Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지

Anti-Thrombotic Effects of Egg Yolk Lipids In Vivo

  • Cho, Hyun-Jeong (Department of Biomedical Laboratory Science, College of Medical Science, Konyang University) ;
  • Ju, Young-Cheol (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering and Regional Research Center, Inje University) ;
  • Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering and Regional Research Center, Inje University)
  • Received : 2010.11.05
  • Accepted : 2010.12.15
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the effect of egg yolk lipids (EYL) on collagen ($10\;{\mu}g/ml$)-stimulated platelet aggregation in vivo. Dietary EYL significantly inhibited collagen-induced platelet aggregation, in addition, increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), intracellular $Ca^{2+}$-antagonist as aggregation-inhibiting molecules, in collagen-stimulated platelets. These results suggest that EYL inhibits the collagen-induced platelet aggregation by up-regulating the cAMP and cGMP production. On the other hands, prothrombin time (PT) on extrinsic pathway of blood coagulation was potently prolonged by dietary EYL in vivo. These findings suggest that EYL prolongs the internal time between the conversion of fibrinogen to fibrin. Accordingly, our data demonstrate that EYL may be a crucial tool for a negative regulator during platelet activation and blood coagulation on thrombotic diseases.

Keywords

References

  1. Akoum A, Josefonvicz J, Sigot M. Anticoagulant activity of a bacterial glycopeptide. Thromb Res. 1990.60: 9-18. https://doi.org/10.1016/0049-3848(90)90335-A
  2. Anet J, Anet J, Back JF, Baker RS, Barnett D, Burley RW, Howden ME. Allergens in the white and yolk of hen's egg. A study of IgE binding by egg proteins. Int Arch Allergy Appl Immunol. 1985.77: 364-371. https://doi.org/10.1159/000233846
  3. Cho HJ, Cho JY, Rhee MH, Park HJ. Cordycepin (3'-deoxyadenosine) inhibits human platelet aggregation in a cyclic AMP- and cyclic GMP-dependent manner. Eur J Pharmacol. 2007. 558: 43-51. https://doi.org/10.1016/j.ejphar.2006.11.073
  4. Davie EW, Ratnoff OD. Waterfall sequence for intrinsic blood clotting. Science 1964.145: 1310-1312. https://doi.org/10.1126/science.145.3638.1310
  5. el-Daher SS, Eigenthaler M, Walter U, Furuichi T, Miyawaki A, Mikoshiba K, Kakkar VV, Authi KS. Distribution and activation of cAMP- and cGMP-dependent protein kinases in highly purified human platelet plasma and intracellular membranes. Thromb Haemost 1996.76: 1063-1071.
  6. Folch J, Lees M, Sloane-Starley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957.226: 497-509.
  7. Furie B, Furie BC. The molecular basis of blood coagulation. Cell 1988.53: 505-518.
  8. Garcia-Manzano A, Gonzalez-Llaven J, Lemini C, Rubio-Poo C. Standardization of rat blood clotting tests with reagents used for humans. Proc West Pharmacol Soc. 2001. 44: 153-155.
  9. Howell WH, McNamara DJ, Tosca MA, Smith BT, Gaines JA. Plasma lipid and lipoprotein responses to dietary fat and cholesterol: a meta-analysis. Am J Clin Nutr. 1997.65: 1747-1764.
  10. Jang EK, Azzam JE, Dickinson NT, Davidson MM, Haslam RJ. Roles for both cyclic GMP and cyclic AMP in the inhibition of collagen-induced platelet aggregation by nitroprusside. Br J Haematol. 2002. 117: 664-675. https://doi.org/10.1046/j.1365-2141.2002.03479.x
  11. Langeland T. A clinical and immunological study of allergy to hen's egg white. IV. Specific IGE-antibodies to individual allergens in hen's egg white related to clinical and immunological parameters in egg-allergic patients. Allergy 1983. 38: 493-500. https://doi.org/10.1111/j.1398-9995.1983.tb02358.x
  12. Rodomski MW, Palmer RMJ, Monacade S. An L-arginine/nitric oxide pathway present in human platelets regulates aggregation. Proc Natl Acad Sci. 1990. 87: 5193-5197. https://doi.org/10.1073/pnas.87.13.5193
  13. Sano Y, Sato K, Uchida M, Murata M. Blood coagulation Fibrinolysis of Rats Fed Fish Oil: Reduced Coagulation Factors Especially Involved in Intrinsic Pathway and Increased Activity of Plasminogen Activator Inhibitor. Biosic Biotechnol Biochem. 2003. 67: 2100-2105. https://doi.org/10.1271/bbb.67.2100
  14. Schwartz SM, Heinmark RL, Majesky MW. Developmental mechanisms underlying pathology of arteries. Physiol Rev. 1990. 70: 1177-1209.