilus thunbergii S. et Z.. Korean Journal of Medicinal Crop Science. 18:34-39.
  26. Yoo MH. (2008). A taxonomic study of the Korea Lythraceae. Sungkyunkwan University Press, Gyeonggi. Korea. p. 48.
  27. Yoon SY, Lee SY, Kim KBWR, Song EJ, Kim SJ, Lee SJ, Lee CJ and Ahn DH. (2009). Anti-microbial activity of the solvent extract from different parts of Orostachys japonicus Journal of Korean Society Food Science Nutrition. 38:14-18. https://doi.org/10.3746/jkfn.2009.38.1.014