Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of BTS and Androhep during Storage Times on the Kinematics and Capacitation Status in Liquid Boar Semen

BTS와 Androhep이 보존 기간 동안 액상 정액의 운동역학 및 수정능 획득에 미치는 영향

  • Kim, Yun-Hee (Department of Animal Science and Technology, Chung-Ang University) ;
  • Park, Yoo-Jin (Department of Animal Science and Technology, Chung-Ang University) ;
  • Yoon, Sung-Jae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kwon, Woo-Sung (Department of Animal Science and Technology, Chung-Ang University) ;
  • Kim, Sang-Hyun (Darby Pig AI Center) ;
  • Pang, Myung-Geol (Department of Animal Science and Technology, Chung-Ang University)
  • 김연희 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 박유진 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 윤성재 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 권우성 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 김상현 ((주)다비AI센터) ;
  • 방명걸 (중앙대학교 산업과학대학 동물자원과학과)
  • Received : 2010.09.13
  • Accepted : 2010.09.17
  • Published : 2010.09.30
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Abstract

The objective of this study was to investigate the effect of storage time on fresh boar semen in Androhep and Beltsville Thawing Solution (BTS). Boar semen samples extended in each extender were stored at $17^{\circ}C$ up to 4 days. Sperm motility kinematics was evaluated by computer assisted sperm analyzer (CASA) and capacitation status by chlortetracycline (CTC)/Hoechst 33258 staining. Sperm motility (%) was not decreased during storage in BTS and Androhep. No significant difference between extenders was observed. Only significant differences in kinematic parameters on linearity during storage were found. The percentage of dead sperm significantly decreased during storage (p<0.05). Also the percentage of noncapacitated, capacitated, and acrosome-reacted sperm significantly modified during storage (p<0.05). However, there was no significant difference between extenders except proportion of capacitated sperm. This finding supported that modification in these parameters was not significantly different between extenders during this short-term storage. Our finding strongly indicated that both Androhep and BIS maintained favorable conditions for motility, motility kinematics, and capacitation status during short-term storage. Despite modifications in some parameters were apparent during sperm storage in extenders, these may not affect the fertilizing capacity of boar semen.

Keywords

References

  1. Adeoya-Osiguwa SA, Fraser LR (2005): Cathine and norephedrine, both phenylpropanolamines, accelerate capacitation and then inhibit spontaneous acrosome loss. Hum Reprod 20:198-207.
  2. Aitken RJ, Best FS, Richardson DW, Djahanbakhch O, Mortimer D, Templeton AA, Lees MM (1982): An analysis of sperm function in cases of unexplained infertility: Conventional criteria, movement characteristics and fertilizing capacity. Fertil Steril 38:212-221.
  3. Bedu-Addo K, Lefievre L, Moseley FLC, Barratt CL-RB, Publicover SJ (2005): Bicarbonate and bovine serum albumin reversibly 'switch' capacitation-induced events in human spermatozoa. Mol Hum Reprod 11: 683-691. https://doi.org/10.1093/molehr/gah226
  4. Dube C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL (2004): Boar sperm storage capacity of BTS and androhep plus: viability, motility, capacitation, and tyrosine phosphorylation. Theriogenology 62:874-886. https://doi.org/10.1016/j.theriogenology.2003.12.006
  5. Estienne MJ, Knight JW, Beal WE (1989): Long-term liquid storage of porcine spermatozoa separated using a discontinuous bovine serum albumin gradient. J Anim Sci 67:1497-1502.
  6. Estienne MJ, Harper AF, Day JL (2007): Characteristics of sperm motility in boar semen diluted in different extenders and stored for seven days at $18{^{\circ}C}$. Reprod BioI 7:221-231.
  7. Evenson DP, Thompson L, Jost L (1994): Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility. Theriogenology 41:637-651. https://doi.org/10.1016/0093-691X(94)90174-H
  8. Fraser LR,. Abeydeera LR, Niwa K (1995): $Ca^{2+}$-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol Reprod Dev 40:233-241. https://doi.org/10.1002/mrd.1080400213
  9. Gadea J, Matas C, Lucas X (1998): Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay. Anim Reprod Sci 54:95-108. https://doi.org/10.1016/S0378-4320(98)00144-4
  10. Huo LJ, Ma XH, Yang ZM (2002): Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology 58:1349-1360. https://doi.org/10.1016/S0093-691X(02)00953-6
  11. Johnson LA, Weitze KF, Fiser P, Maxwell WMC (2000): Storage of boar semen. Anim Reprod Sci 62:143-172. https://doi.org/10.1016/S0378-4320(00)00157-3
  12. Kommisrud E, Paulenz H, Sehested E, Greule IS (2002): Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for 5 days. Acta Veterinaria Scandinavica 43:49-55. https://doi.org/10.1186/1751-0147-43-49
  13. Park YJ, Song WH, Kim YH, Mohamed EA, Oh SA, Pang MG (2008): Effect of storage times on the kinematics and capacitation status in liquid boar semen. Reprod Dev Biol 32:59-64.
  14. Sutkeviciene N, Andersson M (2005): Assessment of boar semen quality in relation to fertility with special reference to methanol stress. Theriogenology 63:739-747. https://doi.org/10.1016/j.theriogenology.2004.04.006
  15. Vishwanath R (2003): Artificial insemination: the state of the art. Theriogenology 59:571-584. https://doi.org/10.1016/S0093-691X(02)01241-4
  16. Waberski D, Magnus F, Mendonca Ferreira F, Petrunkina AM, Weitze KF, Topfer-Petersen E (2005): Importance of sperm-binding assays for fertility prognosis of porcine spermatozoa. Theriogenology 63:470-484. https://doi.org/10.1016/j.theriogenology.2004.09.025
  17. Wang WH, Abeydeera LR, Fraser LR, Niwa K (1995): Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen-thawed ejaculated boar spermatozoa incubated in a proteinfree chemically defined medium. J Reprod Fertil 104:305-313. https://doi.org/10.1530/jrf.0.1040305
  18. Watson P (1990): Artificial insemination and the preservation of semen. In: Lamming G (ed). Marshall's Physiology of Reproduction. Churchill livingstone, Edinburgh, UK, pp 747-869.
  19. Lee SH, Cheong HT, Yang BK, Park CK (2005): Development of semen extenders by assessment of sperm viability in miniature-pig semen. Reprod Dev Biol 29:247-252.
  20. Yanagimachi R (1994): Mammalian fertilization. In Knobil E and Neill JD (eds). The Physiology of Reproduction 2nd ed. Raven Press. New York, USA, pp 189-317.