Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Cell Growth and Antioxidant Activity on Onion Juice Fermentation by Using Lactobacillus plantarum as Animal Probiotics

가축용 생균제 Lactobacillus plantarum을 이용한 양파즙 발효의 균체성장과 항산화 활성

  • Chang, Woo-Kyung (Department of Animal Science and Environment, Konkuk University) ;
  • Cho, Sang-Buem (Department of Animal Science and Environment, Konkuk University) ;
  • Kim, Dong-Woon (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Suk (Department of Animal Science and Technology, Sunchon University) ;
  • Kim, Soo-Ki (Department of Animal Science and Environment, Konkuk University)
  • 장우경 (건국대학교 동물생산환경학) ;
  • 조상범 (건국대학교 동물생산환경학) ;
  • 김동운 (국립축산과학원) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 김수기 (건국대학교 동물생산환경학)
  • Received : 2010.11.04
  • Accepted : 2010.11.18
  • Published : 2010.11.30
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Abstract

This study was conducted to establish the optimal medium condition for the animal probiotic Lactobacillus plantarum by using onion juice. Cell yield and antioxidant activity increased in proportion to high additive levels of onion juice in medium. Onion juice, sucrose and yeast extract were selected as media ingredient factors and the effects of their mixed ratio in medium were evaluated. The full factorial design consisted of 24 experimental runs and was employed to estimate the main effects of the factors and their interactions. Significant positive effects on cell yield and antioxidant activity was shown with yeast extract and onion juice, respectively. Significant interaction was found only between sucrose and yeast extract in antioxidant activity. Finally, we selected an optimal medium that was composed of (g/l) onion juice, 600; sucrose, 15; yeast extract, 5. The efficiency of this optimum medium was estimated by using a 5 l jar fermenter. As a result, the maximum cell yield was $9.7\;{\log}_{10}$ (CFU/ml) at 12 hr. Cell yield at the end of incubation (20 hr) was $8.9\;{\log}_{10}$ (CFU/ml) and it was very similar with the predicted value, $9.0\;{\log}_{10}$ (CFU/ml). Antioxidant activity of culture was maintained at about 60~65% during all incubation time, resulting in a higher-than-predicted activity of 47.1%.

본 연구는 양파즙을 이용하여 가축 사료첨가제용 Lactobacillus plantarum의 배양 최적화를 위하여 수행되었다. 양파즙의 첨가수준이 균체성장과 항산화 활성에 미치는 영향을 평가하였을 때, 그 첨가수준이 증가할수록 모두 높아졌다. 양파즙, 설탕 그리고 yeast extract를 배지성분요인으로 선택하였고, 배지 내 성분요인들의 혼합비율에 의한 효과를 평가하였다. 24 개의 실험들로 구성된 완전 요인 배치법을 이용하여 요인들의 주효과와 상호작용을 조사하였다. 균체성장과 항산화 활성에 대한 유의성 있는 긍정적 주효과는 각각 yeast extract와 양파즙에서 나타났다. 유의적인 상호작용으로는 항산화 활성에 대하여 설탕와 yeast extract 사이에서 나타났다. 최종적으로 양파 600 g/l, 설탕 15 g/l 및 효모추출물 5 g/l의 최적 배지조성을 선발하였다. 얻어진 최적배지의 효율을 분석하기 위하여 5 l 발효기를 이용하여 평가한 결과 최대 균체 성장량은 배양 12시간째에 $9.7\;{\log}_{10}$ (CFU/ml)로 나타났다. 배양 마지막인 20시간 때에는 $8.9\;{\log}_{10}$ (CFU/ml)의 균체 성장량을 보였으며 이는 예측된 $9.01\;{\log}_{10}$ (CFU/ml)과 매우 유사한 결과였다. 배양액의 항산화 활성은 모든 배양 시간대에서 약 60~65%로 유지되었으며, 이것은 예측된 값(47.1%)보다 훨씬 우수하였다.

Keywords

References

  1. Beharka, A. A. and T. G. Nagaraja. 1998. Effect of Aspergillus oryzae extract alone or in combination with antimicrobial compounds on ruminal bacteria. J. Dairy Sci. 81, 1591-1598. https://doi.org/10.3168/jds.S0022-0302(98)75725-X
  2. Chang, J. Y. and H. C. Chang. 2010. Improvements in the quality and shelf life of kimchi by fermentation with the induced bacteriocin-producing strain, Leuconostoc citreum GJ7 as a starter. J. Food Sci. 75, M103-M110. https://doi.org/10.1111/j.1750-3841.2009.01486.x
  3. Davis, M. E., T. Parrott, D. C. Brown, B. Z. de Rodas, Z. B. Johnson, C. V. Maxwell, and T. Rehberger. 2008. Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs. J. Anim. Sci. 86, 1459-1467. https://doi.org/10.2527/jas.2007-0603
  4. Desnoyers, M., S. Giger-Reverdin, G. Bertin, C. Duvaux-Ponter, and D. Sauvant. 2009. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants. J. Dairy Sci. 92, 1620-1632. https://doi.org/10.3168/jds.2008-1414
  5. Elnima, E. I., S. A. Ahmed, A. G. Mekkawi, and J. S. Mossa. 1983. The antimicrobial activity of garlic and onion extracts. Pharmazie 38, 747-748.
  6. Gardner, N. J., T. Savard, P. Obermeier, G. Caldwell, and C. P. Champagne. 2001. Selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. Int. J. Food Microbiol. 64, 261-275. https://doi.org/10.1016/S0168-1605(00)00461-X
  7. Griffiths, G., L. Trueman, T. Crowther, B. Thomas, and B. Smith. 2002. Onions - a global benefit to health. Phytother. Res. 16, 603-615. https://doi.org/10.1002/ptr.1222
  8. Jang, J. R., K. K. Kim, and S. Y. Lim. 2008. Anticancer and antioxidant effects of solvent extracts from dried onion with different drying methods. J. Life Sci. 18, 1271-1277. https://doi.org/10.5352/JLS.2008.18.9.1271
  9. Jang, Y. D., H. K. Oh, L. G. Piao, H. D. Choi, J. H. Yun, and Y. Y. Kim. 2009. Evaluation of probiotics as an alternative to antibiotic on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. J. Anim. Sci. Technol. 51, 25-32. https://doi.org/10.5187/JAST.2009.51.1.025
  10. Jung, J. H., S. M. Hong, H. J. Kim, Q. W. Meng, and I. H. Kim. 2010. Effect of probiotics in diet on growth performance, nutrient digestibility, fecal microbial count, noxious gases emission from the feces, and blood profile in early-finishing pigs. J. Anim. Sci. Technol. 52, 23-28. https://doi.org/10.5187/JAST.2010.52.1.023
  11. Kim, D. W., J. H. Kim, G. H. Kang, H. K. Kang, S. J. Lee, W. J. Lee, and S. H. Kim. 2008. Study on intestinal viability and optimum feeding method of Lactobacillus in broiler chickens. J. Anim. Sci. Technol. 50, 807-818. https://doi.org/10.5187/JAST.2008.50.6.807
  12. Kim, H. S., S. G. Jeong, H. S. Chae, J. S. Ham, C. N. Ahn, and J. M. Lee. 2004. Antioxidant activity and tolerance to reactive oxygen species of Lactobacillus spp. J. Anim. Sci. Technol. 46, 1007-1012. https://doi.org/10.5187/JAST.2004.46.6.1007
  13. Kim, J. W., Y. S. Kim, and K. H. Kyung. 2004. Inhibitory activity of essential oils of garlic and onion against bacteria and yeasts. J. Food Prot. 67, 499-504.
  14. Kim, S. H., D. W. Kim, S. Y. Park, J. H. Kim, G. H. Kang, H. K. Kang, D. J. Yu, J. C. Na, and S. J. Lee. 2008. Effect of dietary Lactobacillus on growth performance, intestinal microflora, development of ileal villi, and intestinal mucosa in broiler chickens. J. Anim. Sci. Technol. 50, 667-676. https://doi.org/10.5187/JAST.2008.50.5.667
  15. Kim, Y. H., M. Y. Shon, and N. J. Sung. 2004. Antioxidant and antimutagenic activities of hot water extract from white and yellow onions after simulated gastric digestion. J. Life Sci. 14, 925-930. https://doi.org/10.5352/JLS.2004.14.6.925
  16. Kim, Y. J., S. J. Jang, J. M. Park, C. U. Kim, and Y. S. Park. 2010. Culture conditions of garlic resistant lactic acid bacteria for feed additives. Food Eng. Prog. 14, 65-74.
  17. Krehbiel, C. R., S. R. Rust, G. Zhang, and S. E. Gilliland. 2003. Bacterial direct-fed microbials in ruminant diets: Performance response and mode of action. J. Anim. Sci. 81(E.Suppl.2), E120-E132.
  18. Lee, S. M., W. Y. Park, and Y. J. Kim. 2010. Effect of dietary supplementation with Rosemary and ${\alpha}$-tocopherol acetate on performance and meat quality of chicken meat during refrigerated storage. Korean J. Food Sci. Ani. Resour. 30, 472-478. https://doi.org/10.5851/kosfa.2010.30.3.472
  19. Lessard, M., M. Dupuis, N. Gagnon, E. Nadeau, J. J. Matte, J. Goulet, and J. M. Fairbrother. 2009. Administration of Pediococcus acidilactici or Saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after Escherichia coli challenge. J. Anim. Sci. 87, 922-934.
  20. Park, Y. S., J. K. Jang, Y. J. Choi, M. Chung, H. Park, and K.-S. Shim. 2009. Lactic acid fermentation of onion juice supplemented with puffed red ginseng extract. Food Eng. Prog. 13, 16-23.
  21. Ramos, F. A., Y. Takaishi, M. Shirotori, Y. Kawaguchi, K. Tsuchiya, H. Shibata, T. Higuti, T. Tadokoro, and M. Takeuchi. 2006. Antibacterial and antioxidant activities of quercetin oxidation products from yellow onion (Allium cepa) skin. J. Agric. Food Chem. 54, 3551-3557. https://doi.org/10.1021/jf060251c
  22. Rentfrow, G., M. L. Linville, C. A. Stahl, K. C. Olson, and E. P. Berg. 2004. The effects of the antioxidant lipoic acid on beef longissimus bloom time. J. Anim. Sci. 82, 3034-3037.
  23. Rodriguez, H., J. A. Curiel, J. M. Landete, B. de las Rivas, F. L. de Felipe, C. Goez-Cordoves, J. M. Mancheno, and R. Munoz. 2009. Food phenolics and lactic acid bacteria. Int. J. Food Microbiol. 132, 79-90. https://doi.org/10.1016/j.ijfoodmicro.2009.03.025
  24. Shin, J. S., O. S. Lee, and Y. J. Jeong. 2002. Changes in the components of onion viegars by two stages fermentation. Korean J. Food Sci. Technol. 34, 1079-1084.
  25. Shon, M. Y., S. D. Choi, G. G. Kahng, S. H. Nam, and N. J. Sung. 2004. Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem. Toxicol. 42, 659-666. https://doi.org/10.1016/j.fct.2003.12.002
  26. Swyers, K. L., A. O. Burk, T. G. Hartsock, E. M. Ungerfeld, and J. L. Shelton. 2008. Effects of direct-fed microbial supplementation on digestibility and fermentation end-products in horses fed low- and high-starch concentrates. J. Anim. Sci. 86, 2596-2608. https://doi.org/10.2527/jas.2007-0608
  27. Toepfer-Berg, T. L., J. Escobar, W. G. Van Alstine, D. H. Baker, J. Salak-Johnson, and R. W. Johnson. 2004. Vitamin E supplementation does not mitigate the acute morbidity effects of porcine reproductive and respiratory syndrome virus in nursery pigs. J. Anim. Sci. 82, 1942-1951.
  28. Zohri, A. N., K. Abdel-Gawad, and S. Saber. 1995. Antibacterial, antidermatophytic and antitoxigenic activities of onion (Allium cepa L.) oil. Microbiol. Res. 150, 167-172. https://doi.org/10.1016/S0944-5013(11)80052-2

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