Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Physiological Activities of Hot Water Extracts from Ecklonia cava Kjellman

감태 열수 추출물의 생리활성

  • 조은경 (신라대학교 바이오식품소재학과) ;
  • 최영주 (신라대학교 식품영양학과)
  • Received : 2010.08.26
  • Accepted : 2010.10.05
  • Published : 2010.11.30
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Abstract

The biological activity of hot water extract from Ecklonia cava Kjellman (ECE) was investigated to assess antioxidative, anti-skin aging, and nitrite scavenging abilities, as well as alcohol metabolizing activities. Antioxidant activity of ECE was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity. DPPH radical scavenging activity and SOD-like activity of ECE increased in a remarkably dose-dependent manner, and were about 91.4% and 75% at 1 mg/ml, respectively. The xanthine oxidase inhibitory activity was indicated to be about 70% at 1 mg/ml of ECE. Nitrite scavenging ability of ECE showed to be 93.6% at 1 mg/ml and pH 1.2. The influence of ECE on alcohol metabolism was demonstrated through the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). The facilitating rate of ADH and ALDH activity by ECE was 167.2% and 334% at 10 mg/ml, respectively. In addition, tyrosinase and elastase inhibitory activities of ECE were 58% and 72% at 10 mg/ml, respectively. These results indicated that ECE has valuable biological attributes owing to its antioxidant, nitrite scavenging, alcohol metabolizing, and elastase and tyrosinase inhibitory activities.

해조류 감태의 기능성을 증명하기 위하여 열수 추출하여 여러 가지 생리활성에 대하여 조사하였다. 우선, 감태열수 추출물의 항산화능을 조사하기 위하여 DPPH radical 소거능, SOD 유사활성, XO 저해능을 측정하였다. 그 결과 감태 열수 추출물의 농도가 증가 할수록 높은 항산화능이 나타났으며 항산화제로 잘 알려져 있는 BHA, VitC, catechin의 활성보다 높거나 유사하게 보였다. 아질산염 소거능 측정 실험에서 positive control인 VitC와 감태 열수 추출물은 시료농도가 증가할수록 pH가 낮을수록 증가하였다. 특히, pH 1.2에서 감태 열수 추출물의 경우 VitC 보다 약간 높은 아질산염 소거능을 나타내었다. 감태 열수 추출물의 숙취해소 효능은 ADH와 ALDH 활성증진에 감태 열수 추출물이 미치는 영향을 조사함으로써 증명하고자 하였다. 그 결과, 감태 열수 추출물은 알콜과 acetaldehyde 분해능을 모두 가지고 있을 뿐만 아니라 acetaldehyde의 분해능은 상당히 높게 나타났다. 감태 열수 추출물의 미용효능은 tyrosinase와 elastaase 억제 효과를 분석함으로써 증명하고자 하였다. Tyrosinase 저해능 분석에서 positive control로 사용한 kojic acid는 0.1 mg/ml의 농도에서 65%의 tyrosinase 억제효과를 나타낸 반면, 감태 열수 추출물의 경우 10 mg/ml의 농도에서 58%의 저해효능을 보였다. 비록, 그 효능이 충분히 높지는 않지만 감태 열수 추출물의 농도가 증가함에 따라서 tyrosinase 저해활성이 증가하므로 충분한 미백효능을 기대할 수 있을 것으로 판단된다. Elastase 억제 효능 분석에서는 감태 열수 추출물 0.5 mg/ml에서 38%, 1 mg/ml에서 44%의 저해활성이 나타났다. Positive control로 elastase 저해 활성 효과가 알려진 VitC는 1 mg/ml의 농도에서 36%의 저해활성이 나타났고 elastase 활성저해제인 oleanolic acid는 농도 0.5 mg/ml에서 elastase를 32% 정도 저해하는 것으로 알려져 있으므로 주름 예방 효능에 있어서 감태 열수 추출물의 우수한 기능성이 예상된다.

Keywords

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