한국어류학회지 (Korean Journal of Ichthyology)

  • 제22권3호
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  • Pages.201-205
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  • 2010
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  • 1225-8598(pISSN)
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  • 2288-3371(eISSN)
한국어류학회 (The Ichthyological Society of Korea)
권호 표지

한국 남해에서 출현한 돌돔 (Oplegnathus fasciatus)과 강담돔 (Oplegnathus punctatus) 사이의 자연교잡종

Occurrence of Natural Hybrid between Oplegnathus fasciatus and Oplegnathus punctatus from the South Sea of Korea

  • 권혁준 (부경대학교 자원생물학과) ;
  • 김진구 (부경대학교 자원생물학과)
  • Kwun, Hyuck-Joon (Department of Marine Biology, Pukyong National University) ;
  • Kim, Jin-Koo (Department of Marine Biology, Pukyong National University)
  • 투고 : 2010.08.05
  • 심사 : 2010.09.15
  • 발행 : 2010.09.30
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초록

2008년 8월 경남 통영에서 강담돔과 돌돔의 자연교잡종 1개체가 발견되어 형태 및 유전적 특정을 강담돔 및 돌돔과 비교하였다. 형태적으로 본 총은 체측에 선명한 검은색 둥근 점을 많이 가지며 동시에 4개의 연한 가로줄을 가져 강담돔과 비슷하였다. 강담돔 및 돌돔의 계수 계측형질이 거의 일치하였으나, 체장에 대한 배지느러미길이 비에서 자연교잡종(26.7%)은 돌돔(17.2~23.6%)보다 강담돔(26.4%)에 더욱 가까웠다. 미토콘드리아 DNA 16S rRNA 510 bp를 이용한 분자분석에서 자연교잡종은 형태결과와는 달리 강담돔(d=0.020)보다 돌돔(d=0.000~0.010)에 더욱 가까웠다. 본 연구결과를 통해 경남 통영에서 발견된 자연교잡종은 암컷 돌돔과 수컷 강담돔 사이에서 태어난 것으로 추정된다.

One specimen of a natural hybrid of an Oplegnathus (Oplegnathus fasciatus $\times$ Oplegnathus punctatus) was found in Tongyeong, Korea in August 2008. We, herein, describe its morphological and genetic characteristics and compare them with those of O. fasciatus and O. punctatus. In morphology, the hybrid showed many distinctive black rounded blotches on body sides and four faint vertical bars, being in those features similar to O. punctatus. Although the counts and measurements of the hybrid mostly overlapped between O. fasciatus and O. punctatus, the Oplegnathus hybrid resembled O. punctatus in the ratio of pelvic-fin length in standard length: Oplegnathus hybrid (26.7%) was closer to O. punctatus (26.4%) than to O. fasciatus (17.2~23.6%). In genetics, as a result of analysis of 510 base pair sequences of mitochondrial DNA 16S rRNA, the hybrid was closer to O. fasciatus (d=0.000~0.010) than to O. punctatus (d=0.020). Our results suggest that the natural hybridization represented by the subject specimen occurred between an O. fasciatus female and an O. punctatus male.

키워드

참고문헌

  1. Brykov, Vl.A. and A.V. Podlesnykh. 2001. Comparative study of mitochondrial DNA in two greenling species (Hexagrammidae:Pisces) and their hybrids from Peter the Great Bay (Sea of Japan). Anim. Genet., 12: 1663-1666.
  2. Chae, B.S. and H.J. Yang. 1990. Natural hybrids between Pungitius sinensis sinensis and P. sinensis kaibarae. Korean J. Ichthyol., 2: 88-94. (in Korean)
  3. Grant, P.R. and B.R. Grant. 1992. Hybridization of bird species. Science, 256: 193-197. https://doi.org/10.1126/science.256.5054.193
  4. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser., 41: 95-98.
  5. Harada, T., H. Kumai and O. Murata. 1986. Artificial hybrids between Japanese parrot fish and spotted parrot fish. Bull. Jpn. Soc. Sci. Fish., 52: 613-621. https://doi.org/10.2331/suisan.52.613
  6. Hatooka, K. 2002. Oplegnathidae. In: Nakabo, T. (ed.), Fishes of Japan with pictorial keys to the species. English ed. Tokai Univ. Press, Tokyo, p.956.
  7. Hubbs, C.L. and K.F. Lagler. 2004. Fishes of the Great Lakes Region. Revised ed. Michigan Univ. Press., Ann Arbor., 332pp.
  8. Hwang, Y.J., M.S. Ra and C.G. Choi. 1995. A natural hybrid between spinous loach, Cobitis longicorpus and cyprinid loach Misgurunus anguilicaudatus (Pisces, Cobitidae). Korean J. Ichthyol., 7: 203-207. (in Korean)
  9. Ivanova, N.V., T.S. Zemlak, R.H. Hanner and P.D.N. Hebert. 2007. Universal primer cocktails for fish DNA barcoding. Mol. Ecol. Notes, 7: 544-548. https://doi.org/10.1111/j.1471-8286.2007.01748.x
  10. Kamei, M. and H. Takama. 1981. Natural hybrid of knifejaw-fish between Oplegnathus fasciatus and O. punctatus found in Sagami Bay. Bull. Kanagawa Pref. Fish. Exp. Stn., 10: 15-17.
  11. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated book of Korean fishes. Kyo-Hak Publishing Co., Seoul, 615pp. (in Korean)
  12. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120. https://doi.org/10.1007/BF01731581
  13. Lee, I.R., H. Yang, J.H. Kim, K.Y. Kim and I.C. Bang. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach lksookimia longicorpa by analyzing mitochondrial cor and Nuclear RAGI sequences. Korean J. Ichthyol., 21: 287-290. (in Korean)
  14. Nelson, J.S. 2006. Fishes of the world. 4th ed. John Wiley and Sons, Inc., Hoboken, New Jersey, 601pp.
  15. Shimada, Y., K. Nokubi, S. Yamamoto, O. Murata and H. Kumai. 2009. Reproduction between Oplegnathus fasciatus and O. punctatus, and fertility of their interspecies, Fish. Sci., 75: 521-523. https://doi.org/10.1007/s12562-008-0029-1
  16. Shioya, T., K Nishimura and K. Yoshida. 1973. Probable hybrids of the Oplegnathus juveniles found in Oshima waters. Japanese J. Ichthyol., 20: 47-49.
  17. Sueyoshi, M., H. Kohtsuka and S. Kai. 2009. The natural hybrid fish between Oplegnathus fasciatus and O. punctatus caught in coastal waters of Nagasaki district, Nagasaki Prefecture, Japan. Trans. Nagasaki BioI. Soc., 65: 57-60.
  18. Tamura, K., J. Dudley, M. Nei and S. Kumar. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Evol., 24: 1596-1599. https://doi.org/10.1093/molbev/msm092
  19. Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res., 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  20. Yun, Y.E., I.R. Lee, S.Y. Park, E.J. Kang, E.O. Kim, S.K. Yang, Y.K Nam and I.C. Bang. 2009. Genetic identification of hybrids between Rhodeus uyekii and R. notatus by sequence analysis of RAG-1 gene. J. Aquaculture, 22: 79-82.