한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제14권4호
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  • Pages.253-259
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  • 2010
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

생쥐의 수정란 배아줄기세포와 체세포핵이식 배아줄기세포에서 각인유전자, H19, Igf2r, Snrpn의 메틸화 경향

Methylation Patterns of Imprinting Genes, H19, Igf2r, and Snrpn, in Mouse Embryonic Stem Cells and Nuclear Transferred Embryonic Stem Cells

  • 이민호 (차의과학대학교 강남차병원 유전학연구실) ;
  • 주진영 (독일 Max Planck 연구소) ;
  • 조율희 (한양대학교 의과대학 유전학연구실) ;
  • 심성한 (차의과학대학교 강남차병원 유전학연구실)
  • Lee, Min-Ho (Genetics Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University) ;
  • Ju, Jin-Young (Dept. of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine) ;
  • Cho, Youl-Hee (Dept. of Medical Genetics, College of Medicine, Hanyang University) ;
  • Shim, Sung-Han (Genetics Laboratory, Fertility Center of CHA Gangnam Medical Center, CHA University)
  • 투고 : 2010.11.14
  • 심사 : 2010.12.11
  • 발행 : 2010.12.31
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초록

DNA 메틸화 (DNA methylation)는 유전자의 발현을 조절하는 대표적인 후생학적 조절기작 (epigenetic regulation) 중에 하나이다. DNA 메틸화 양상은 생식세포 형성과정 및 배 발생단계에서 탈메틸화 (demethylation)와 de novo 메틸화의 드라마틱한 변화가 일어난다. 또한 이러한 DNA 메틸화는 배아줄기세포 (embryonic stem cells, ESCs)에서 특징적인 양상을 보이는 것으로 알려져 있다. 본 연구에서는 생쥐 수정란 유래 배아줄기세포와 체세포핵이식 배아줄기세포 (nuclear transplanted ESCs)를 이용해서 대표적 각인유전자 (imprinting genes)로 알려진 Snrpn, Igf2r, H19 유전자들에 대한 메틸화 양상을 알아보고자 하였다. 연구 결과 H19 유전자에 대해서는 DNA 메틸화 양상은 수정란 유래 배아줄기세포와 체세포핵이식 배아줄기세포에서 비슷한 경향을 보였으나, Snrpn과 Igf2r의 경우에는 체세포핵이식 배아줄기세포에서 과메틸화 (hypermethylation) 경향을 보였다.

DNA methylation is one of the major epigenetic regulations of gene expression. The DNA methylation patterns are dramatically changed during gametogenesis and embryogenesis, and especially, it has been known that embryonic stem cells show a distinct methylation pattern. In this study, we examined the methylation patterns of imprinting genes, H19, Igf2r, and Snrpn, in stem cells induced from fertilized embryo (fES) and somatic cell nuclear transferred embryo (ntES). The methylation pattern of H19 gene in both fES and ntES were similar. However, the methylation patterns of Igf2r and Snrpn in ntES (hypermethylated) were slightly different from fES cells.

키워드

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