Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

Proliferation and Functional Activity of Human Adipose Tissue-Derived CD146 Positive Endothelial Cells According to Culture Mediums

배지 성분에 따른 인간 지방조직기원 CD146 양성 혈관내피세포의 증식 및 기능의 평가

  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Jin-Hyun (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Cho, Hee-Young (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Jung, Myeong-Hee (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Deok-Ryong (Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Kim, Shin-Won (Department of Oral and Maxillofacial Surgery, School of Dentistry, Busan National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Busan National University) ;
  • Kim, Jong-Ryoul (Maxillofacial Center, Onhospital) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21))
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단(BK21)) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 김진현 (경상대학교병원 임상의학연구소) ;
  • 조희영 (경상대학교병원 임상의학연구소) ;
  • 정명희 (경상대학교병원 임상의학연구소) ;
  • 김덕룡 (경상대학교 의학전문대학원 생화학교실, 경상대학교 건강과학연구원 의생명과학사업단(BK21)) ;
  • 김신원 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김종렬 (온 종합병원 턱얼굴센터) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단(BK21))
  • Received : 2010.08.19
  • Accepted : 2010.10.04
  • Published : 2010.11.30
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Abstract

Purpose: This study was to examine the proliferation and function of the adipose tissue-derived endothelial cells according to different culture medium conditions. Materials and Methods: Adipose tissue-derived CD146 positive endothelial cells were cultured in according to different culture mediums (DMEM culture medium with or without osteogenic inductive agents and EBM-2 culture medium with or without osteogenic inductive agents). The proliferation and function of the adipose tissue-derived endothelial cells was examined in different culture medium conditions. Results: Adipose tissue-derived endothelial cells formed tube-like structures on Matrigel in EBM-2 culture medium with or without osteogenic inductive agents. However, the cells did not form tube-like structures on Matrigel in DMEM medium with or without osteogenic inductive agents. After 24 hours of culture, among the culture medium using EBM-2, the proliferation of the cells were promoted in EBM-2 medium without osteogenic inductive agents than in EBM-2 medium with osteogenic inductive agents. However, 72 hours of culture, the proliferation of the cells were promoted in EBM-2 medium with osteogenic inductive agents than in EBM-2 medium without osteogenic inductive agents. Conclusion: These results suggest that the proliferation and function of the adipose tissue-derived CD146 positive endothelial cells could be maintained in EBM-2 with osteogenic inductive agents.

Keywords

References

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