Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Construction of 1H-15N Double Resonance Solid-State NMR Probe for Membrane Proteins in Aligned Bicelles

  • Park, Tae-Joon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Kim, Ji-Sun (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Um, Seung-Hoon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Kim, Yong-Ae (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
  • Received : 2010.02.06
  • Accepted : 2010.02.26
  • Published : 2010.05.20
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Abstract

$^1H-^{15}N$ heteronuclear dipolar coupling solid-state NMR experiments on lipid bilayer or bicelle samples are very useful for the structural studies of membrane proteins. However, to study these biological samples using solid-state NMR, a specific probe with high efficiency and high capability is required. In this paper, we describe the optimized design, construction, and efficiency of a 400 MHz wide-bore $^1H-^{15}N$ solid-state NMR probe with 5-mm solenoidal rf coil for high power, multi-pulse sequence experiments, such as 2D PISEMA or 2D SAMMY.

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