Safety and Health at Work

Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency (산업안전보건연구원)
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A Study of Micronucleus Induction with Methyl Formate and 2-Methylbutane in Bone Marrow Cells of Male ICR Mice

  • Kim, Soo-Jin (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA) ;
  • Rim, Kyung-Taek (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA) ;
  • Kang, Min-Gu (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA) ;
  • Kim, Jong-Kyu (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA) ;
  • Chung, Yong-Hyun (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA) ;
  • Yang, Jeong-Sun (Center for Chemicals Safety and Health, Occupational Safety and Health Research Institute, KOSHA)
  • Received : 2010.05.31
  • Accepted : 2010.07.13
  • Published : 2010.09.30
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Abstract

Objectives: We investigated the genotoxicity of two chemicals, methyl formate and 2-methylbutane, using male ICR mice bone marrow cells for the screening of micronucleus induction. Although these two chemicals have already been tested numerous times, a micronucleus test has not been conducted and the amounts used have recently been increased. Methods: 7 week male ICR mice were tested at dosages of 250, 500, and 1,000 mg/kg for methyl formate and 500, 1,000, and 2,000 mg/kg for 2-methlybutane, respectively. After 24 hours of oral administration with the two chemicals, the mice were sacrificed and their bone marrow cells were prepared for smearing slides. Results: As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocytes, all treated groups expressed no statistically significant increase of MNPCE compared to the negative control group. There were no clinical signs related with the oral exposure of these two chemicals. Conclusion: It was concluded that the two chemicals did not induce micronucleus in the bone marrow cells of ICR mice, and there was no direct proportion with dosage. These results indicate that the two chemicals have no mutagenic potential under each study condition.

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References

  1. O'Neil MJ. The Merck index: an encyclopedia of chemicals,drugs, and biologicals. 13th ed. Whitehouse Station (NJ):Merck Research Laboratories; 2001. 1084 p.
  2. National Institute for Occupational Safety and Health.NIOSH pocket guide to chemical hazards and other databases[CD-ROM]. Cincinnati (OH): U.S. Department of Health andHuman Services, Public Health Service, Centers for DiseaseControl and Prevention, National Institute for OccupationalSafety and Health; 2000. DHHS publication No.: (NIOSH) 2000-130.
  3. Lewis RJ Sr, Sax NI. Sax’s dangerous properties of industrialmaterials. 10th ed. New York (NY): John Wiley & Sons;2000. 4000 p.
  4. Lide DR. CRC handbook of chemistry and physics. 81st ed.Boca Raton (FL): CRC Press; 2000. p. 3-167.
  5. Spence AB, Colonna GR. Fire protection guide to hazardousmaterials. 13th ed. Quincy (MA): National Fire ProtectionAssociation; 2002. p. 49-99.
  6. Lewis RJ Sr, Sax NI. Sax’s dangerous properties of industrialmaterials. 9th ed. New York (NY): Van Nostrand Reinhold;1996. 1759 p.
  7. Browning E, Thurman RG, Kauffman FC. Ethel Browning’stoxicity and metabolism of industrial solvents. 2nd ed. NewYork (NY): Elsevier; 1992. 394 p.
  8. Electronic code of federal regulations (e-CFR) [Internet].Washington, DC: US Government Printing Office. 2010 –[cited 2010 May 14]. Available from: http://www.gpoaccess.gov/ecfr.
  9. American Conference of Governmental Industrial Hygienists.2008 TLVs and BEIs: based on the documentation of thethreshold limit values for chemical substances and physicalagents and biological exposure indices. Cincinnati (OH):American Conference of Governmental Industrial Hygienists;2008. 252 p.
  10. ESIS: European chemical Substances Information System.Methyl formate (107-31-3) [Internet]. Brussels (Belgium):European Commission Research Center. 2010 – [cited 2010May 4]. Available from: http://ecb.jrc.it/esis.
  11. American Conference of Governmental Industrial Hygienists.Documentation of threshold limit values for chemicalsubstances and physical agents and biological exposureindices for 2001. Cincinnati (OH): American Conference ofGovernmental Industrial Hygienists; 2001.
  12. High production volume (HPV) challenge. Robustsummaries and test plans [Internet]. Washington, DC: U.S.Environmental Protection Agency. c2010 – [cited 2010 May 14]. Available from: http://www.epa.gov/HPV/pubs/summaries/viewsrch.htm.
  13. Bingham E, Afffield M, Albert RE, Baxter CS, Bus JS. Patty’stoxicology. 5th ed. New York (NY): John Wiley & Sons;2001. p. 4-26.
  14. Isopentane [Internet]. [place unknown]: InternationalProgramme on Chemical Safety. 1996 - [cited 2010 May 14].Available from: http://www.inchem.org/documents/icsc/icsc/eics1153.htm.
  15. Lide DR. CRC handbook of chemistry and physics. 86th ed.Boca Raton (FL): CRC Press; 2005. p. 3-30.
  16. Hawley GG, Lewis RJ Sr. Hawley’s condensed chemicaldictionary. 14th ed. New York (NY): John Wiley & Sons;2001. 1223 p.
  17. ESIS: European chemical Substances Information System.2-Methylbutane (78-78-4) [Internet]. Brussels (Belgium):European Commission Research Center. 2009 – [cited 2010May 14]. Available from: http://ecb.jrc.it/esis.
  18. Scott D, Evans HJ. X-ray-induced chromosomal aberrationsin vicia faba: changes in response during the cell cycle. MutatRes 1967;4:579-99. https://doi.org/10.1016/0027-5107(67)90044-9
  19. Organization for Economic Co-operation and Development.Guidelines for testing of chemicals. Test No. 474: mammalianerythrocyte micronucleus test. Paris (France): Organizationfor Economic Co-operation and Development; 1997. p. 1-10.
  20. International Chemical Safety Cards (ICSCs). Aninternational programe on chemical safety project [Internet].Atlanta (GA): Centers for Disease Control and Prevention.c2010 - [cited 2010 May 14]. Available from: http//www.cdc.gov/niosh/ipcs/nicstart.html.
  21. Bingham E, Afffield M, Albert RE, Baxter CS, Bus JS. Patty’stoxicology. 5th ed. New York (NY): John Wiley & Sons;2001. p. 548-52.
  22. National occupational exposure survey conducted from 1981-1983 [Internet]. Atlanta (GA): Centers for Disease Controland Prevention. 2010 – [cited 2010 May 14]. Available from:http://www.cdc.gov/noes.
  23. Verschueren K. Handbook of environmental data on organicchemicals. 4th ed. New York (NY): John Wiley & Sons; 2001.p. 1340.
  24. Electronic code of federal regulations (e-CFR): 40 CFR716.120 [Internet]. Washington, DC: US GovernmentPrinting Office. 2010 – [cited 2010 May 14]. Availablefrom: http://edocket.access.gpo.gov/cfr_2005/julqtr/pdf/40cfr716.120.pdf.
  25. Knasmuller S, Nersesyan A, Misik M, Gerner C, MikulitsW, Ehrlich V, Hoelzl C, Szakmary A, Wagner KH. Use ofconventional and -omics based methods for health claims ofdietary antioxidants: a critical overview. Br J Nutr 2008;99 E Suppl 1:ES3-52.
  26. Kramer PJ. Genetic toxicology. J Pharm Pharmacol1998;50:395-405. https://doi.org/10.1111/j.2042-7158.1998.tb06879.x
  27. Collins AR. The comet assay for DNA damage and repair:principles, applications, and limitations. Mol Biotechnol2004;26:249-61. https://doi.org/10.1385/MB:26:3:249
  28. Hoelzl C, Knasmuller S, Misik M, Collins A, Dusinska M,Nersesyan A. Use of single cell gel electrophoresis assays forthe detection of DNA-protective effects of dietary factors inhumans: recent results and trends. Mutat Res 2009;681:68-79. https://doi.org/10.1016/j.mrrev.2008.07.004
  29. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A,Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Singlecell gel/comet assay: guidelines for in vitro and in vivo genetictoxicology testing. Environ Mol Mutagen 2000;35:206-21. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
  30. Nersesyan A, Hoelzl C, Ferk F, Misik M, Knasmueller S. Useof single cell gel electrophoresis assays in dietary interventiontrials. In: Dhawan A, Anderson D, editors. The comet assayin toxicology. Cambridge (UK): Royal Society of Chemistry; 2009.
  31. Fenech M, Neville S, Rinaldi J. Sex is an important variableaffecting spontaneous micronucleus frequency in cytokinesis-blockedlymphocytes. Mutat Res 1994;313:203-7. https://doi.org/10.1016/0165-1161(94)90050-7
  32. Vanparys P, Vermeiren F, Sysmans M, Temmerman R. Themicronucleus assay as a test for the detection of aneugenicactivity. Mutat Res 1990;244:95-103. https://doi.org/10.1016/0165-7992(90)90056-P
  33. Collins AR, Duthie SJ, Dobson VL. Direct enzymic detectionof endogenous oxidative base damage in human lymphocyteDNA. Carcinogenesis 1993;14:1733-5. https://doi.org/10.1093/carcin/14.9.1733
  34. Cavallo D, Ursini CL, Setini A, Chianese C, Piegari P,Perniconi B, Iavicoli S. Evaluation of oxidative damageand inhibition of DNA repair in an in vitro study of nickelexposure. Toxicol In Vitro 2003;17:603-7. https://doi.org/10.1016/S0887-2333(03)00138-3

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