Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

The Preliminary Study on the Changes of Mammary Tissue Oxygenation During Zusanli($ST_{36}$) Acupuncture Monitored by Diffuse Optical Imaging

Diffuse Optical Imaging으로 측정한 족삼리($ST_{36}$) 자침이 유방조직 산소공급 변화에 대한 기초 연구

  • Cho, Myeong-Rae (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Kim, Jae-Gwan (The Beckman Laser Institute, University of California)
  • 조명래 (동신대학교 한의과대학 침구학교실) ;
  • 김재관 (캘리포니아대학교 어바인캠퍼스 Beckman 레이저연구소)
  • Received : 2010.10.29
  • Accepted : 2010.12.03
  • Published : 2010.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

목적 : 족삼리($ST_{36}$) 자침이 유방 조직에 미치는 혈역학적 변화를 측정하기 위하여 diffuse optical imaging 기법을 사용하였다. 방법 : 실험에 사용한 쥐는 자침을 하지 않은 대조군 7마리, 자침을 한 실험군 7마리로 총 2개의 군으로 나누었다. 몸무게 170g 정도의 건강한 암컷 쥐는 100% 산소와 1.5% isoflurane을 혼합한 것을 이용하여 마취 시켰다. 자침은 경골 조면에서 2mm 외측에 위치한 지점인 족삼리($ST_{36}$)에 20분간 시행하였다. 자침을 시작할 때와 자침 후 10분에 각각 침을 5회 회전하였다. Diffuse optical imaging system을 이용하여 자침하는 동안 의 산화혈색소(OHb), 탈산소혈색소(RHb), 총 혈색소(THb)와 조직 산소 포화도($StO_2$)의 변화를 측정했다. 결과 : 실험 결과 족삼리($ST_{36}$) 자침을 시행한 실험군에서 대조군에 비하여 유의성이 있는 OHb, RHb, THb의 증가와 유의성이 없는 $StO_2$의 감소가 나타났다. 결론 : 이러한 결과 족삼리($ST_{36}$) 자침이 혈류를 증가시키고 동시에 세포 대사 활동을 증가 시키는 것을 알 수 있었다. 또한 diffuse optical imaging 기법으로 족삼리($ST_{36}$) 자침 시의 유방 조직의 산소공급과 혈류량의 변화를 확인할 수 있었고, 이는 비침습적으로 자침의 효과를 측정하는 데 활용될 수 있을 것으로 생각된다.

Keywords

References

  1. Langevin HM, Churchill DL, Cipolla MJ. Mechanical signaling through connective tissue : a mechanism for the therapeutic effect of acupuncture. The FASEB journal. 2001 ; 15(12) : 2275-82 https://doi.org/10.1096/fj.01-0015hyp
  2. Langevin HM, Yandow JA. Relationship of acupuncture points and meridians to connective tissue planes. The anatomical record. 2002 ; 269(6) : 257-65. https://doi.org/10.1002/ar.10185
  3. Uchida S, Kagitani F, Suzuki A, Aikawa Y. Effect of acupuncture-like stimulation on cortical cerebral blood flow in anesthetized rats. The Japanese journal of physiology. 2000 ; 50(5) : 495-507. https://doi.org/10.2170/jjphysiol.50.495
  4. Litscher G, Schwarz G, Sandner-Kiesling A, Hadolt I, Eger E. Effects of acupuncture on the oxygenation of cerebral tissue. Neurological Research. 1998 ; 20 Suppl 1 S28-32.
  5. Banzer W, Hübscher M, Seib M, Vogt L. Shorttime effects of laser needle stimulation on the peripheral microcirculation assessed by laser Doppler spectroscopy and near-infrared spectroscopy. Photomedicine and laser surgery. 2006 ; 24(5) : 575-80. https://doi.org/10.1089/pho.2006.24.575
  6. Zhang X, Toronov V, Webb A. Spatial and temporal hemodynamic study of human primary visual cortex using simultaneous functional MRI and diffuse optical tomography. IEEE Engineering in medicine and biology society conference proceedings. 2005 ; 1 : 727-30.
  7. Joseph DK, Huppert TJ, Franceschini MA, Boas DA. Diffuse optical tomography system to image brain activation with improved spatial resolution and validation with functional magnetic resonance imaging. Applied optics. 2006 ; 45(31) : 8142-51. https://doi.org/10.1364/AO.45.008142
  8. Yu G, Durduran T, Lech G, Zhou C, Chance B, Mohler ER, Yodh AG. Time-dependent blood flow and oxygenation in human skeletal muscles measured with noninvasive near-infrared diffuse optical spectroscopies. Journal of biomedical optics. 2005 ; 10(2) : 024027. https://doi.org/10.1117/1.1884603
  9. Zhao H, Gao F, Tanikawa Y, Homma K, Yamada Y. Time-resolved diffuse optical tomographic imaging for the provision of both anatomical and functional information about biological tissue. Applied optics. 2005 ; 44(10) : 1905-16. https://doi.org/10.1364/AO.44.001905
  10. Tromberg BJ, Shah N, Lanning R, Cerussi A, Espinoza J, Pham T, Svaasand L, Butler J. Non-invasive in vivo characterization of breast tumors using photon migration spectroscopy. Neoplasia. 2000 ; 2(1-2) : 26-40.
  11. Xu RX, Young DC, Mao JJ, Povoski SP. A prospective pilot clinical trial evaluating the utility of a dynamic near-infrared imaging device for characterizing suspicious breast lesions. Breast cancer research. 2007 ; 9(6) : R88.
  12. Boverman G, Fang Q, Carp SA, Miller EL, Brooks DH, Selb J, Moore RH, Kopans DB, Boas DA. Spatio-temporal imaging of the hemoglobin in the compressed breast with diffuse optical tomography. Physics in medicine & biology. 2007 ; 52(12) : 3619-41. https://doi.org/10.1088/0031-9155/52/12/018
  13. Cerussi A, Hsiang D, Shah N, Mehta R, Durkin A, Butler J and Tromberg BJ, Predicting res ponse to breast cancer neoadjuvant chemotherapy using diffuse optical spectroscopy, Proc Natl Acad Sci USA. 2007 ; 104(10) : 4014-9. https://doi.org/10.1073/pnas.0611058104
  14. Gao W, Huang YX, Chen H, Zhao NX, Sun DY, Zhang HX, Wang QL. Regulatory mechanism of electroacupuncture on the stomach channel-brain gut peptide-immune network. Shijie Huaren Xiaohua Zazhi. 2001 ; 9 : 279-83.
  15. Li YM, Huang YX. The effects of brain gut peptides and cytokines on the acupuncture's modulatory mechanism in the gastrointestinal immunity. Shijie Huaren Xiaohua Zazhi. 2001 ; 9 : 329-32.
  16. Xu GS, Yang YX, Liu Y, Zhu QF, Zhang FB. The mechanism of acupuncture and moxibustion effect on gastrointestine. Shijie Huaren Xiaohua Zazhi. 2000 ; 8 : 27.
  17. Zhang J, Huang YX, Gao W, Pan BR, Wang JJ, Li YM, Wang QL. Effects of acu-poin electroacupuncture on gastrointestinalmucosa immunologic function in rats. Shijie Huaren Xiaohua Zazhi. 2001 ; 9 : 1116-9.
  18. Tian L, Huang YX. The modulatory significance of electroacupuncture on cytokines in ulcerative colitis. Shijie Huaren Xiaohua Zazhi. 2001 ; 9 : 1435-8.
  19. Vickers AJ, Straus DJ, Fearon B, Cassileth BR. Acupuncture for postchemotherapy fatigue : a phase II study. Journal of clinical oncology. 2004 ; 22(9) : 1731-5. https://doi.org/10.1200/JCO.2004.04.102
  20. Hai-bo Z. Effect of Puncturing Neiguan(PC6) and Zusanli(ST36) on Chemotherapy-related Vomiting from Platinum-involved Therapeutic Project : A Report of 26 Cases. Zhen Jiu Tui Na Yi Xue(Ying Wen Ban). 2006 ; 4(4) : 233-5.
  21. Vaupel P, Thews O, Hoeckel M. Treatment resistance of solid tumors : role of hypoxia and anemia. Med Oncol. 2001 ; 18 : 243-59. https://doi.org/10.1385/MO:18:4:243
  22. Vaupel P, Höckel M. Tumor hypoxia and therapeutic resistance. In : Nowrousian MR, ed. Recombinant Human Erythropoietin(rhEPO) in Clinical Oncology : Berlin. Heidelberg, New York : Springer. 2002 : 127-46.
  23. Teicher BA. Physiologic mechanisms of therapeutic resistance. Blood flow and hypoxia. Hematol Oncol Clin North Am. 1995 ; 9 : 475-506.
  24. Henderson BW, Fingar VH. Relationship of tumor hypoxia and response to photodynamic treatment in an experimental mouse tumor. Cancer research. 1987 ; 47(12) : 3110-4.
  25. Wang HW, Putt ME, Emanuele MJ, Shin DB, Glatstein E, Yodh AG, Busch TM. Treatmentinduced changes in tumor oxygenation predict photodynamic therapy outcome. Cancer research. 2004 ; 64(20) : 7553-61. https://doi.org/10.1158/0008-5472.CAN-03-3632
  26. Powell ME, Collingridge DR, Saunders MI et al. Improvement in human tumour oxygenation with carbogen of varying carbon dioxide concentrations. Radiotherapy and Oncology. 1999 ; 50(2) : 167-71. https://doi.org/10.1016/S0167-8140(98)00123-6
  27. Horsman MR, Nordsmark M, Khalil AA, Hill SA, Chaplin DJ, Siemann DW, Overgaard J. Reducing acute and chronic hypoxia in tumours by combining nicotinamide with carbogen breathing. Acta oncologica. 1994 ; 33(4) : 371-6. https://doi.org/10.3109/02841869409098431
  28. Becker A, Kuhnt T, Liedtke H, Krivokuca A, Bloching M, Dunst J. Oxygenation measurements in head and neck cancers during hyperbaric oxygenation. Strahlentherapie und Onkologie. 2002 ; 178(2) : 105-8. https://doi.org/10.1007/s00066-002-0892-0
  29. Brizel DM, Hage WD, Dodge RK, Munley MT, Piantadosi CA, Dewhirst MW. Hyperbaric oxygen improves tumor radiation response significantly more than carbogen/nicotinamide. Radiation Research. 1997 ; 147(6) : 715-20. https://doi.org/10.2307/3579485
  30. Harrison L. Hypoxia and Anemia : Factors in Decreased Sensitivity to Radiation Therapy and Chemotherapy? The oncologist. 2004 ; 9 (suppl_5) : 31-40. https://doi.org/10.1634/theoncologist.9-90005-31
  31. Janssen HL, Haustermans KM, Balm AJ, Begg AC. Hypoxia in head and neck cancer : how much, how important?. Head & neck. 2005 ; 27(7) : 622-38. https://doi.org/10.1002/hed.20223
  32. Cuccia DJ, Bevilacqua F, Durkin AJ, Tromberg BJ. Modulated imaging : quantitative analysis and tomography of turbid media in the spatialfrequency domain. Optics letters. 2005 ; 30(11) : 1354-6. https://doi.org/10.1364/OL.30.001354
  33. National Institute of Health. Acupuncture. NIH Consensus Statement Online. 1997 Nov 3-5 ; 15(5) : 1-34.
  34. Han Z, Jiang YH, Wan Y, Wang Y, Chang JK, Han JS. Endomorphin-1 mediates 2Hz but not 100Hz electroacupuncture analgesia in the rat. Neuroscience Letters. 1999 ; 274(2) : 75-8. https://doi.org/10.1016/S0304-3940(99)00670-9
  35. Clement-Jones V, McLoughlin L, Tomlin S, Besser GM, Rees LH, Wen HL. Increased beta-endorphin but not met-enkephalin levels in human cerebrospinal fluid after acupuncture for recurrent pain. Lancet. 1980 ; 2(8201) : 946-9.
  36. Janssens LA, Rogers PA, Schoen AM. Acupuncture analgesia : a review. The Veterinary record. 1988 ; 122(15) : 355-8. https://doi.org/10.1136/vr.122.15.355
  37. Joos S, Schott C, Zou H, Daniel V, Martin E. Immunomodulatory effects of acupuncture in the treatment of allergic asthma : a randomized controlled study. The journal of alternative and complementary medicine. 2000 ; 6(6) : 519-25. https://doi.org/10.1089/acm.2000.6.519
  38. Cabýoglu MT, Ergene N, Tan U. The treatment of obesity by acupuncture. International journal of neuroscience. 2006 ; 116(2) : 165-75. https://doi.org/10.1080/00207450500341522
  39. Cabýoglu MT, Ergene N, Tan U. The Mechanism of Acupuncture and Clinical Applications. International Journal of Neuroscience. 2006 ; 116(2) : 115-25. https://doi.org/10.1080/00207450500341472
  40. Litscher G, Wang L, Huber E, Nilsson G. Changed skin blood perfusion in the fingertip following acupuncture needle introduction as evaluated by laser Doppler perfusion imaging. Lasers in medical science. 2002 ; 17(1) : 19-25. https://doi.org/10.1007/s10103-002-8262-9
  41. Sandner-Kiesling A, Litscher G, Voit-Augustin H, James RL, Schwarz G. Laser doppler flowmetry in combined needle acupuncture and moxibustion : a pilot study in healthy adults. Lasers in medical science. 2001 ; 16(3) : 184-91. https://doi.org/10.1007/PL00011353
  42. Litscher G. Near-infrared spectroscopy for objectifying cerebral effects of needle and laserneedle acupuncture. Spectroscopy. 2002 ; 16(3) : 335- 42.
  43. Litscher G. Bioengineering assessment of acupuncture, part 5 : cerebral near-infrared spectroscopy. Critical reviews in biomedical engineering. 2006 ; 34(6) : 439-57. https://doi.org/10.1615/CritRevBiomedEng.v34.i6.10