Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Whitening and Anti-wrinkle Effects of Prunus persica Flos

도화(桃花, Prunus persica Flos)의 미백 및 주름개선 효과

  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University)
  • 이진영 (호서대학교 한방화장품과학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2010.06.21
  • Accepted : 2010.07.21
  • Published : 2010.09.30
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Abstract

The solvent extracts of Prunus persica Flos were investigated for the activities of whitening and anti-wrinkle effects to apply as a functional ingredient for cosmetic products. The tyrosinase inhibitory effect, which is related to skin-whitening, was 54.0, 58.3% in P. persica Flos (PPW, PPE) at 1,000 ppm. In addition, the ethanol extract of P. persica Flos (PPE) showed a potent tyrosinase inhibitory activity in the test using melanoma cell lines resulting in 40.0% inhibition at 100ppm. Furthermore, the aqueous acetone extract from the flower of P. persica Flos was found to inhibit elastase, which was more effective than ascorbic acid at 1,000 ppm. The inhibition of melanin synthesis by P. persica Flos extract (PPE) was about 56.5% at 100 ppm concentration. When compared to other extraction methods, the ethanol extract showed more potent whitening activity. For anti-wrinkle effect, the elastase inhibition activity of P. persica Flos extract (PPA) was 57.0% and higher than that of ascorbic acid at 1,000 ppm. The collagenase inhibition activity of P. persica Flos extract (PPA) was about 48.0% at 1,000 ppm. Collagen synthesis in fibroblast cell by P. persica Flos extracts (PPA) was about 41.0% at 100 ppm and its acetone extract was the best showing antiwrinkle activities. All these findings suggested that P. persica Flos has a great potential as a cosmeceutical ingredient with a whitening and anti-wrinkle effect.

도화를 기능성 화장품 소재로 활용하기 위하여 미백 및 주름개선 효과를 검증한 후 화장품 소재로서의 가능성을 검증하였다. 미백효과를 확인하기 위하여 tyrosinase 저해활성을 측정한 결과 도화 열수 및 에탄올 추출물 1,000ppm에서 각각 54.0, 58.3%의 저해활성을 나타내었으며, 멜라노마 세포에서의 tyrosinase 저해활성을 측정한 결과 도화 에탄올 추출물 100ppm에서 40.0%의 저해활성을 나타내였다. 또한 멜라노마 세포에서의 멜라닌 생합성 저해율을 확인한 결과 도화 에탄올 추출물 100ppm에서 56.5%의 효과를 나타내어 도화 에탄올 추출물의 미백효과가 가장 우수하였다. 주름개선 효과 측정으로 elastase 저해활성을 측정한 결과 도화 아세톤 추출물 1,000ppm에서 57.0%의 저해활성을 나타내었으며, collagenase 저해활성을 측정한 결과 도화 아세톤 1,000ppm에서 48.0%의 저해활성을 나타내었다. 또한 섬유아세포를 이용한 collagen 생합성량을 측정한 결과 도화 아세톤 추출물 100ppm에서 41.0%의 효과를 나타내어 도화 아세톤 추출물의 주름개선 효과가 가장 우수하였다. 이상의 결과로 미루어 보아 도화 추출물의 미백 및 주름개선 효과가 있음을 확인할 수 있었고, 기능성 화장품 소재로서의 가능성을 확인할 수 있었다.

Keywords

References

  1. An BJ, Lee CE, Son JR, Lee JY, Choi GR, and Park TS (2005) Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rlwdodendron mucronulatum T. J Korean Soc Appl BioI Chem 48, 280-284.
  2. Barrantes E, and Guinea M (2003) Inhibition of collagenase and metallo- proteinases by aloins and aloe gel. Life Sci 72, 843-850. https://doi.org/10.1016/S0024-3205(02)02308-1
  3. Bernstein EF, Chen YQ, Tamai K. Shepley KJ, Resnik KS, Zhang H, Tuan R, Mauviel A, and Uitto J (1994) Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol 103, 182-186. https://doi.org/10.1111/1523-1747.ep12392693
  4. Cannell RJP, Kellan SJ, Owsianks AM, and Walker JM (1988) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54, 10-14. https://doi.org/10.1055/s-2006-962319
  5. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, and Mitchell JB (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47, 936-942.
  6. Choi SY, Kang NJ, and Kim HC (2006) Inhibitory effect of root extracts on melanin biosynthesis in Rodgersia podophylla A. Gray. Korean J Medicinal Crop Sci 14, 27-30.
  7. Choi WY, Chun HJ, Lee JH, and Baek SH (2003) Effect of methanol extract from Cornis fructus on melanogenesis. Kor J Pharmacogn 34, 70-74.
  8. Chun HJ, Choi WH, Baek SH, and Woo WH (2002) Effect of Quercetin on melanogenesis in melan-a melanocyte cells. Kor J Pharmacogn 33, 245-251.
  9. Demina NS, and Lysenko SV (1996) Collagenolytic enzymes synthesized by microorganisms. Mikrobiologiia 65, 293-304.
  10. El-Domyati M, Attia S, Saleh F, Brown D, Birk DE, Gasparro F, Ahmad H, and Uitto J (2001) Intrinsic aging vs. photoaging: a comparative histo- pathological, inununohistochemical, and ultrastructural study of skin. Exp Dermatol 11, 398-405.
  11. Giacomoni PU, and Rein G (2001) Factors of skin ageing share common mechanisms. Biogerontology 2, 219-229. https://doi.org/10.1023/A:1013222629919
  12. Gilchrest BA (1990) Skin aging and photoaging. Dermatol Nurs 2, 79-82.
  13. Grant NH, and Album HE (1959) Studies on the collagenases of Clostridium histolyticum. Arch Biochem Biophys 82, 245-255. https://doi.org/10.1016/0003-9861(59)90120-1
  14. Han J, Kim HJ, Lee SS, and Lee IS (1999) Inhibitive effects of meju extracts made with a single inoculum of the fungi isolated from the traditional meju on the human leukemia cell line. Korean J Mycology 27, 312-317.
  15. Hearing VJ Jr (1987) Mammalian monophenol monooxygenase (tyrosina se): purification, properties, and reactions catalyzed. Methods Enzymol 142, 154-165. https://doi.org/10.1016/S0076-6879(87)42024-7
  16. Hearing VJ, and Jimenez M (1987) Mammalian tyrosinase the critical regu- latory control point in melanocyte pigmentation. Int J Biochem 19, 1141. https://doi.org/10.1016/0020-711X(87)90095-4
  17. Heo J (1994) Donguibogam. Daejoongseokwan Press, pp. 173, 301, 1386. Seoul, Korea.
  18. Hosoi J. Abe E, Suda T, and Kuroki T (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 45,1474-1478.
  19. Hur JM, Lee JH, Choi JW, Hwang GW, Chung SK. Kim MS, and Park JC (1998) Effect of methanol extract and kaempferol glycosides from armoracia rusticana on the formation of lipid peroxide in bromo benzene-treated rats in vitro. Kor J Pharmacogn 29, 231-236.
  20. Jung SW, Lee NK. Kim SJ, and Han DS (1995). Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27, 891-896.
  21. Kim IH etc (1991) New medicines botany. pp. 279. Hakchangsa Press, Seoul, Korea.
  22. Kim MJ, Kim JY, Choi SW, Hong IT, and Yoon KS (2004) Antiwrinkle effect of safflower(Cathamus tinctorius) seed extract. J Soc Cosmet Scientisis Korea 30, 15-22.
  23. Kim SH, Cha EJ, and Hwang YJ (2004) Studies on antitumor activity and antimicrobial activity of Coriolus Versicolor (Fr.) Qual and Ganoderma Lucidum (Fr.) Karst. Korean J Human Ecology 7, 49-58.
  24. Kwak JH, Kim YH, Chang HR, Park CW, and Han YH (2004) Inhibitory effect of gardenia fruit extracts on tyrosinase activity and melanogenesis. Korean J Biotechnol Bioeng 19, 437-440.
  25. Kwak YJ, Lee DH, Kim NM, and Lee JS (2005) Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Korean J Medicinal Crop Sci 13, 213-216.
  26. Lee HJ, Lee EO, Lee YR, Kim KH, Lee JH, Baek NI, Ra JC, and Kim SH (2004) Anti-tumor activity of Korean oldenlandiae Herba and Radix. Kor J Pharmacogn 35, 110-115.
  27. Lee NH, Yang HC, Bu HJ, Jung DS, Lee SJ, and Riu KZ (2001) Screening of the tyrosinase inhibition and hyaluronidase inhibition activities and radical scavenging effects using plants in Cheju. Kor J Pharmacogn 32, 175-180.
  28. Lee SS, Mun SH, Lee HJ, Choe DH, and Jo MH (2004) Cholesterol inhibitory activities of kaempferol and quercetin isolated from Allium victorialis var. platypbyllum. Mokchae Konghak 32, 17-27.
  29. Lee TW, Kim SN, Jee UK, and Hwang SJ (2004) Anti-wrinkle effect of pressure sensitive adhesive hydrogel patches containing Ulmi cortex extract. J Kor Pharm Sci 34, 193-199.
  30. Li G, Ju HK, Chang HW, Jahng YD, Lee SH, and Son JK (2003) Melanin biosynthesis inhibitors from the bark of Machi/us thunbergii. Biol Pharm Bull 26, 1039-1041. https://doi.org/10.1248/bpb.26.1039
  31. Mauviel A, Halcin C, Vasiloudes P, Parks WC, Kurkinen M, and Uitto J (1994) Uncoordinate regulation of collagenase, stromelysin, and tissue inhibitor of metalloproteinases genes by prostaglandin E2: selective enhancement of collagenase gene expression in human dermal fibroblasts in culture. J cell Biochem 54, 465-472. https://doi.org/10.1002/jcb.240540413
  32. Park SK, Kim SN, Lee JC, Kim HS, Kim YJ, Lee Bo, and Chang IS (2004) Anti-aging effect on skin with Jaeum-Dan(JED). Kor J Herbology 19, 67-76.
  33. Pentland AP, Shapiro SD, and Welgus HG (1995) Agonist-induced expression of tissue inhibitor of metalloproteinases and metalloproteinases by human macrophages is regulated by endogenous prostaglandin E2 synthesis. J Invest Dermatol 104, 52-57. https://doi.org/10.1111/1523-1747.ep12613488
  34. Seo G (2005) Koryeodokyung. Seohaemoonjib press, pp. 276. Gyeonggi-do, Korea.
  35. Seo MH, and Lee YM (2000) The we tree to fmd easily(3). pp. 146-147. hyeonamsa Press, Seoul, Korea.
  36. Tsuji N, Moriwaki S, Suzuki Y, Takema Y, and Imokawa G (2001) The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74, 283-290. https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  37. Wunsch E, and Heindrich HG (1963) Zur quantitativen bestimmung der collagenase. Hoppe-Seyler's Physiol Chem 333, 149-151. https://doi.org/10.1515/bchm2.1963.333.1.149
  38. Yagi A, Kanbara T, and Morinobu N (1986) The effect of tyrosinase inhibition for aloe. Planta Medica 3981, 517-519.

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