Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Effects of testosterone on the orchidectomized rats

  • Received : 2010.11.13
  • Accepted : 2010.12.28
  • Published : 2010.12.30
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Abstract

The aim of this study was to determine the changes of body weight, organ weight, hematological values and biochemical parameters by testosterone (Testos) on the orchidectomized (Orch) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. Orch group received operation and no treatment. Orch+Testos received operation and testosterone. The body weights of each group increased, but that of Orch+Testos group was significantly lower in Orch+Testos group than in all the other groups. There were significant differences (P<0.05, P<0.001) of body weights between Orch+Testos group and all the other groups. Also, organ weights such as heart, liver, spleen and kidney were measured. The heart weights were significantly lower (P<0.001) in the Orch+Testos group than in all the other groups. The liver weights in the Orch+Testos group were significantly differences in comparison with those in the Sham (P<0.001) and Orch group (P<0.05). On the other hand, there were no significantly differences in the organ weights of spleen and kidney between the Orch+Testos group and the any other groups. The hematological values of white blood cell (WBC), red blood cell (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were no significant differences in any other groups. The concentrations of serum total protein and albumin increased significantly (P<0.05) in the Orch+Testos group as compared to that in the Orch group. However, there were no significant differences in Ca, IP and Mg in any other groups. We conclude that testosterone was significantly decreased the body weight in the orchidectomized rats. Our findings suggest that testosterone may influence the process of lipid packaging and absorption in the orchidectomized rats.

Keywords

References

  1. Basaria S. 2010. Androgen abuse in athletes: detection and consequences. J Clin Endocrinol Metab 95(4): 1533-1543. https://doi.org/10.1210/jc.2009-1579
  2. Behre HM, Kliesch S, Leifke E, Link TM, Nieschlag E. 1997. Long-term effect of testosterone therapy on bone mineral density in hypogonadal men. J Clin Endocrinol Metab 82(8): 2386-2390. https://doi.org/10.1210/jc.82.8.2386
  3. Carani C, Qin K, Simoni M, Faustini-Fustini M, Serpente S, Boyd J, Korach KS, Simpson ER. 1997. Effect of testosterone and estradiol in a man with aromatase deficiency. N Engl J Med 337(2): 91-95. https://doi.org/10.1056/NEJM199707103370204
  4. Clarke BL, Khosla S. 2009. Androgens and bone. Steroids 74(3): 296-305. https://doi.org/10.1016/j.steroids.2008.10.003
  5. del Campo L, Sagredo A, Aras-Lopez R, Balfagon G, Ferrer M. 2008. Orchidectomy increases the formation of non-endothelial thromboxane A2 and modulates its role in the electrical field stimulation-induced response in rat mesenteric artery. J Endocrinol 197(2): 371-379. https://doi.org/10.1677/JOE-07-0647
  6. Erben RG, Eberle J, Stahr K, Goldberg M. 2000. Androgen deficiency induces high turnover osteopenia in aged male rats: a sequential histomorphometric study. J Bone Miner Res 15(6): 1085-1098. https://doi.org/10.1359/jbmr.2000.15.6.1085
  7. Evuarherhe O, Leggett JD, Waite EJ, Kershaw YM, Atkinson HC, Lightman SL. 2009. Organizational role for pubertal androgens on adult hypothalamic-pituitary-adrenal sensitivity to testosterone in the male rat. J Physiol 587(12): 2977-2985. https://doi.org/10.1113/jphysiol.2008.168393
  8. Ewing LL, Wing TY, Cochran RC, Kromann N, Zirkin BR. 1983. Effect of luteinizing hormone on Leydig cell structure and testosterone secretion. Endocrinology 112(5): 1763-1769. https://doi.org/10.1210/endo-112-5-1763
  9. Gentile MA, Nantermet PV, Vogel RL, Phillips R, Holder D, Hodor P, Cheng C, Dai H, Freedman LP, Ray WJ. 2010. Androgen-mediated improvement of body composition and muscle function involves a novel early transcriptional program including IGF1, mechano growth factor, and induction of {beta}-catenin. J Mol Endocrinol 44(1): 55-73. https://doi.org/10.1677/JME-09-0048
  10. Guo CY, Jones TH, Eastell R. 1997. Treatment of isolated hypogonadotropic hypogonadism effect on bone mineral density and bone turnover. J Clin Endocrinol Metab 82(2): 658-665. https://doi.org/10.1210/jc.82.2.658
  11. Hope WG, Ibarra MJ, Thomas ML. 1992. Testosterone alters duodenal calcium transport and longitudinal bone growth rate in parallel in the male rat. Proc Soc Exp Biol Med 200(4): 536-541.
  12. Keeney DS, Mendis-Handagama SM, Zirkin BR, Ewing LL. 1988. Effect of long term deprivation of luteinizing hormone on Leydig cell volume, Leydig cell number, and steroidogenic capacity of the rat testis. Endocrinology 123(6): 2906-2915. https://doi.org/10.1210/endo-123-6-2906
  13. Sarkar NN. 2009. Hormonal profiles behind the heart of a man. Cardiol J 16(4): 300-306.
  14. Wakley GK, Schutte HD Jr, Hannon KS, Turner RT. 1991. Androgen treatment prevents loss of cancellous bone in the orchidectomized rat. J Bone Miner Res 6(4): 325-330.
  15. Vandenput L, Swinnen JV, Van Herck E, Verstuyf A, Boonen S, Bouillon R, Vanderschueren D. 2002. The estrogen receptor ligand ICI 182,780 does not impair the bonesparing effects of testosterone in the young orchidectomized rat model. Calcif Tissue Int 70(3): 170-175. https://doi.org/10.1007/s00223-001-2065-z
  16. Zirkin BR, Chen H. 2000. Regulation of Leydig cell steroidogenic function during aging. Biol Reprod 63(4): 977-981. https://doi.org/10.1095/biolreprod63.4.977
  17. Zuckerman-Levin N, Frolova-Bishara T, Militianu D, Levin M, Aharon-Peretz J, Hochberg Z. 2009. Androgen replacement therapy in Turner syndrome: a pilot study. J Clin Endocrinol Metab 94(12): 4820-4827. https://doi.org/10.1210/jc.2009-0514