Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Quercetin on the Activity and mRNA Expression of Antioxidant Enzymes and Physiological Responses in Olive Flounder (Paralichthys olivaceus) Exposed to Cadmium

  • Shin, H.S. (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Yoo, J.H. (Jeilfeed company Ltd.) ;
  • Min, T.S. (Division of Medical and Pharceutical Science, National Research Foundation of Korea) ;
  • Lee, J. (Department of Marine Life Sciences, Jeju National University) ;
  • Choi, C.Y. (Division of Marine Environment and Bioscience, Korea Maritime University)
  • Received : 2010.01.05
  • Accepted : 2010.03.24
  • Published : 2010.06.01
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Abstract

We investigated the antioxidant efficacy of quercetin (0% Diet 1, 0.25% Diet 2, and 0.5% Diet 3) pretreatment for 30 and 60 days in response to cadmium (Cd) toxicity in the olive flounder, and measured the plasma lysozyme activity to understand the immune effects of quercetin. The lysozyme activity with Diets 2 and 3 was higher than with Diet 1. Based on this result, to examine the immune ability and antioxidant role of quercetin, we exposed olive flounder fed quercetin to Cd and then measured the expression and activity of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) and lipid peroxidation (LPO). With Diets 2 and 3, the expression and activity of antioxidant enzymes and the $H_2O_2$ concentration were lower than with Diet 1. In addition, the LPO levels were lower than with Diet 1, which protected the cell membrane. Therefore, quercetin removed the reactive oxygen species (ROS) produced by Cd, indicating that quercetin has antioxidant ability. In addition to its antioxidant ability, quercetin has immune effects.

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References

  1. Ai, Q. H., K. S. Mai, C. X. Zhang, W. Xu, Q. Y. Duan, B. P. Tan and Z. G. Liufu. 2004. Effects of dietary vitamin C on growth and immune response of Japanese seabass, Lateolabrax japonicus. Aquaculture 242:489-500 https://doi.org/10.1016/j.aquaculture.2004.08.016
  2. Al-Jassabi, S. 2005. Biochemical studies on the role of lycopene in the protection of mice against microcystin toxicity. J. Chem. Ecol. 21:143-148 https://doi.org/10.1080/02757540500093089
  3. Atencio, L., I. Moreno, Jos $\'{A}$, A. I. Prieto, R. Moyano, A. Blanco and A. M. Cameán. 2009. Effects of dietary selenium on the oxidative stress and pathological changes in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom. Toxicon 53:269-282 https://doi.org/10.1016/j.toxicon.2008.11.011
  4. Basha Siraj, P. and A. Rani Usha. 2003. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicol. Environ. Saf. 56:218-221 https://doi.org/10.1016/S0147-6513(03)00028-9
  5. Bols, N. C., J. L. Brubacher, R. C. Ganassin and L. E. J. Lee. 2001. Ecotoxicology and innate immunity in fish. Dev. Comp. Immunol. 25:853-873 https://doi.org/10.1016/S0145-305X(01)00040-4
  6. Bors, W. and M. Saran. 1987. Radical scavenging by flavonoid antioxidants. Free Radic. Res. Commun. 2:289-294 https://doi.org/10.3109/10715768709065294
  7. Chen, J., W. Q. Zhou, L. Feng, Y. Liu and J. Jiang. 2009. Effects of glutamine on hydrogen peroxide-induces oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var. Jian). Aquaculture 288:285-289 https://doi.org/10.1016/j.aquaculture.2008.10.053
  8. da-Silva, W. S., J. W. Harney, B. W. Kim, J. Li, S. D. Bianco and A. Crescenzi. 2007. The small polyphenolic molecule kaempferol increases cellular energy expenditure and thyroid hormone activation. Diabetes 56:767-776 https://doi.org/10.2337/db06-1488
  9. Dautremepuits, C., S. Betoulle, S. Paris-Palacios and G. Vernet. 2004. Humoral immune factors modulated by copper and chitosan in healthy or parasitized carp (Cyprinus carpio L.) by ptychobothrium sp. (Cestoda). Aquat. Toxicol. 68:325-338 https://doi.org/10.1016/j.aquatox.2004.04.003
  10. Eo, J. and K. J. Lee. 2008. Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish Shellfish Immunol. 25:611-616 https://doi.org/10.1016/j.fsi.2008.08.009
  11. Esterbauer, H., R. J. Schaur and H. Zoliner. 1991. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic. Biol. Med. 11:81-128 https://doi.org/10.1016/0891-5849(91)90192-6
  12. Frankle, E. N., H. Kanner, J. B. German, E. Parks and J. E. Kinsella. 1993. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 341:454-457 https://doi.org/10.1016/0140-6736(93)90206-V
  13. Gouillou-Coustans, M. F., P. Bergot and S. J. Kaushik. 1998. Dietary ascorbic acid needs of common carp (Cyprinus carpio) larvae. Aquaculture 161:453-461 https://doi.org/10.1016/S0044-8486(97)00292-5
  14. Hansen, B. H., S. R${\o}$mma, ${\O}$. A. Garmo, P. A. Olsvik and R. A. Anderson. 2006. Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Comp. Biochem. Physiol. C 143:263-274 https://doi.org/10.1016/j.cbpc.2006.02.010
  15. Hiratsuka, S., K. Ishihara, T. Kitagawa, S. Wada and H. Yokogoshi. 2008. Effect of dietary docosahexaenoic acid connecting phospholipids on the lipid peroxidation of the brain in mice. J. Nutr. Sci. Vitaminol. 54:501-506 https://doi.org/10.3177/jnsv.54.501
  16. Jayaraj, R., U. Deb, A. S. B. Bhaskar, G. B. K. S. Prasad and P. V. Lakshmana Rao. 2007. Hepatoprotective efficacy of certain flavonoids against microcystin induces toxicity in mice. Environ. Toxicol. 22:472-479 https://doi.org/10.1002/tox.20283
  17. Kang. S-K., Y-D. Kim, K-H. Hyun, Y-W. Kim, J-S. Seo and Y-K. Park. 1998. Development of separating techniques on quercetin-related substances in onion (Allium cepa L.) 2. Optimal extracting condition of quercetin-related substances in onion. J. Korean Soc. Food Sci. Nutr. 27:687-692
  18. Kinnula, V. L., J. D. Crapo and K. O. Raivio. 1995. Generation and disposal of reactive oxygen metabolites in the lung. Lab. Invest. 73:3-19
  19. Merchie, G., P. Lavens, J. Verrth, F. Ollevier, H. Nelis, A. De Leenheer, V. Storch and P. Sorgeloos. 1997. The effect of supplemental ascorbic acid in enriched live food for Clarias gariepinus larvae at start feeding. Aquaculture 151:245-258 https://doi.org/10.1016/S0044-8486(96)01505-0
  20. Monteiro, D. A., F. T. Rantin and A. L. Kalinin. 2009. The effects of selenium on oxidative stress biomarkers in the freshwater characid fish matrinxã (Brycon cephalus) (Günther, 1869) exposed to organophosphate insecticide Folisuper 600 BR (methyl parathion). Comp. Biochem. Physiol. C 149:40-49 https://doi.org/10.1016/j.cbpc.2008.06.012
  21. Nouroozzadeh, J., J. Tajaddinisarmadi and S. P. Wolff. 1994. Measurement of plasma hydroperoxide concentrations by ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Anal. Biochem. 200:403-409
  22. Ortuno, J., M. A. Esteban and J. Meseguer. 1999. Effect of high dietary intake of vitamin C on non-specific immune response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol. 9:429-443 https://doi.org/10.1006/fsim.1998.0201
  23. Pinho, G. L. L., C. Moura da Rosa, F. E. Maciel, A. Bianchini, J. S. Yunes, L. A. O. Proenca and M. J. Monserrat. 2005. Antioxidant responses after microcystin exposure in gills of an estuarine crab species pre-treated with vitamin E. Ecotoxicol. Environ. Saf. 61:361-365 https://doi.org/10.1016/j.ecoenv.2004.12.014
  24. Prieto, A. I., A. Jos, S. Pichardo, I. Moreno and A. M. Came$\'{a}$n. 2008. Protective role of vitamin E on the microcystin-induces oxidative stress in tilapia fish (Oreochromis niloticus). Environ. Toxicol. Chem. 27:1152-1159 https://doi.org/10.1897/07-496.1
  25. Qinghui, A., K. Mai, C. Zhang, W. Xu, Q. Duan, B. Tan and Z. Liufu. 2004. Effects of dietary vitamin C on growth and immune response of Japanese seabass, Lateolabrax japonicus. Aquaculture 242:489-500 https://doi.org/10.1016/j.aquaculture.2004.08.016
  26. Scalbert, A. and G. Williamson. 2000. Dietary intake and bioavailability of polyphenols. J. Nutr. 130:2073S-2085S
  27. Storey, K. B. 1996. Oxidative stress: animal adaptations in nature. Braz. J. Med. Biol. Res. 29:1715-1733
  28. Trenzado, C. E., A. E. Morales, J. M. Palma and M. Higuera. 2009. Blood antioxidant defenses and hematological adjustments in crowded/uncrowded rainbow trout (Oncorhynchus mykiss) fed on diets with different levels of antioxidant vitamins and HUFA. Comp. Biochem. Physiol. C 149:440-447 https://doi.org/10.1016/j.cbpc.2008.10.105
  29. Valavanidis, A., T. Vlahogianni, M. Dassenakis and M. Scoullos. 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicol. Environ. Saf. 64:178-189 https://doi.org/10.1016/j.ecoenv.2005.03.013
  30. Veliogue, Y. S. and G. Mazza. 1991. Characterization of flavonoids in pestals of Rosa damascene by HPLC and spectral analysis. J. Agric. Food Chem. 39:463-467 https://doi.org/10.1021/jf00003a007
  31. Wang, W. and A. Li. 1996. Influences of LAPP on the growth, resistance to low oxygen content and immunoresistance of Penaeus chinensis. Trans. Oceanol. Limnol. 1:42-49
  32. Weng, D., Y. Lu, Y. Wei, Y. Liu and P. Shen. 2007. The role of ROS in microcystin-LR-induced hepatocyte apoptosis and liver injury in mice. Toxicology 232:15-23 https://doi.org/10.1016/j.tox.2006.12.010
  33. Wheeler, C. R., J. A. Salzman and N. M. Elsayed. 1990. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal. Biochem. 184:193-199 https://doi.org/10.1016/0003-2697(90)90668-Y
  34. Xu, C., W. Shu, Z. Qui, J. Chen, Q. Zhao and J. Cao. 2007. Protective effects of green tea polyphenols against subacute hepatotoxicity induced by microcystin-LR in mice. Environ. Toxicol. Pharmacol. 24:140-148 https://doi.org/10.1016/j.etap.2007.04.004
  35. Zheng, Z. L., J. Y. W. Tan, H. Y. Liu, X. H. Zhou, X. Xiang and K. Y. Wang. 2009. Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth, antioxidant effect and resistance against Aeromonas hydrophila in channel catfish (Ictalurus punctatus). Aquaculture 292:214-218 https://doi.org/10.1016/j.aquaculture.2009.04.025

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