Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Physicochemical Properties of Dioscorea opposita Thunb. Solution Fermented by Lactobacillus bulgaricus

  • Received : 2010.07.02
  • Accepted : 2010.10.11
  • Published : 2010.10.31
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Abstract

The present study was designed to determine the physicochemical properties of yam (Dioscorea) solution fermented by L. bulgaricus, the protective effect of fermented Dioscorea opposita Thunb. on stomach lesions, and the possibility of its application to fermented dairy products. When the concentration of yam increased, the pH increased in raw powder samples but not in extracted powder samples. In 6% raw powder containing the sample, the viable cell counts showed a dramatic increase, whereas those in extracted yam powder significantly decreased in all concentrations during 32 h of fermentation. The viscosity of extracted yam powder showed a lower value than that of raw powder, which was maintained throughout the fermentation. The solution of Dioscorea opposita Thunb. raw powder showed a significantly higher allantoin content compared with the extracted powder (p<0.05). At 2% of added Dioscorea opposita Thunb., 0.65 mg/mL was contained in both samples; however, when 6% of Dioscorea opposita Thunb. was added, the amount of diosgenin increased up to 1.9 mg/mL. When 200 mg of powder/kg body wt. was injected, gastric lesions were apparently reduced compared with those of control rats. With 100 mg/kg body wt. injected into rats, the protective effect was a little lower than that in 200 mg/kg body wt. Based on these data, the present study indicated that yam powder fermented by L. bulgaricus showed a protective effect on gastric lesions in rats; therefore, it may be included as an added ingredient in yogurt manufacture.

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References

  1. Carr, F. J., Chill, D., and Maida, N. (2002) The lactic acid bacteria: A literature survey. Crit. Rev. Microobiol. 28, 281-370. https://doi.org/10.1080/1040-840291046759
  2. Chang, K. J., Park, B. J., Paek, J. H., Kim, S. L., and Park, C. H. (2005) Tuber enlargement and chemical components of yams (Dioscorea opposita Thunb.). Korean J. Plant Res. 18, 161-168.
  3. Fu, Y. C., Ferng, L. H. A., and Huang P. Y. (2006) Quantitative analysis of allantoin and allantoic acid in yam tuber, mucilage skin and bulbil of the Dioscorea species. Food Chem. 94, 541-549. https://doi.org/10.1016/j.foodchem.2004.12.006
  4. Fsu, F. L., Lin, Y. H., Lee, M. H., Lin, C. L., and Hou, W. C. (2002) Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong No. 1) and its peptic hydrolysates exhibited angiotensin converting enzyme inhibitory activities. J. Agric. Food Chem. 50, 6109-6113. https://doi.org/10.1021/jf0203287
  5. Garnick, J. J., Singh, B., and Winkley, G. (1998) Effectiveness of a medicament containing silicon dioxide, aloe, and allantoin on aphthous stomatitis. Oral Survey Oral Med. Oral Pathol. Endodon. 86, 550-556. https://doi.org/10.1016/S1079-2104(98)90344-4
  6. Gilliland, S. E. (1990) Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol. Rev. 7, 175-188. https://doi.org/10.1016/0378-1097(90)90705-U
  7. Gorbach, S. L. (1990) Lactic acid bacteria and human health. Ann. Med. 22, 37-41. https://doi.org/10.3109/07853899009147239
  8. Hou, W. C., Chen, H. J., and Lin, Y. H. (1999) Dioscorins, the major tuber storage proteins of yam (Dioscorea batatas Decne), with dehydroascorbate reductase and monodehydroascorbate reductase activities. Plant Sci. 149, 151-156. https://doi.org/10.1016/S0168-9452(99)00152-1
  9. Hou, W. C., Lee, M. H., Chen, H. J., Liang, W. L., Han, C. H., Liu, Y. W., and Lin, Y. H. (2001) Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J. Agric. Food Chem. 49, 4956-4960. https://doi.org/10.1021/jf010606m
  10. Hsu, C. C., Huang, Y. C., Yin, M. C., and Lin, S. J. (2006) Effct of yam (Dioscorea alata compared to Dioscorea japonica) on gastrointestinal function and antioxidant activity in mice. J. Food Sci. 71, 513-516.
  11. Hu, K. and Yao, X. (2003a) The cytotoxicity of methyl protodioscin against human cancer cell lines in vitro. Cancer Invest. 21, 389-393. https://doi.org/10.1081/CNV-120018230
  12. Hu, K. and Yao, X. (2003b) The cytotoxicity of methyl protoneogracillin (NSC-698793) and gracillin (NSC-698787), two steroidal saponins from the rhizomes of Dioscorea collettii var. hypoglauca, against human cancer cells in vitro. Phytother. Res. 17, 620-626. https://doi.org/10.1002/ptr.1211
  13. Jeon, J. R., Lee, J. S., Lee, C. H., Kim, J. Y., Kim, S. D., and Nam, D. H. (2006) Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model. Arch. Pharm. Res. 29, 348-353. https://doi.org/10.1007/BF02968583
  14. Kim, M. J., Kim, H. N., Kang, K. S., Baek, N. I., Kim, D. K., Kim, Y. S., Jeon, B. H., and Kim, S. H. (2004) Methanol extract of Dioscorea batatas inhibits proinflammatory cytokines and mediators in the synviocytes of rheumatoid arthritis. Int. Immunopharmacol. 4, 1489-1497. https://doi.org/10.1016/j.intimp.2004.07.001
  15. Lee, C. H. (1997) Lactic acid fermented foods and their benefits in Asia. Food Control 8, 259-269. https://doi.org/10.1016/S0956-7135(97)00015-7
  16. Liu, M. J., Wang, Z., Ju, Y., Wong, R. N., and Wu, Q. Y. (2005) Diosgenin induces cell cycle arrest and apoptosis in human leukemia K562 cells with the disruption of $Ca^{2+}$ homeostasis. Cancer Chemother. Pharmacol. 55, 79-90. https://doi.org/10.1007/s00280-004-0849-3
  17. McAnuff, M. A., Hardingm, W. W., Omoruyi, F. O., Jacobs, H., Morrison, E. Y., and Asemota, H. N. (2005) Hypoglycemic effects of steroidal sapogenins isolated from Jamaican bitter yam, Dioscorea polygonoids. Food Chem. Toxicol. 43, 1667-1672. https://doi.org/10.1016/j.fct.2005.05.008
  18. Merck index (2001) An encyclopedia of chemicals, drugs, and biologicals. 13th ed, Smith, A., Heckelman, P., and Oneil, M. J. (eds.). Merck & Co., Inc., NJ.
  19. Osuji, G. O., and Ory, R. L. (1987) Regulation of allantoin and allantoic acid degradation in the yam and sweet potato. J. Agric. Food Chem. 35, 219-223. https://doi.org/10.1021/jf00074a013
  20. Pharmacopoeia of the People's Republic of China (1997) Chemical Industry Press, Peijing, Vol. 1.
  21. Shin, K. O. Jeon, J. R., Lee, J. S., Kim, J. Y., Lee, C. H., Kim, S. CD., Yu, Y, and Nam, D. H. (2006) Lactic acid fermentation of Chinese Yam (Dioscorea batatas Decne) flour and its pharmaceutical effect on gastrointestinal function in rat model. Biotechol. Bioprocess Eng. 11, 240-244. https://doi.org/10.1007/BF02932037
  22. Yamamoto, R., Seki, M., Ouchi, K., Koyano, S., Nakazawa, Y., Nagatani, Y., and Sato, H. (1998) A rapid determination of allantoin by high-performance liquid chromatography using tris(hydroxyl-methyl)aminomethane-HCL buffer as a mobile phase. J. Pharmaceu. Soc. Jpn. 118, 310-316.
  23. Yeh, C. T. and Yen, G. C. (2005) Effct of vegetables on human phenolsulfotransferases in relation to their antioxidant activity and total phenolics. Free Radic. Res. 39, 893-904. https://doi.org/10.1080/10715760500150424
  24. Yin, J., Tezuka, Y., Kouda, K., Tran, Q. L., Miyahara, T., Chen, Y., and Kadota, S. (2004) Antiosteoporotic activity of the water extract of Dioscorea spongiosa. Biol. Pharm. Bull. 27, 583-586. https://doi.org/10.1248/bpb.27.583
  25. Yoo, E. J., Lim, H. S., Park, K. O., and Choi, M. R. (2005) Cytotoxic antioxidative, and ACE inhibiting activities of Dolsan leaf mustard juice (DLMJ) treated with lactic acid bacteria. Biotechnol. Bioprocess Eng. 10, 60-66. https://doi.org/10.1007/BF02931184
  26. Yoon, K. D., Yang, M. H., Chin, Y. W., Park, J. H., and Kim, J. (2008) Determination of allantoin in Dioscorea Rhizoma by high performance liquid chromatography using cyano columns. Natural Product Sci. 14, 254-259.
  27. Yoon, K. Y., Woodams, E. E., and Hang, Y. D. (2004) Probiotication of tomato juice by lactic acid bacteria. J. Microbiol. 42, 315-318.
  28. Zhao, G., Kan, J., Li, Z., and Chen, Z. (2005) Structural features and immunological activity of a polysaccharide from Dioscorea opposita Thunb. roots. Carbohydr. Polym. 61, 125-131. https://doi.org/10.1016/j.carbpol.2005.04.020

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