Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bacteriocinogenic Potential of Newly Isolated Strains of Enterococcus faecium and Enterococcus faecalis from Dairy Products of Pakistan

  • Javed, Imran (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Ahmed, Safia (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Ali, Muhammad Ishtiaq (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Ahmad, Bashir (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Ghumro, Pir Bux (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Hameed, Abdul (Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University) ;
  • Chaudry, Ghulam Jilani (South Texas Center for Emerging Infectious Diseases, Department of Biology, University of Texas at San Antonio)
  • Published : 2010.01.31
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Abstract

The present study was carried out for the isolation of bacteriocin-producing enterococci from indigenous sources. Gram-positive enterococci are known for having the ability to produce enterocins with good antimicrobial potential. A total of 34 strains were isolated from processed dairy products of Pakistan and seven out of them were found to be member of genus Enterococcus on selective enumeration. Biochemical and molecular characterization revealed that four of these isolates (IJ-03, IJ-07, IJ-11, and IJ-12) were Enterococcus faecalis and three (IJ-06, IJ-21, and IJ-31) were Enterococcus faecium. Local processed cheese was the source of all enterococcal isolates, except E. faecium IJ-21 and IJ-31, which were isolated from indigenous yoghurt and butter samples, respectively. Bacterial isolates were sensitive to commonly used antibiotics except methicillin and kanamycin. They also lacked critical virulence determinants, mainly cytolysin (cyl), gelatinase (gel), enterococcal surface protein (esp), and vancomycin resistance (vanA and vanB). Polymerase chain reaction amplification identified that enterocin A and P genes were present in the genome of E. faecium IJ-06 and IJ-21, whereas the E. faecium IJ-31 genome showed only enterocin P genes. No amplification was observed for genes that corresponded with the enterocins 31, AS-48, L50A, and L50B, and ent 1071A and 1071B. There were no signals of amplification found for E. faecalis IJ-11, indicating that the antimicrobial activity was because of an enterocin different from those checked by PCR. Hence, the indigenous bacterial isolates have great potential for bacteriocin production and they had antibacterial activity against a variety of closely related species.

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References

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