Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antiinflammatory Effect of Bioactive Compounds from Geranium nepalense (Notes)

현초에서 분리한 생리활성물질의 항염증효과 (단보)

  • Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University) ;
  • Kim, Eui-Sung (Eco-Friendly Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Young-Hang (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University) ;
  • Yu, Byung-Soo (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University) ;
  • Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Sciences, Wonkwang University)
  • 김보미 (원광대학교 자연과학대학 나노생명화학부) ;
  • 김의성 (한국생명공학연구원 전북분원) ;
  • 이영행 (원광대학교 자연과학대학 나노생명화학부) ;
  • 유병수 (원광대학교 자연과학대학 나노생명화학부) ;
  • 채규윤 (원광대학교 자연과학대학 나노생명화학부)
  • Received : 2010.10.27
  • Accepted : 2010.11.26
  • Published : 2010.12.30
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Abstract

The Geranium nepalense has been used traditionally for treatment of various diseases. However, the molecular studies on the effect of Geranium nepalense have not been carried out. In the present study, Quercetin, quercitrin, and afzelin were isolated from the methanol extract of Geranium nepalense were tested for their anti-inflammatory effect. The anti-inflammatory effect of the compounds was studied in lipopolysaccharide(lps)-treated mouse macrophage cells, RAW 264.7. RAW 264.7 cells were pre-incubated with isolated compounds(0, 5, 10, 20, 40, $50\;{\mu}g/ml$) for 4h and treated with $1\;{\mu}g/ml$ lps for 18h, and then the anti-inflammatory effects of compounds were determined. The results are as follows: Quercetin at various concentration inhibited the viability of Raw 264.7 from 7% to 45%, quercitrin from 25% to 80%, and afzelin from 13% to 52%. Isolated compounds showed a significant decrease in iNOS (inducible nitric oxide synthase) and COX-2 (cyclooxygenase-2). These results suggest that these compounds can be used as stable anti-inflammatory materials.

Keywords

References

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