Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Identification of 'Chunpoong' among Panax ginseng Cultivars Using Real Time PCR and SNP Marker

  • Sun, Hua (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Ok-Ran (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Jeong, Seok-Kyu (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kwon, Woo-Saeng (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Se-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
  • Published : 2010.03.31
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Abstract

The common DNA extraction methods are indispensable for genotyping by molecular marker analysis. However, genotyping a large number of plants is painstaking. A modified 'NaOH-Tris' method used in this study reduces the extraction time while keeping the cost low and avoiding the use of hazardous chemicals. The endpoint analysis by realtime PCR tends to be fast and effective for the development of SNP markers linked to the 'Chunpoong' cultivar of Panax ginseng. The 'Chunpoong' marker was developed by a major latex-like protein gene sequence. From our results, we suggest that this method is successful in distinguishing 'Chunpoong' from a large number of ginseng cultivars.

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References

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