Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Neuronal Cell Protective Effects of Methanol Extract from Cheonggukjang Using in vitro System

In vitro system에서 청국장 메탄올 추출물의 신경세포 보호효과

  • Jeong, Chang-Ho (Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Kwak, Ji-Hyun (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Ji-Hye (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Gwi-Nam (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Hee-Rok (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho-Jin (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 정창호 (경희대학교 생명과학대학 식품공학과) ;
  • 곽지현 (경상대학교 농업생명과학대학 식품공학과.농업생명과학연구원) ;
  • 김지혜 (경상대학교 농업생명과학대학 식품공학과.농업생명과학연구원) ;
  • 최귀남 (경상대학교 농업생명과학대학 식품공학과.농업생명과학연구원) ;
  • 정희록 (경상대학교 농업생명과학대학 식품공학과.농업생명과학연구원) ;
  • 허호진 (경상대학교 농업생명과학대학 식품공학과.농업생명과학연구원)
  • Received : 2010.06.08
  • Accepted : 2010.09.08
  • Published : 2010.12.31
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Abstract

In this study, the neuronal cell protective effects of methanol extract from cheonggukjang were evaluated. The proximate composition and total phenolics of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fiber, and 28.43 mg/g of total phenolics. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when methanol extract was present in the media compared to PC12 cells treated with $H_2O_2$ only. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into the medium was also inhibited. Furthermore, the inhibitory effect of the methanol extract against acetylcholinesterase was dose-dependent.

청국장 메탄올 추출물의 $H_2O_2$로 유도된 신경세포 독성에 대한 보호 효과를 조사하였다. 청국장 분말의 일반성분을 분석한 결과 조단백질 40.95%, 조지방 22.49%, 가용성 무질소물 15.99%, 수분 7.91%, 조회분 6.74% 및 조섬유 5.92% 순으로 나타났으며, 총 페놀성 화합물 함량은 28.43 mg/g이었다. $H_2O_2$로 유발된 산화적 손상에 의한 ROS 축적량을 조사한 결과 $H_2O_2$ 단독 처리구보다 청국장 메탄올 추출물 처리구에서 낮은 ROS 축적량을 보였다. $H_2O_2$로 유도된 PC12 신경세포에 대한 보호효과를 MTT 방법을 이용하여 측정한 결과 모든 시료에서 40%정도의 신경세포 보호효과를 보였고, LDH release 실험 결과 5 mg/mL 농도에서 15%정도의 LDH 방출량 저해 효과를 나타내었다. 청국장 메탄올추출물의 아세틸콜린에스터레이스 저해활성은 농도 의존적인 경향이었다. 본 연구 결과를 종합해 볼 때, 청국장 메탄올 추출물은 산화적 스트레스로부터 신경세포 보호효과 및 아세틸콜린에 스터레이스 저해활성을 나타내어 알츠하이머성 신경질환의 예방을 위한 식품으로서의 활용 가능성이 높다고 판단된다.

Keywords

References

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