Asian-Australasian Journal of Animal Sciences

  • 제23권3호
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  • Pages.318-325
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  • 2010
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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The Effects of Different Concentrations of Glycine and Cysteine on the Freezability of Moghani Ram Spermatozoa

  • Khalili, B. (Jafar-Abad Livestock Central Research Institute, Ministry of Agriculture) ;
  • Jafaroghli, M. (College of Agriculture, University of Payam-e- Noor) ;
  • Farshad, Abbas (Department of Animal Science, College of Agriculture, University of Kurdistan) ;
  • Paresh-Khiavi, M. (Jafar-Abad Livestock Central Research Institute, Ministry of Agriculture)
  • 투고 : 2009.07.19
  • Accepted : 2009.10.30
  • Published : 2010.03.01
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Abstract

Two experiments were designed to evaluate the effects of the amino acids glycine and cysteine on cryopreservation of ram spermatozoa. After primary evaluation of collected ejaculates, the semen samples were pooled and diluted 1:4 before cooling (experiment 1) and freezing (experiment 2) with Tris-Citrate-Fructose-Yolk (TCFY) extender supplemented with different concentrations of glycine and cysteine (5, 10, 15 and 20 mM). As the control, semen was diluted and frozen in the extender without amino acids. Motility, viability and membrane integrity were assessed as the parameters for semen quality in the first experiment. In the second experiment, motility, progressive motility, viability, membranes and acrosome integrity were evaluated after the freezing-thawing process. The results of the first experiment indicated that the addition of 10 and 15 mM cysteine compared to the control (basic) extender significantly increased (p<0.01) the motility, viability and membrane integrity of spermatozoa after cooling. However, further increasing these amino acids up to 20 mM had a significant negative effect (p<0.05). Our results showed no significant differences (p>0.05) between 5 mM glycine compared to 5 mM cysteine and between 20 mM glycine and 20 mM cysteine. The results of experiment 2 showed that the amino acids significantly improved post-thaw motility, progressive motility, viability, membranes and acrosome integrity of ram spermatozoa. These positive effects were observed at concentrations between 5 to 15 mM of glycine and cysteine, with the best results at 15 mM. Further increasing of amino acid concentrations significantly decreased the post-thaw characteristics of spermatozoa, but the results showed that cysteine was better than glycine and control extenders. The data indicated that addition of glycine or cysteine to the freezing extender can be recommended for cryopreservation of ram spermatozoa. However, further studies are still needed to determine the effect of such addition on fertility in farm animals.

Keywords

References

  1. Aitken, R. J. and H. Fisher. 1994. Reative oxygen species generation and human spermatozoa: the balance of benefit and risk. Bio-assays. 16:259-267
  2. Ali Al Ahmad, M. Z., G. Chatagnon, L. Amirat-Briand, M. Moussa, D. Tainturier, M. Anton and F. Fieni. 2008. Use of glutamine and low density lipoproteins isolated from egg yolk to improve buck semen freezing. Reprod. Domest. Anim. 43:429-436 https://doi.org/10.1111/j.1439-0531.2007.00930.x
  3. Alvarez, J. G. and B. T. Storey. 1983. Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol. Reprod. 29:548-555 https://doi.org/10.1095/biolreprod29.3.548
  4. Anchordoguy, T., J. F. Carpenter, S. H. Loomis and J. H. Crowe. 1988. Mechanisms of interaction of amino acids with phospholipid bilayers during freezing. Biochim. Biophys. Acta. 946:299-306 https://doi.org/10.1016/0005-2736(88)90405-1
  5. Atessahin, A., M. N. Bucak, P. B. Tuncer and M. Kizil. 2008. Effect of antioxidant additives on microscopic and oxidative parameters of Angora goat semen following the freeze-thawing process. Small Rumin. Res. 77:38-44 https://doi.org/10.1016/j.smallrumres.2008.03.002
  6. Bailey, J. L., J. F. Bilodeau and N. Cormier. 2000. Semen cryopreservation in domestics animals: a damaging and capaciting phenomenon. J. Androl. 21:1-7
  7. Baumber, J., B. A. Ball, C. G. Gravance, V. Medina and M. C. G. Davies-Morel. 2000. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential and membrane lipid peroxidation. J. Androl. 21:895-902
  8. Billard, R. and Y. Menezo. 1984. The amino acids composition of rainbow trout seminal fluid and blood plasma: a comparison with carp. Aquaculture 41:255-258 https://doi.org/10.1016/0044-8486(84)90288-6
  9. Bilodeau, J. F., S. Blanchette, I.C. Gagnon and M. A. Sirard. 2001. Thiols prevent $H_2O_2$ - mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 56:275-286 https://doi.org/10.1016/S0093-691X(01)00562-3
  10. Bucak, M. N., A. Atessahin and Y. Abdurrauf. 2008. Effect of antioxidants and oxidative stress parameters on ram after the freeze-thawing process. Small Rumin. Res. 75:128-134 https://doi.org/10.1016/j.smallrumres.2007.09.002
  11. Chu, T. M., D. Aspinall and L. G. Paleg. 1974. Stress metabolism: Part 6. Temperature stress and the accumulation of proline in barley and radish. Aust. J. Plant Physiol. 1:87-97 https://doi.org/10.1071/PP9740087
  12. Curry, M. R., J. D. Millar and P. F. Watson. 1994. Calculated optimal cooling rates for ram and human sperm cryopreservation fail to confirm with empirical observations. Biol. Reprod. 51:1014-1021 https://doi.org/10.1095/biolreprod51.5.1014
  13. Darin-Bennett, A. and I. G. White, 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology 14: 466-470 https://doi.org/10.1016/0011-2240(77)90008-6
  14. Dhami, A. J. and K. L. Shani. 1994. Evaluation of different cooling rates, equilibration periods and diluents for effects on deep-freezing, enzyme leakage and fertility of taurine bull spermatozoa. Theriogenology 40:1269-1280 https://doi.org/10.1016/0093-691X(93)90297-I
  15. Dhami, A. J., K. L.Shani and G. Mohan. 1995. Role of prefreeze cooling/holding times, dilutors and thaw rates in improving freezability and post thaw survival of bovine spermatozoa. Ind. Anim. Reprod. 16:117-120
  16. Drobnis, E. Z., L. M. Crowe and T. Berger. 1993. Cold shock damage is due to lipid phase-transitions in cell membranes:a demonstration using sperm as a model. J. Exp. Zool. 265:432-437 https://doi.org/10.1002/jez.1402650413
  17. Eiman, M.-E. A. and T. Terada. 2004. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol. Reprod. 69:1245-1250 https://doi.org/10.1095/biolreprod.103.017889
  18. Evans, G. and W. M. S. Maxwell. 1987. Salamon's artificial insemination of sheep and goats. University Press, Sydney, NSW, Australia
  19. Farshad, A., B. Khalili and P. Fazeli. 2009. The effect of different concentrations of glycerol and DMSO on viability of Markhoz goat spermatozoa during different freezing temperatures steps. Pak. J. Biol. Sci. 12:239-245 https://doi.org/10.3923/pjbs.2009.239.245
  20. Fiser, P. S. and R. W. Fairful. 1989. The effect of glycerol related osmotic changes on post thaw motility and acrosomal integrity of ram spermatozoa. Cryobiology 26:64-69 https://doi.org/10.1016/0011-2240(89)90033-3
  21. Flipse, R. J. and J. Q. Almquist. 1955. Effect of milk-glycine and egg yolk-glycine diluents on the survival of bovine spermatozoa. J. Anim. Sci. 14:1182
  22. Flipse, R. J. and J. Q. Almquist. 1956. Diluters for bovine semen. IX. Motility of bovine spermatozoa in milk-glycine and egg yolk-glycine diluents with and without glycerol, in: J. Pennsylv. Agric. Exp. Station. 1690-1696
  23. Foote, R. H. 1970. Fertility of bull semen at high extension rates in Tris Buffered Extenders. J. Dairy Sci. 53:1475-1477 https://doi.org/10.3168/jds.S0022-0302(70)86417-7
  24. Gadella, B. M., R. Rathi, J. F. H. M. Brouwers, T. A. E. Stout and B. Colenbrander. 2001. Capacitation and the acrosome reaction in equine sperm. Anim. Reprod. Sci. 68:249-265 https://doi.org/10.1016/S0378-4320(01)00161-0
  25. Hammerstedt, R. H., J. K. Graham and J. P. Nolan. 1990. Cryopreservation of mammalian sperm: what we ask them to survive. J. Androl. 11:73-88
  26. He, S. and L. C. Woods. 2003. Effect of glycine and alanine on short term storage and cryopreservation of striped bass (Morone saxatilis) spermatozoa. Cryobiology 46:17-25 https://doi.org/10.1016/S0011-2240(02)00159-1
  27. Iqbal, N. and A. G. Hunter. 1991. Comparison of bovine sperm capacitations systems. J. Dairy Sci. 74:228
  28. Khlifaouia, M., I. Battuta, J. F. Bruyasa, G. Chatagnona, A. Trimecheb and D. Tainturiera. 2005. Effects of glutamine on post-thaw motility of stallion spermatozoa: an approach of the mechanism of action at spermatozoa level. Theriogenology 63:138-149 https://doi.org/10.1016/j.theriogenology.2004.04.012
  29. Kruuv, J. and D. J. Glofcheski. 1992. Protective effect of amino acids against freeze-thaw damage in mammalian cells. Cryobiology 29:291-295 https://doi.org/10.1016/0011-2240(92)90028-Z
  30. Kumar, S., J. D. Millar and P. F Watson. 2003. The effect of cooling rate on the survival of cryopreserved bull, ram and boar spermatozoa: a comparison of two controlled-rate cooling machines. Cryobiology 46:246-253 https://doi.org/10.1016/S0011-2240(03)00040-3
  31. Kundu, C. N., K. Das and G. C. Majumder. 2001. Effect of amino acids on cauda epididymal sperm cryopreservation using a chemically defined model system. Cryobiology 41:21-27 https://doi.org/10.1006/cryo.2001.2296
  32. Lalonde, R., J. Lepock and J. Kruuv. 1991. Site of freeze-thaw damage and cryopreservation by amino acids of the calcium ATPase of the sarcoplasmic reticulum. Biochem. Biophys. Acta. 1079:128-138 https://doi.org/10.1016/0167-4838(91)90117-I
  33. Lahnsteiner, F., T. Weismann and R. A. Patzner. 1992. Fine structural changes in spermatozoa of the grayling, Thymallus thymallus (Pisces:Teleostei), during routine cryopreservation. Aquaculture 103:73-84 https://doi.org/10.1016/0044-8486(92)90280-X
  34. Li, Y., W. Si, X. Zhang, A. Dinnyes and W. Ji. 2003. Effect of amino acids on cryopreservation of cynmolgus monkey (macaca fascicularis) sperm. Am. J. Primatol. 59:159-165 https://doi.org/10.1002/ajp.10073
  35. Matsuoka, T., H. Imai, H. Kohno and Y. Fukui. 2006. Effect of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J. Reprod. Dev. 52:675-683 https://doi.org/10.1262/jrd.18033
  36. Moore, A. I., E. L. Squires and J. K. Graham. 2005. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology 51:241-249 https://doi.org/10.1016/j.cryobiol.2005.07.004
  37. Morris, G. I. and A. Clarke. 1987. Cell at low temperatures. In: The effects of low temperatures on biological systems (Ed. B. W. W. Grout and G. J. Morris). London, Edward Arnold, 72-119
  38. Morris, G. I., G. I. Morris and A. Clarke. 1981. Effects of low temperature on biological membranes. New York, Academic Press, 241-377
  39. Noguchi, S. and J. Matsmuto. 1971. Studies on the control of the denaturation of the fish muscle proteins during frozen storage II. Preventing effect of amino acids and related compounds. Bull. Jap. Soc. Sci. Fish. 37:1115-1122 https://doi.org/10.2331/suisan.37.1115
  40. Purdy, P. H. 2006. A review on goat sperm cryopreservation. Small Rumin. Res. 63:215-225 https://doi.org/10.1016/j.smallrumres.2005.02.015
  41. Purdy, P. H., M. H. Fox and J. K. Graham. 2005. The fluidity of chinese hamster ovary cell and bull sperm membranes after cholesterol addition. Cryobiology 51:102-112 https://doi.org/10.1016/j.cryobiol.2005.05.004
  42. Revell, S. G. and R. A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36:77-86 https://doi.org/10.1016/0378-4320(94)90055-8
  43. Roy, A. and M. W. H. Bishop. 1954. Effect of glycine on the survival of bull spermatozoa in vitro. Nature 174:746-747 https://doi.org/10.1038/174746a0
  44. Rudolph, A. S. and J. H. Crowe. 1985. Membraned stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. Cryobiology 22:367-377 https://doi.org/10.1016/0011-2240(85)90184-1
  45. Salamon, S. and W. M. Maxwell. 2000. Storage of ram semen. Anim. Reprod. Sci. 62:77-111 https://doi.org/10.1016/S0378-4320(00)00155-X
  46. SAS, 1996. SAS/STAT Software: Changes and Enhancements through Release 6.12. SAS Institute Inc., Cary, NC, USA
  47. Scanchez-Partidata, L. G., W. M. C. Maxwell, L. G. Paleg and B. P. Setchell. 1992. Proline and glycine betaine in cryoprotective diluents for ram spermatozoa. Reprod. Fertil. Dev. 4:113-118 https://doi.org/10.1071/RD9920113
  48. Sheshtawy, R. I., G. A. El-Sisy and W. S. El-Nattat. 2008. Use of selected amino acids to improve buffalo bull semen cryopreservation. Global Vet. 2:146-150
  49. Sinha, M. P., A. K. Sinha, B. K. Singh and P. L. Prasad. 1996. The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Theriogenology 41:237-243
  50. Szczesniak-Fabianczyk, B., M. Bochenek, Z. Smorag and M. A. Silvestre. 2006. Effect of antioxidants added to boar semen extender on the semen survival time and sperm chromatin structure. Reprod. Biol. 3:81-87
  51. Trimèche, A., J. M. Yvon, M. Vidament, E. Palmer and M. Magistrini. 1999. Effects of glutamine, proline, histidine and betaine on post-thaw motility of stallion spermatozoa, Theriogenology 52:181-191 https://doi.org/10.1016/S0093-691X(99)00120-X
  52. Uysal, O. and M. N. Bucak. 2007. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet. Brno 76:383-390 https://doi.org/10.2754/avb200776030383
  53. Watson, P. F. 1981. The role of lipid and protein in the proteiction of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. J. Reprod. Fertil. 62:483-492 https://doi.org/10.1530/jrf.0.0620483
  54. Watson, P. F. 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 61:481-492 https://doi.org/10.1016/S0378-4320(00)00099-3
  55. Watson, P. F. and I. C. A. Martin. 1975. Effects of egg yolk, glycerol and the freezing rate on the viability and acrosomal structures of frozen ram spermatozoa. Aust. J. Biol. Sci. 28: 153-159
  56. Weitze, K. F. 1977. Untersuchungen zur Tiefgefriekonservierung von Kaninchensperma. Habil- Schr., Tieraerztl. Hochs. Hannover, Germany

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