오매 에탄올 추출물의 항산화 활성 및 항암 활성 연구

Study on Antioxidant and Cytotoxic Activities in Ethanol Extract from Prunus mume

  • 전연희 (경북대학교 식품영양학과) ;
  • 권지은 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과, 장수생활과학연구소)
  • Jeon, Yeon-Hee (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kwon, Ji-Eun (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Mee-Ra (Dept. of Food Science and Nutrition, Center for Beautiful Aging, Kyungpook National University)
  • 투고 : 2010.09.09
  • 심사 : 2010.10.21
  • 발행 : 2010.10.31

초록

Prunus mume is well known contain many functional materials that play beneficial roles in the human body. Studies have found that many organic acids and polyphenol compounds exist in Prunus mume. In this study, content of total polyphenols and flavonoids, antioxidative activity and cytotoxicity of ethanol extract from Prunus mume were measured Contents of total polyphenolic and total flavonoid compounds in ethanol extract from Prunus mume were 16.92 mg/g and 59.95 mg/g, respectively. The $IC_{50}$ of ethanol extract from Prunus mume were $237.72 {\mu}g$/assay and $239.58 {\mu}g$/assay by DPPH and ABTS radical cation scavenging test, respectively. Additionally, ferric ion reducing antioxidant power (FRAP) value of ethanol extract from Prunus mume was 0.94 mM ($FeSO_4$ eq.) by $800 {\mu}g$/assay. Cytotoxicity of Prunus mume against five kinds of cancer cell lines increased as the extract concentration increased Especially, cytotoxicity of the ethanol extract against A-549 (lung cancer line) was higher than that against any other cancer cell line by both MTT and SRB assay. These results show that ethanol extract of Prunus mume has considerably high antioxidative and cytotoxic activities.

키워드

참고문헌

  1. 마쓰모토코사이(1999) 매실민간요법. 국일미디어, 서울. p 64.
  2. 이상희 (2002) 매화. 넥서스북, 서울. p 518-520.
  3. An BJ, Lee SA, Son JH, Kwak JH, Park JM, Lee JY (2004) Cytotoxic and antibacterial activities of Sanguisorbae officinalis L. J Korean Soc Appl Biol Chem 47: 141-145.
  4. Bae JH, Kim KJ, Kim SM, Lee WJ, Lee SJ (2000) Development of the functional beverage containing the Prunus mume extracts. Korean J Food Sci Technol 32: 713-719.
  5. Benzie F, Strain J (1996) The ferric reducing ability of plasma(FRAP) as a measure of "antioxidant power" : The FRAP assay. Anal Biochem 239: 70-76. https://doi.org/10.1006/abio.1996.0292
  6. Blios MS (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  7. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942.
  8. Cho WG, Han SK, Sin JH, Lee JW (2008) Antioxidant of heating pork and antioxidative activities of Rubus coreanus Miq. extracts. J Korean Soc Food Sci Nutr 37: 820-825. https://doi.org/10.3746/jkfn.2008.37.7.820
  9. Choi JI, Kim YJ, Kim JH, Song BS, Yoon YH, Byun MW, Kwon JH, Chun SS, Lee JW (2009) Antioxidant activities of the extract fractions from Suaeda japonica. J Korean Soc Food Sci Nutr 38: 131-135. https://doi.org/10.3746/jkfn.2009.38.2.131
  10. Choi SY, Chung MJ, Shin JH, Kim HJ, Sung NJ (2002) Effect of green tea (Camellia sinensis) and Maesil (Prunus mume) extracts on endogenous formation of N-nitrosodimethylamine. Korean J Food & Nutr 15: 16-22.
  11. Choi SY, Lim SH, KIM JS, Ha TY, Kim SR, Kang KS, Hwang IK (2005) Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Korean J Food Sci Technol 37: 549-556.
  12. Doll R, Peto R (1981) The causes of cancer : Quantitive estimates of avoiable risks of cancer in the United States today. J Natl Cancer Inst 66: 1191-1308.
  13. Folin O, Denis W (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 7: 239-243.
  14. Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals transition metals and disease. Biochem J 219: 1-14. https://doi.org/10.1042/bj2190001
  15. Han JT, Lee SY, Kim KN, Baek NI (2001) Rutin, antioxidant compound isolated from the fruit of Prunus mume. J Korean Soc Agric Chem Biotechnol 44: 35-37.
  16. Jayakasha GK, Patil BS (2007) In vitro evaluation of the antioxidant activities in fruit extracts from citron and blood orange. Food Chem 101: 410-418. https://doi.org/10.1016/j.foodchem.2005.12.038
  17. Jeong HS, Han JG, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH, Choi GP, Park UY, Lee HY (2009) Enhancement of anticancer? Activities of Ephedra sinica, Angelica gigas by ultra high pressure extraction. Korean J Medical Crop Sci 17: 102-108.
  18. Jung SE (2000) Inhibitory effect of Prunus mume extracts on the growth of liver cancer and cervical cancer cell lines. MS Thesis Keimyung University. Daegu. p 12-13.
  19. Kim EY, Bail IH, Kim JH, Kim SR, Rhyu MR (2004) Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  20. Kim HJ, Jo CH, Kim TH, Kim DS, Park MY, Byun MW (2006) Biological evaluation of the methanolic extract of Eriobotrya japonica and its irradiation effect. Korean J Food Sci Technol 38: 684-690.
  21. Kim JG, Shin M (2007) The study of Maesil (fruit of Prunus mume) and Maehwa (flower of Prunus mume) in Korean. Food Culture J Honam Area Studies 40: 79-116.
  22. Kim SI, Sim KH, Ju SY, Han YS (2009) A study of antioxidative and hypoglycemic activities of Omija (Schizandra chinensis Baillon) extract under variable extract conditions. Korean J Food & Nutr 22: 41-47.
  23. Kim YG (2004) Antioxidants. Ryo Moon Gak, Korea.
  24. Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. (2000) Comparison of the free radical-scavenging activity of propolis several regions of Argentina. J Ethnopharmacology 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  25. Park MK, Kim CH (2009) Extraction of polyphenols from apple peel using cellulase and pectinase and estimation of antioxidant activity. J Korean Soc Food Sci Nutr 38: 535-540. https://doi.org/10.3746/jkfn.2009.38.5.535
  26. Park YS (2002) Antioxidative activities and contents of polyphenolic compound of medical herb extracts. J East Asian Soc Dietary Life 12: 23-31.
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  28. Park KW, Choi SR, Shon MY, Jeoung IY, Kang KS, Lee ST, Shim KH, Seo KI (2007) Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J Korean Soc Food Sci Nutr 36: 1385-1390. https://doi.org/10.3746/jkfn.2007.36.11.1385
  29. Yim D, Singh RP, Agarwal C, Lee S, Chi H, Agarwal R (2005) A novel anticancer agents, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res 65: 1035-1044.
  30. Yin Y, Hel SI, Jung MJ, Wang MH (2009) Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Kor J Phamacogn 40: 1-5.
  31. Yook CS, Rho YS, Se SH, Leem JY, Han SH (1996) Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40: 251-261.