Restorative Dentistry and Endodontics

  • 제35권1호
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  • Pages.5-12
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  • 2010
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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직접치수복조재에 따른 비글견 치수의 조직반응에 대한 연구

PULP RESPONSE OF BEAGLE DOG TO DIRECT PULP CAPPING MATERIALS: HISTOLOGICAL STUDY

  • 배지현 (분당서울대학교병원 치과보존과) ;
  • 김영균 (분당서울대학교병원 구강악안면외과) ;
  • 윤필영 (분당서울대학교병원 구강악안면외과) ;
  • 조병훈 (서울대학교 치과대학 치과보존학교실) ;
  • 최용훈 (분당서울대학교병원 치과보존과)
  • Bae, Ji-Hyun (Department of Conservative Dentistry, Seoul National University Bundang Hospital) ;
  • Kim, Young-Gyun (Department of Oral Maxillofacial Surgery, Seoul National University Bundang Hospital) ;
  • Yoon, Pil-Young (Department of Oral Maxillofacial Surgery, Seoul National University Bundang Hospital) ;
  • Cho, Byeong-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Choi, Yong-Hoon (Department of Conservative Dentistry, Seoul National University Bundang Hospital)
  • 발행 : 2010.01.29
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초록

본 연구에서는 인위적으로 노출시킨 비글견의 치수조직을 기존의 수산화칼슘제재와 MTA, 접착성 레진, 광중합형 수산화칼슘제재를 사용하여 직접치수복조한 후 각 재료에 따른 치수의 반응을 광학현미경 하에서 조직학적으로 관찰하여 비교분석하였다. 2마리 비글견의 36개 치아를 이용하여, 실험적으로 치수를 노출시키고 노출된 치수에 치수복조재를 적용한 후 와동은 복합레진으로 충전하여 직접치수복조술을 시행하였다: (1) Mineral Trioxide Aggregate (MTA: $ProRoot^{(R)}$ MTA, Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photopolymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA). 희생전 90일, 30일, 7일 전에 각 복조재별로 3개씩의 와동을 충전하였고 비글견을 희생시키고 조직시편을 제작하였다. 시편을 H&E 염색 후 광학 현미경으로 치수염증반응과 경조직 형성 정도를 관찰하였다. MTA 군은 초기에는 경조직 형성이 관찰되지 않고 치수조직의 위축과 부분괴사가 관찰되었으나 시간이 지나면서 경조직 형성이 관찰되었다. Clearfil SE Bond군은 초기에 염증세포의 침윤과 치수세포의 괴사를 관찰할 수 있었고 시간이 지날수록 치수 세포의 괴사가 더욱 진행한 양상을 보였다. Ultra-blend 군과 Dycal 군은 MTA 군과 비슷하게 초기에는 경조직 형성을 보이지 않고 중등도의 염증반응이 관찰되었으며 시간이 지나면서 경조직 형성이 관찰되었다. MTA, 수산화칼슘제재와 광중합 수산화칼슘제재는 초기의 치수염증반응 이후 조상아세포층, 상아질교 형성을 보여 직접치수복조재로 적당하였으며, 접착성 레진은 심한 염증반응과 치수 조직의 괴사 양상을 보여 직접치수복조재로 부적당하다는 결론을 얻을 수 있었다.

The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs' pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: $ProRoot^{(R)}$ MTA. Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed. In MTA calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration. In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

키워드

참고문헌

  1. Tziafas D, Smith AJ, Lesot H. Designing new treatment strategies in vital pulp therapy. J Dent 28:77-92, 2000. https://doi.org/10.1016/S0300-5712(99)00047-0
  2. Farhad A, Esfahan ZM. Calcium hydroxide: a review. Int dent J 55:293-301. 2005. https://doi.org/10.1111/j.1875-595X.2005.tb00326.x
  3. Cox CF. Re-evaluating pulp protection: calcium hydroxide liners vs cohesive hybridization. J Am Dent Assoc 125:823-831, 1994.
  4. Cox CF. Re-evaluating pulp protection: calcium hydroxide liners vs cohesive hybridization. J Am Dent Assoc 125:823-831, 1994. https://doi.org/10.14219/jada.archive.1994.0205
  5. Lee TH. The physical properties of light-activated pulp capping material. substituted for $Ca(OH)_{2}$ pastes. : Seoul National Univ.: 2007.
  6. Brannstrom M, Nyborg H. Pulpal reaction to composite resin restorations. J Prosth Dent 27:181-189, 1972. https://doi.org/10.1016/0022-3913(72)90196-5
  7. Stanley HR, Bowen RL, Cobb EN. Pulp responses to a dentin and enamel adhesive bonding procedure. Oper Dent 13:107-113, 1988.
  8. Hebling J, Giro EMA, Costa CAS. Biocompatibility of an adhesive system applied to exposed human dental pulp. J endod 25:676-682, 1999. https://doi.org/10.1016/S0099-2399(99)80354-9
  9. Costa CAS, Nascimento ABL, Teixeira HM, Fontana UF. Response of human pulps capped with a self-etching adhesive system. Dent Mater 17: 230-240, 2001. https://doi.org/10.1016/S0109-5641(00)00076-2
  10. Koliniotou-Koumpia E, Tziafas D. Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J Dent 33:639-647, 2005. https://doi.org/10.1016/j.jdent.2004.12.007
  11. Stanley HR, Swerdlow H, Buonocore MG. Pulp reactions to anterior restorative material. J Am Dent Assoc 75:132-141, 1967. https://doi.org/10.14219/jada.archive.1967.0193
  12. Torabinejad M, Pitt Ford TR. Root-end filling materials : a review. Endod Dent Traumatol 12:161-178, 1996. https://doi.org/10.1111/j.1600-9657.1996.tb00510.x
  13. Ford TR, Torabinejad M, Abedi HR, Bakland LK, Kariyawasam SP. Using mineral trioxide aggregate as a pulp-capping material. J Am Dent Assoc 127:1491-1494, 1996. https://doi.org/10.14219/jada.archive.1996.0058
  14. Faraco IM Jr, Holland R. Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dent Traumatol 17:163-166, 2001. https://doi.org/10.1034/j.1600-9657.2001.170405.x
  15. Holland R, de Souza V, Nery MJ, Otoboni Filho JA, Bernabe PF, Dezan Jr E. Reaction of rat connective tissue to implanted dentin tubules filled with mineral trioxide aggregate or calcium hydroxide. J Endod 25:161-166, 1999. https://doi.org/10.1016/S0099-2399(99)80134-4
  16. Asgary S, Mohammad JE, Masoud P, Farzin G, Hessam R. A comparative study of histologic response to different pulp capping materials and a novel endodontic cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:609-614, 2008.
  17. Torabinejad M, Hong CU, MacDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 21:349-353, 1995. https://doi.org/10.1016/S0099-2399(06)80967-2
  18. Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Patho 120:340-349, 1965.
  19. Rehman K, Saunders WP, Foye RH, Sharkey SW. Calcium ion diffusion from calcium hydroxide-containing materials in endodontically-treated teeth : an in vitro study. Int Endod J 29:271-279, 1996. https://doi.org/10.1111/j.1365-2591.1996.tb01381.x
  20. Hayashi Y. Ultrastructure of initial calcification in wound healing following pulpotomy. J Oral Pathol 11:174-180, 1982. https://doi.org/10.1111/j.1600-0714.1982.tb00153.x
  21. Yamamura T. Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound healing. J Dent Res 64:530-540, 1985. https://doi.org/10.1177/002203458506400406
  22. Goldberg F, Massone EJ, Spielberg C. Evaluation of the dentinal bridge after pulpotomy and calcium hydroxide dressing. J endod 10:318-320, 1984. https://doi.org/10.1016/S0099-2399(84)80186-7
  23. Schroder U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration. proliferation and differentiation. J Dent Res 64:541-548, 1985. https://doi.org/10.1177/002203458506400407
  24. Holland R. Pinheiro CE, de Mello W, Nery MJ, de Souza V. Histochemical analysis of the dogs' dental pulp after pulp capping with calcium, barium. and strontium hydroxides. J Endod 8:444-447, 1982. https://doi.org/10.1016/S0099-2399(82)80148-9
  25. Stanley HR, Pameijer CH. Pulp capping with a new visible-light-curing calcium hydroxide composition (Prisma VLC Dycal). Oper Dent 10:156-163, 1985.
  26. Kim JH, Hong JB, Lim BS, Cho BH. Histological evaluation of direct pulp capping with DSP-derived synthetic peptide in beagle dog. J Kor Acad Cons Dent 34:120-129, 2009. https://doi.org/10.5395/JKACD.2009.34.2.120
  27. Yun YR, Yang IS, Hwang YC, Hwang IN, Choi HR, Yoon SJ, Kim SH, Oh WM. Pulp response of mineral trioxide aggregate, calcium sulfate or calcium hydroxide. J Kor Acad Cons Dent 32: 95-101, 2007. https://doi.org/10.5395/JKACD.2007.32.2.095
  28. Goldberg M, Six N, Decup F, Buch D, Soheili Majd E, Lasfargues JJ, Salih E, Stanislawski L. Application of bioactive molecules in pulp-capping situations. Adv Dent Res 15: 91-95, 2001. https://doi.org/10.1177/08959374010150012401

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  2. Histology of dental pulp healing after tooth replantation in rats vol.35, pp.4, 2010, https://doi.org/10.5395/JKACD.2010.35.4.273
  3. Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide vol.36, pp.5, 2011, https://doi.org/10.5395/JKACD.2011.36.5.397
  4. Experimental Study of Pulp Capping Using Xenogenic Demineralized Dentin Paste vol.25, pp.3, 2016, https://doi.org/10.2485/jhtb.25.321