Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Enhancement of Antimicrobial Activity of Nano-Encapsulated Horseradish Aqueous Extracts Against Food-Borne Pathogens

고추냉이 수용성 추출물의 나노 입자화를 통한 식중독 미생물에 대한 항균 활성 증진

  • Seo, Yong-Chang (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Woon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Ji-Seon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Zou, Yun-Yun (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Choon-Geun (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Shin, Il-Shik (Faculty of Marine Bioscience and Technology, Kangnung-Wonju National University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
  • 서용창 (강원대학교 BT특성화학부(대학)) ;
  • 최운용 (강원대학교 BT특성화학부(대학)) ;
  • 김지선 (강원대학교 BT특성화학부(대학)) ;
  • 조윤윤 (강원대학교 BT특성화학부(대학)) ;
  • 이춘근 (강원대학교 BT특성화학부(대학)) ;
  • 안주희 (강원대학교 BT특성화학부(대학)) ;
  • 신일식 (강릉원주대학교 해양생명공학부) ;
  • 이현용 (강원대학교 BT특성화학부(대학))
  • Received : 2010.09.10
  • Accepted : 2010.11.22
  • Published : 2010.12.30
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Abstract

This work was to improve antimicrobial activities of horseradish by encapsulated with edible biopolymers such as lecithin and gelatin since it has been difficult to directly use horseradish extracts into foods and food containers due to its strong and undesirable flavors. It was shown that most of the nanoparticles containing the extracts were well formed in round shape with below 400 nm diameter as well as fairly stable and less odd flavors in various pH ranges by measuring zeta potentials. The encapsulation efficiencies of nanoparticles were estimated as 66.6% and 53.4% for lecithin and gelatin, respectively. Minimal Inhibitory Concentration (MIC) of both nanoparticles against G(+), Listeria monocytogenes and G(-), Salmonella typhimurium were also measured as 79 ppm based on AIT concentrations in the extracts, whose activities were about 65% higher than the case of adding crude extract. It was also found that the nanoparticles efficiently penetrated into the cell membrane and started to destruct the cells after 6 hours cultivation under Transmision Electron Microscopy observation. These results prove that the nano-encapsulation of the horseradish extracts can be employed to directly treat into the foods and food containers for antimicrobial purposes with the aids of aerosolization system, by using small amounts of the extracts and having less flavors due to masking effects of nanoparticles.

Keywords

References

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