Bulletin of the Korean Chemical Society

  • 제31권5호
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  • Pages.1187-1191
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  • 2010
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Construction of 1H-15N Double Resonance Solid-State NMR Probe for Membrane Proteins in Aligned Bicelles

  • Park, Tae-Joon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Kim, Ji-Sun (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Um, Seung-Hoon (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies) ;
  • Kim, Yong-Ae (Deparment of Chemistry and Protein Research Center for Bio-Industry, Hankuk University of Foreign Studies)
  • 투고 : 2010.02.06
  • 심사 : 2010.02.26
  • 발행 : 2010.05.20
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초록

$^1H-^{15}N$ heteronuclear dipolar coupling solid-state NMR experiments on lipid bilayer or bicelle samples are very useful for the structural studies of membrane proteins. However, to study these biological samples using solid-state NMR, a specific probe with high efficiency and high capability is required. In this paper, we describe the optimized design, construction, and efficiency of a 400 MHz wide-bore $^1H-^{15}N$ solid-state NMR probe with 5-mm solenoidal rf coil for high power, multi-pulse sequence experiments, such as 2D PISEMA or 2D SAMMY.

키워드

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피인용 문헌

  1. H Solid-state NMR Study of Membrane Proteins for an 800 MHz NB Magnet vol.34, pp.3, 2013, https://doi.org/10.5012/bkcs.2013.34.3.823
  2. 1H-31P home-built solid-state NMR probe with a scroll coil for 400-MHz NB magnet for biological lossy sample vol.11, pp.1, 2020, https://doi.org/10.1186/s40543-020-00224-8