Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity of Methanol Extracts from the Genus Lespedeza

싸리속 식물 메탄올 추출물의 항산화 활성

  • Kim, Sang-Min (Natural Product Research Center, KIST Gangneung Institute) ;
  • Jung, Yu-Jin (Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University) ;
  • Pan, Cheol-Ho (Natural Product Research Center, KIST Gangneung Institute) ;
  • Um, Byung-Hun (Natural Product Research Center, KIST Gangneung Institute)
  • 김상민 (한국과학기술연구원, 강릉분원, 천연물소재연구센터) ;
  • 정유진 (강릉원주대학교 해양생물공학) ;
  • 판철호 (한국과학기술연구원, 강릉분원, 천연물소재연구센터) ;
  • 엄병헌 (한국과학기술연구원, 강릉분원, 천연물소재연구센터)
  • Received : 2010.02.09
  • Accepted : 2010.03.12
  • Published : 2010.05.31
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Abstract

The genus Lespedeza belonging to Leguminosae is an annual or perennial herb, which has been used as a Chinese herbal medicine. Antioxidant activity on DPPH ((1,1)-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylenebenzothiazoline-6-sulfonic acid) radicals and the contents of total polyphenol and flavonoid were analyzed with 12 methanol extracts from six Lespedeza genus plants with different plant parts. The highest content of total polyphenol was detected in the leaves of Lespedeza$\times$robusta (194.6 mg GAE/g), while the highest content of total flavonoid existed in the aerial part of Lespedeza thunbergii var. intermedia (47.9 mg QE/g). Generally, the amounts of polyphenol and flavonoid compounds were higher in the leaves than in the stems. The $SC_{50}$ value of DPPH radical scavenging activity ranged from 10.16 to 90.94 ppm, of which the strongest activity was determined in the leaves of L. robusta. Most ABTS radical scavenging activities from the investigated methanol extracts were higher than that of L-ascorbic acid, implying the excellent antioxidant activity. The radical scavenging activity in this study showed high correlation with the amount of total polyphenol rather than that of total flavonoid. These data suggest that the methanol extracts from these Lespedeza spp. could be potential candidates for natural antioxidants.

본 실험에서는 국내에 자생하는 콩과에 속하는 6종의 싸리속 식물의 각 부위별 추출물로부터 총 폴리페놀, 총 플라보노이드 함량을 측정하였고, DPPH, ABTS 라디칼 소거능을 조사하였다. 폴리페놀성 화합물은 고양싸리의 잎(LR-L)에 가장 많이 포함되어 있는 것으로 조사되었고, 플라보노이드 화합물은 풀싸리의 지상부(LTi-A)에서 가장 많이 검출되었다. 이들 시료의 항산화 활성을 조사하기 위해서 DPPH와 ABTS 라디칼에 대한 소거 활성을 조사해 본 결과 DPPH 라디칼에 대해서는 비타민 C보다 소거 활성이 적은 것으로 나타났지만, ABTS 라디칼에 대해서는 비타민 C보다 더 좋은 소거 활성을 보였다. 이들 활성과 폴리페놀, 플라보노이드 화합물간의 상관관계에서는 폴리페놀만이 양의 상관관계를 보여 폴리페놀의 농도에 따라 라디칼 소거 활성이 증가하는 것을 관찰할 수 있었다. 하지만 플라보노이드 화합물과 라디칼 소거 활성 간에는 뚜렷한 상관관계를 볼 수 없어, 싸리속 식물에서는 플라보노이드 화합물보다는 폴리페놀성화합물이 항산화에 더욱 중요한 역할을 하는 것으로 판단할 수 있었다. 이러한 결과는 최근 각광을 받고 있는 천연 항산화제의 개발에 있어 싸리속 식물이 천연 항산화제로서 좋은 소재가 될 수 있음을 보여주고 있다.

Keywords

References

  1. Yao ZY, Kan FL, Wang ET, Wei GH, Chen WX. 2002. Characterization of rhizobia that nodulate legume species of the genus Lespedeza and description of Bradyrhizobium yuanmingense sp. nov. Int J Syst Evol Micr 52: 2219-2230. https://doi.org/10.1099/ijs.0.01408-0
  2. Lee YS, Joo EY, Kim NW. 2006. Polyphenol contents and physiological activity of the Lespedeza bicolor extracts. Korean J Food Preserv 13: 616-622.
  3. Lee YS, Joo EY, Kim NW. 2005. Analysis on the components in stem of the Lespedeza bicolor. J Korean Soc Food Sci Nutr 34: 1246-1250. https://doi.org/10.3746/jkfn.2005.34.8.1246
  4. Nemoto T, Ohashi H. 1993. Seedling morphology of Lespedeza (Leguminosae). J Plant Res 106: 121-128. https://doi.org/10.1007/BF02344415
  5. Kim CK. 1993. Compositions of fatty acid, free amino acid and total amino acid of Lespedeza ${\times}$ chiisanensis T. LEE. J Korean Soc Food Nutr 22: 586-591.
  6. Lee YS, Joo EY, Kim NW. 2005. Antioxidant activity of extracts from the Lespedeza bicolor. Korean J Food Preserv 12: 75-79.
  7. Lee A, Kim BN, Zhoh CK, Shin GH. 2006. Studies on the antioxidantive and antimicrobial effects of Lespedeza bicolor extracts. J Korean Soc of Esthe Cosmec 1: 109-120.
  8. Ryu IS, Lee SJ, Lee SW, Mun YJ, Woo WH, Kim YM, Lee JC, Lim KS. 2007. Dermal bioactive properties of the ethanol extract from flowers of Lespedeza bicolor. Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology 20: 1-9.
  9. Baek SH, Kim JH, Kim DH, Lee CY, Kim JY, Chung DK, Lee CH. 2008. Inhibitory effect of dalbergioidin isolated from the trunk of Lespedeza cyrtobotrya on melanin biosynthesis. J Microbiol Biotechnol 18: 874-879.
  10. Maximov OB, Kulesh NI, Stepanenko LS, Dmitrenok PS. 2004. New prenylated isoflavanones and other constituents of Lespedeza bicolor. Fitoterapia 75: 96-98. https://doi.org/10.1016/j.fitote.2003.07.012
  11. Tan L, Zhang XF, Yan BZ, Shi HM, Du LB, Zhang YZ, Wang LF, Tang YL, Liu Y. 2007. A novel flavonoid from Lespedeza virgata (Thunb.) DC.: structural elucidation and antioxidative activity. Bioorg Med Chem Lett 17: 6311-6315. https://doi.org/10.1016/j.bmcl.2007.09.003
  12. Maya MH, Hiroyuki T, Takayuki K, Makoto K, Yu I, Toshio M. 2009. Melanin synthesis inhibitors from Lespedeza floribunda. J Nat Prod 72: 194-203. https://doi.org/10.1021/np800395j
  13. Kwon DJ, Bae YS. 2009. Flavonoids from the aerial parts of Lespedeza cuneata. Biochem Syst Ecol 37: 46-48. https://doi.org/10.1016/j.bse.2008.11.014
  14. Zhang Q, Zhang J, Shen J, Silva A, Dennis AD, Barrow CJ. 2006. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. J Appl Phycol 18: 445-450. https://doi.org/10.1007/s10811-006-9048-4
  15. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200.
  16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  17. Perron NR, Brumaghim JL. 2009. A review of the antioxidant mechanism of polyphenol compounds related to iron binding. Cell Biochem Biophys 53: 75-100. https://doi.org/10.1007/s12013-009-9043-x
  18. Lin PY, Lai HM. 2006. Bioactive compounds in legumes and their germinated products. J Agric Food Chem 54: 3807-3814. https://doi.org/10.1021/jf060002o
  19. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  20. Kuhnan J. 1976. The flavonoids. A class of semi-essential food components: their role in human nutrition. World Rev Nutr Diet 24: 117-191.
  21. Veitch NC, Grayer RJ. 2007. Flavonoids and their glycosides, including anthocyanins. Nat Prod Rep 21: 539-573. https://doi.org/10.1039/b311404j
  22. Geleijnse JM, Hollman PCH. 2008. Flavonoids and cardiovascular health: which compounds, what mechanisms? Am J Clin Nutr 88: 12-13. https://doi.org/10.1093/ajcn/88.1.12
  23. Pietta PG. 2000. Flavonoids as antioxidants. J Agric Food Chem 63: 1035-1042.
  24. Jang MJ, Ha HJ, Yoon SR, Noh JE, Kwon JH. 2006. Prediction of optimal leaching conditions for green tea. J Korean Soc Food Sci Nutr 35: 747-753. https://doi.org/10.3746/jkfn.2006.35.6.747
  25. Cai YZ, Sun M, Xing J, Luo Q, Corke H. 2006. Structureradical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sci 78: 2872-2888. https://doi.org/10.1016/j.lfs.2005.11.004
  26. Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. J Agric Food Chem 53: 1841-1856. https://doi.org/10.1021/jf030723c
  27. Yoo KH, Jeong JM. 2009. Antioxidative and antiallergic effect of persimmon leaf extracts. J Korean Soc Food Sci Nutr 38: 1691-1698. https://doi.org/10.3746/jkfn.2009.38.12.1691
  28. Kwak CS, Kim SA, Lee MS. 2005. The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. J Korean Soc Food Sci Nutr 34: 1143-1150. https://doi.org/10.3746/jkfn.2005.34.8.1143
  29. Kim DO, Lee KW, Lee HJ, Lee CY. 2002. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J Agric Food Chem 50: 3713-3717. https://doi.org/10.1021/jf020071c

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