Reproductive and Developmental Biology

  • 제34권3호
  • /
  • Pages.235-240
  • /
  • 2010
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

한우에서 임신 초기 발현 차이 혈장 단백질의 단백질체학적 분석

Proteomic Analysis of Differentially Expressed Plasma Proteins during Early Pregnancy in Hanwoo

  • 김평희 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 권대진 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 양병철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 임기순 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 민관식 (한경대학교 생물환경정보통신전문대학원) ;
  • 윤종택 (한경대학교 생물환경정보통신전문대학원) ;
  • 진동일 (충남대학교 축산학과) ;
  • 박수봉 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황성수 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • 투고 : 2010.09.10
  • 심사 : 2010.09.17
  • 발행 : 2010.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups; Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo.

키워드

참고문헌

  1. Bae SH, Yang BC, Joo SC, Min KS, Yoon JT, Jin DI, Kim TH, Seong HH, Hwang S (2008): Expression levels of plasma angiogenic factors during early pregnancy in Hanwoo. Reprod Dev Biol 32:263-267.
  2. Balakrishnan SD, Anuradha CV (1998): Exercise, depletion of antioxidants and antioxidant manipulation. Cell Biochemistry Function 16:269-275. https://doi.org/10.1002/(SICI)1099-0844(1998120)16:4<269::AID-CBF797>3.0.CO;2-B
  3. Buhimschi CA, Bhandari V, Hamar BD, Bahtiyar MO, Zhao G, Sfakianaki AK, Pettker CM, Magloire L, Funai E, Norwitz ER (2007): Proteomic profiling of the amniotic fluid to detect inflammation, infection, and neonatal sepsis. PLos Med 4(1): e18. 84-94.
  4. Burtist CA, Ashwood ER (1994): Tietz Textbook of Cinical Chemistry. 2nd ed. WB Saunders Company. Philadelphia, pp 688-691.
  5. Gan KN, Smolen A, Eckerson HW, La Du BN (1991): Purification of human serum paraoxonase/arylesterase. Evidence for one esterase catalyzing both activities. Drug Metab Dispos 19:100-106.
  6. Govorukhina NI, Keizer-Gunnink A, van der Zee AG, de Jong S, de Bruijn HW, Bischoff R (2003): Sample preparation of human serum for the analysis of tumor markers. Comparison of different approaches for albumin and gamma-globulin depletion. J Chromatogr A 1009:171-178. https://doi.org/10.1016/S0021-9673(03)00921-X
  7. Hoffer-Goeres KA, Batchelder CA, Bertolini M, Moyer AL, Famula TR, Anderson GB (2007): Angiogenesis in day-30 bovine pregnancies derived from nuclear transfer. Cloning and Stem Cells 9:595-607. https://doi.org/10.1089/clo.2007.0019
  8. Hwang S, Chang YM, Ko YG, Yang Be, Min KS, Yoon JT, Nho WG, Kim CK, Seong HH (2009): Delayed parturition in cloned calves associated with persistently elevated placentomal TGF-$\beta$1 expression. Anim Reprod Sci 115:290-295. https://doi.org/10.1016/j.anireprosci.2008.11.013
  9. Jarkovska K, Martinkova J, Liskova L, Halada P, Moos J, Rezabek K, Gadher SJ, Kovarova H (2010): Proteome mining of human follicular fluid reveals a crucial role of complement cascade and key biological pathways in women undergoing in vitro Fertilization. J Proteome Res 9:1289-1301. https://doi.org/10.1021/pr900802u
  10. Josse D, Lockridge O, Xie W, Bartels CF, Schopfer LM, Masson P (2000): The active site of human paraoxonase (PON1). J Appl Toxiocol 21:57-11.
  11. King GJ, Atkinson BA, Robertson HA (1979): Development of the bovine placentome during the second month of gestation. J Reprod Fertil 55:173-180. https://doi.org/10.1530/jrf.0.0550173
  12. La Du BN (1996): Structural and functional diversity of paraoxonase. Nat Mol 2:1186-1187. https://doi.org/10.1038/nm1196-1186
  13. Mackness MI, Mackness B, Durrington PA, Connelly PW, Hegele RA (1996): Paraoxonase: biochemistry, genetics and relationship lipoproteins. Curr Opin Lipidol 7:69-76. https://doi.org/10.1097/00041433-199604000-00004
  14. Nguyen S, Sok D (2003): Oxidative inactivation of paraoxonase 1, an antioxidant protein and its effect on antioxidant action. Free Radical Res 37:1319-1330.
  15. Oakley BR, Kirsch DR, Morris NR (1980): A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105:361-363. https://doi.org/10.1016/0003-2697(80)90470-4
  16. Riding GA, Jones A, Holland MK (2008): Proteomic analysis of bovine conceptus fluids during early pregnancy. Proteomics 8:160-177. https://doi.org/10.1002/pmic.200700465
  17. Roche JF, Mackey D, Diskin MD (2000): Reproductive management of postpartum cows. Anim Reprod Sci 60-61:703-712. https://doi.org/10.1016/S0378-4320(00)00107-X
  18. Spitzer D, Murach KF, Lottspeich F, Staudach A, Illmensee K (1996): Different protein patterns derived from follicular fluid of mature and immature human follicles. Hum Reprod 11:798-807. https://doi.org/10.1093/oxfordjournals.humrep.a019257
  19. Steel LF, Trotter MG, Nakajima PZ, Mattu TS, Gonye G, Block T (2003): Efficient and specific removal of albumin from human serum samples. Mol Cell Proteomics 2:262-270.
  20. Taponen J, Katila T, Rodriguez-Martinez H (1999): Induction of ovulation with gonadotropin releasing hormone during proestrus in cattle: influence on subsequent follicular growth and luteal function. Anim Reprod Sci 55:91-105. https://doi.org/10.1016/S0378-4320(99)00011-1
  21. Turk R, Juretic D, Geres D, Turk N, Rekic B, Simenon-rudolf V, Svetina A (2004): Serum paraoxonase activity nad lipid parameters in the earl postpartum period of dairy cows. Res Vet Sci 76:57-61. https://doi.org/10.1016/j.rvsc.2003.08.001
  22. Wang YY, Cheng P, Chan DW (2003): A simple affinity spin tube filter method for removing high-abundant common proteins or enriching low-abundant biomarkers for serum proteomic analysis. Proteomics 3:243-248. https://doi.org/10.1002/pmic.200390036
  23. Wiese AG, Pacifici RE, Davies KJA (1995): Transient adaptation to oxidative stress in mammalian cells. Archives of Biochemistry and Biophysics 318:231-240. https://doi.org/10.1006/abbi.1995.1225
  24. 김영훈, 고진철, 오창언, 강승률, 양보석, 오성종, 김창능, 송중용, 김일화 (2006): CIDR를 이용한 제주 한우 및 흑우의 체내 수정란 생산과 이식. 한국수정란이식학회지 21:191-198.
  25. 김평희, 배성훈, 오석두, 고응규, 양병철, 김명직, 진동일, 성환후, 황성수 (2008): 한우의 임신 초기 혈장 단백질 발현 양상 분석. 한국수정란이식학회지 23:25-30.
  26. 성환후, 양병철, 황성수, 박성재, 고응규, 노환국, 양보석 (2006): Anti-inhibin serum 및 bovine follicular fluid 처리가 한우 난소의 난포 발달과 혈중 호르몬 변화에 미치는 영향. 한국수정란이식학회지 21:315-322.
  27. 이귀녕, 이종순 (1996): 임상병리화일. 의학문화사. 서울, pp 192-194.
  28. 황성수, 최선호, 장유민, 고응규, 양병철, 임기순, 민관식, 성환후 (2008): 체세포 복제란 이식 한우의 분만 전 혈장 Progesterone과 Estradial-17$\beta$ 농도 변화. 한국동물번식학회지 32:199-203.