Journal of Soil and Groundwater Environment (한국지하수토양환경학회지:지하수토양환경)

Korean Society of Soil and Groundwater Environment (한국지하수토양환경학회)
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Denitrification by a Heterotrophic Denitrifier with an Aid of Slowly Released Molasses

고체 당밀정화제와 종속영양 탈질미생물을 이용한 질산염 제거

  • Lee, Byung-Sun (Rural Research Institute, Korea Rural Community Corporation) ;
  • Lee, Kyu-Yeon (Dept. of Civil and Environmental Engineering, Seoul National University) ;
  • Shin, Do-Yun (Dept. of Civil and Environmental Engineering, Seoul National University) ;
  • Choi, Jong-Hak (Rural Research Institute, Korea Rural Community Corporation) ;
  • Kim, Young-Jin (Dept. of Civil and Environmental Engineering, Seoul National University) ;
  • Nam, Kyoung-Phile (Dept. of Civil and Environmental Engineering, Seoul National University)
  • 이병선 (한국농어촌공사 농어촌연구원) ;
  • 이규연 (서울대학교 건설환경공학부) ;
  • 신도연 (서울대학교 건설환경공학부) ;
  • 최종학 (한국농어촌공사 농어촌연구원) ;
  • 김영진 (서울대학교 건설환경공학부) ;
  • 남경필 (서울대학교 건설환경공학부)
  • Received : 2010.04.02
  • Accepted : 2010.07.26
  • Published : 2010.08.31
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Abstract

This study was conducted to determine the potential applicability of slowly released molasses (SRM) to treat nitratecontaminated groundwater. SRM was made by dispersing molasses in hydroxy propyl methyl cellulose-silicamicrocrystalline cellulose matrix. Column test indicated that SRM could continuously release molasses with slowly decreasing release rates of $64.6mg-COD/L{\cdot}h$ up to 65 hrs, $12.1mg-COD/L{\cdot}h$ up to 215 hrs, and $4.4mg-COD/L{\cdot}h$up to 361 hrs. A batch test using an isolated indigenous heterotrophic denitrifier Pseudomonas sp. KY1 having nitrite reductase (nirK) and liquid molasses demonstrated that the bacterium decreased 100 mg-N/L of nitrate to less than 10 mg-N/L at the C/N ratio of 10/1 in 48 hours. In a Pseudomonas sp. KY1-attached Ottawa sand column which continuously received molasses from a SRM-containing reservoir, the bacterium successfully removed nitrate from 20 mg-N/L to 3 mg-N/L during the 361 hours of column operation. The results showed the possibility that SRM can be used as a reliable, longterm extra carbon source for indigenous heterotrophic denitrifiers.

Keywords

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