생명과학회지 (Journal of Life Science)

  • 제20권11호
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  • Pages.1667-1674
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Cadmium을 처리하여 기내 배양한 담배 식물의 생장, 엽록소 함량 및 rubisco와 rubisco activase의 함량과 활성에 미치는 질산염의 영향

Influence of Nitrate on Growth, Chlorophyll Content, Content and Activity of Rubisco and Rubisco Activase of Tobacco Plant Treated with Cadmium in vitro

  • 투고 : 2010.08.25
  • 심사 : 2010.10.28
  • 발행 : 2010.11.30
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초록

Cd을 처리하여 기내 배양한 담배 식물의 생장과 엽록소 함량, rubisco와 rubisco activase의 함량과 활성에 미치는 질산염의 영향을 연구하였다. Cd에 의해 억제되었던 담배 식물의 생장과 엽록소의 함량은 질산염에 의해 회복되었으며, 80 mM 질산염에서 회복 효과가 가장 높았다. 80 mM 질산염에서의 rubisco의 함량이 타 농도에서 보다 현저하게 증가하였으며, rubisco의 활성 또한 rubisco의 함량과 같은 변화를 보였다. 이 결과들은 Cd에 의해 감소된 rubisco의 함량과 활성이 질산염에 의해 회복되었음을 의미한다. SDS-PAGE의 결과, 55 kD와 15 kD의 large subunit와 small subunit의 강도는 타 농도에서 보다 80 mM 질산염에서 현저하게 증가하였다. Rubisco activase의 함량과 활성을 측정한 결과, 80 mM 질산염에서의 rubisco activase의 함량은 타 농도에서 보다 현저하였으며, 이의 활성은 함량과 같은 양상을 나타내어, rubisco에 대한 질산염에 의한 회복 현상이 rubisco activase와 관련 있음을 추측하게 한다.

Influence of nitrate on growth, chlorophyll content, content and activity of rubisco and rubisco activase of tobacco plant cultured on MS medium treated with cadmium in vitro was studied. In vitro growth and chlorophyll content reduced at 0.2 mM Cd was recovered by nitrate and this recovery was most significant at 80 mM nitrate. Rubisco content at 80 mM nitrate was more increased compared to that at other concentrations. A similar change was also shown in rubisco activity. These resultsindicate that the activation and induction of rubisco reduced by Cd were recovered by nitrate. The degree of intensity of 55 and 15 kD polypeptides identified as the large and small subunits of rubisco by SDS-PAGE analysis at 80 mM nitrate was significantly higher than that at other concentrations. The content and activity of rubisco activase at 80 mM nitrate was significantly increased than that at other concentrations. These data suggest that the recovery effects of rubisco by nitrate may be associated with rubisco activase.

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