Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Development of SNP Markers for Domestic Pork Traceability

국내산 돼지고기의 원산지 검증을 위한 SNP Marker Set 개발

  • Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Li, Xiaoping (Department of Animal Science, Chungbuk National University) ;
  • Lee, Yun-Mi (School of Biotechnology, Yeungnam University) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Kim, Tae-Hun (National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Bong-Hwan (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
  • 김상욱 (충북대학교 농업생명환경대학 축산학과) ;
  • 이소평 (충북대학교 농업생명환경대학 축산학과) ;
  • 이윤미 (영남대학교 생명공학부) ;
  • 김종주 (영남대학교 생명공학부) ;
  • 김태헌 (농촌진흥청 국립축산과학원) ;
  • 최봉환 (농촌진흥청 국립축산과학원) ;
  • 김관석 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2009.11.09
  • Accepted : 2010.02.11
  • Published : 2010.04.30
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Abstract

The purpose of the study was to develop an optimum SNP marker set to be utilized for domestic pork traceability. The study tested 51 SNP markers analyzed for origin of farm to be determined from genotypes of offspring and parents in pigs. With the simulation data through random mating population (PI), half sib mating population ($PI_{half-sib}$) and full sib mating population ($PI_{sibs}$), probability of identical genotypes were analyzed as $5.63{\times}10^{-33}$, $4.35{\times}10^{-15}$ and $1.32{\times}10^{-15}$, respectively. The 51 SNP markers also had 100% accuracy for parental determination. These results suggest that if the pig breeding stock is genotyped with the 51 SNP markers, the genotype information of individual offspring can be checked for farm origins by tracing parental sow and sire. Therefore, these SNP markers will be useful to trace the pork from production to consumption in pigs.

본 연구는 돼지고기 원산지 식별에 활용될 수 있는 최적의 SNP marker set을 개발 및 확립하기 위해 수행되었다. 선발된 51개의 SNP marker들의 효율성, 다형성 및 독립성 검증을 실시하였으며 51개의 SNP marker set은 MassARRAY method에 의해서 Multiplex-PCR panel 4개로 디자인 되었으며, 농장별, 생산조합별, 모돈별, 웅돈별로 효과적으로 고유 유전자형 지문분석이 가능하게 제작되었고, 다른형태의 SNP 유전자형 분석 플랫폼에 적용될 수 있는 적절한 마커 갯수이다. 또한 51개의 SNP marker set을 적용하여 모의 실험 및 친자감별확율을 계산하였을 때 무작위 교배 집단(PI), 반형매 교배집단($PI_{half-sib}$)과 전형매 교배집단($PI_{sibs}$)을 통해 모의 실험을 한 결과 $5.63{\times}10^{-33}$, $4.35{\times}10^{-15}$ 그리고 $1.32{\times}10^{-15}$로 분석되었으며 친자확인률에서도 모두 100%에 가까운 확률값을 나타내었다. 따라서 본 연구에서 개발된 SNP marker set을 이용하여 돈육제품의 원산지를 추적에 이용한다면 개별돼지의 고유한 DNA 지문 정보를 생성할 뿐만 아니라, 이를 통하여 모돈과 웅돈을 식별하여 농장원산지를 확인이 가능 할 수 있을 것으로 사료된다. 따라서 국내산 돼지의 생산에서부터 돈육제품으로의 소비까지 이력추적이 가능한 도구로 제공 될 것이다.

Keywords

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