Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography

RP titanium cap과 rhBMP-2를 이용하여 형성된 신생골의 미세전산화단층촬영을 이용한 평가

  • Han, Man-Seung (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Jung, Seung-Gon (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Bang-Sin (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Yang, Ji-Woong (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Kook, Min-Suk (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Park, Hong-Ju (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Ryu, Sun-Youl (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University) ;
  • Oh, Hee-Kyun (Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University)
  • 한만승 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 정승곤 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 김방신 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 양지웅 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 국민석 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 박홍주 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 유선열 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소) ;
  • 오희균 (전남대학교 치의학전문대학원 구강악안면외과학교실, 2단계 Brain Korea 21 (BK21), 전남대학교 치의학연구소)
  • Received : 2010.07.09
  • Accepted : 2010.12.14
  • Published : 2010.12.31
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Abstract

Introduction: This study examined the effect of recombinant human bone morphogenetic protein (rhBMP)-2 and $\beta$-tricalcium phosphate ($\beta$-TCP) on new bone formation in a rabbit calvarium using a rapid prototype titanium cap (RP Ti cap). Materials and Methods: Eight New Zealand white rabbits were used in this study. Hemispherical RP Ti caps (10 mm in diameter) were implanted subperiosteally on the rabbit calvaria. $\beta$-TCP was filled in the RP Ti cap in the control group, and rhBMP-2 soaked $\beta$-TCP was used in experimental group. The rabbits were sacrificed 2 and 4 weeks after the operation. The volume and pattern of newly formed bone was analyzed by micro computed tomography (CT). Results: Macroscopically, there were no abnormal findings in any of the animals. The micro CT images revealed new bone from the calvaria that expanded gradually toward the top of the titanium cap, particularly along the inner surface of the titanium cap in the experimental group at 4 weeks after grafting. There was no significant difference in new bone volume ratio between the control and experimental groups at 2 weeks after grafting. There was a statistically significant difference in the new bone volume ratio between the experimental ($14.1{\pm}1.8\;%$) and control ($7.2{\pm}1.5\;%$) groups at 4 weeks after grafting (P<0.01). Conclusion: The RP Ti cap can effectively guide new bone formation and rhBMP-2 can induce the new bone formation.

Keywords

References

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