Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of Taurine Supplementation on Performance, Egg Quality, Blood Parameter and Liver Lipid and Lipid Peroxidation Levels of Laying Hens Fed High Fat Diet

고지방 사료에 타우린 첨가가 산란계의 생산성, 계란 품질, 혈액 성상, 간 조직의 지질 및 지질과산화물 수준에 미치는 영향

  • Shim, Kwan-Seob (Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Park, Garng-Hee (Department of Animal Biotechnology, Chonbuk National University) ;
  • Na, Chong-Sam (Department of Animal Biotechnology, Chonbuk National University) ;
  • Ji, Joong-Ryong (Paek Kwang C&S Co.) ;
  • Choe, Ho-Sung (Department of Animal Biotechnology, Chonbuk National University)
  • 심관섭 (전북대학교 농업과학기술연구소) ;
  • 박강희 (전북대학교 동물생명공학과) ;
  • 나종삼 (전북대학교 동물생명공학과) ;
  • 지중룡 ((주)백광 C&S) ;
  • 최호성 (전북대학교 동물생명공학과)
  • Received : 2010.10.27
  • Accepted : 2010.12.07
  • Published : 2010.12.31
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Abstract

The current study was conducted to estimate the effect of dietary taurine on performance, egg quality, blood parameter, liver lipids and lipid peroxidation level of laying hens fed high fat diet. Two hundred eighty laying hens, forty five weeks of age, were allocated to four treatment groups with seven replicates of 10 each per treatment for 4 weeks. Experimental diets were assigned to each of the four groups: control diet (CON), CON with 0.5% taurine (CT), CON with 5% soybean oil (HF), and CON with 5% soybean oil and 0.5% taurine (HFT). Egg weight was significantly lower in the control than the CT (P<0.05). However, the HFT was similar to the level compared to both control and HF. Serum concentrations of total cholesterol, triglyceride and glutamic oxaloacetic transaminase (GOT) were significantly decreased by about 30%, 36% and 20%, respectively in the HFT compared to the HF (P<0.05). In the liver tissue, triglyceride concentration tended to be lower by about 28% in the HFT compared to the HF (P<0.05). The hepatic lipid peroxidation level was significantly decreased by about 25% in the HFT compared to the HF (P<0.05). These results indicated that taurine supplementation improved the serum total cholesterol, triglyceride concentration, and decreased hepatic lipid peroxidation level without affecting performance in laying hens fed high fat diet.

본 실험은 타우린이 고지방 사료를 급여한 산란계의 생산성, 계란 품질, 혈액 성상, 간 조직의 지질 및 지질과산화물 수준에 미치는 영향을 평가하였다. 45주령 Hy-Line 갈색계 280수를 4개의 처리구로 구분하여 처리구당 7반복, 반복당 10수씩 배치하여 4주간 실시하였다. 실험 처리구는 기초 사료를 대조구로 하고, 기초 사료+0.5% 타우린(CT), 기초 사료+5% 대두유(HF) 그리고 기초 사료+5% 대두유+0.5% 타우린(HFT)로 구분하였다. 난중은 CT가 대조구보다 유의적으로 낮았으나(P<0.05), HFT는 대조구 그리고 HF와 비교하여 비슷한 수준이었다. 혈청 중 총 콜레스테롤, 중성지방 그리고 GOT 농도에서 HFT는 HF보다 유의적으로 각각 약 30%, 36% 그리고 20% 감소하였다(P<0.05). 간 조직에서 중성지방 농도는 HFT보다 HF보다 약 28% 낮은 경향이었으며, 지질과 산화물 농도는 HFT가 HF보다 유의적으로 약 25% 감소하였다(P<0.05). 따라서 본 연구 결과, 고지방 사료에 타우린의 급여는 산란계의 생산성에 영향을 미치지 않으면서 혈청 중 총 콜레스테롤과 중성지방 농도를 개선하였으며, 간 조직의 지질과산화물 농도를 감소시키는 것으로 평가되었다.

Keywords

References

  1. Balkan J, Dogru-Abbasoglu S, Kanbagli O, Cevikbas U, Aykac-Toker G, Uysal M 2001 Taurine has a protective effect against thioacetamide-induced liver cirrhosis by decreasing oxidative stress. Hum Exp Toxicol 20:251-254. https://doi.org/10.1191/096032701678227758
  2. Balkan J, Kanbagli O, Aykac-Toker G, Uysal M 2002 Taurine treatment reduces hepatic lipids and oxidative stress in chronically ethanol-treated rats. Biol Pharm Bull 25:1231-1233. https://doi.org/10.1248/bpb.25.1231
  3. Balkan J, Kanbagli O, Hatipoglu A, Kucuk M, Cevikbas U, Aykac-Toker G, Uysal M 2002 Improving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on ahigh-cholesterol diet. Biosci Biotechnol Biochem 66:1755-1758. https://doi.org/10.1271/bbb.66.1755
  4. Bligh EG, Dyer WJ 1959 A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917. https://doi.org/10.1139/o59-099
  5. Bohnsack CR, Harms RH, Merkel WD, Russell GB 2002 Performance of commercial layers when fed diets with four contents of corn oil or poultry fat. J Appl Poult Res 11: 68-76. https://doi.org/10.1093/japr/11.1.68
  6. Bryant MG, Wu G, Roland DA Sr 2005 Optimizing dietary energy for profits and performance of two strains of White Leghorns. Page 23 in: Inernational Poultry Scientific Forum Abstracts, Atlanta, GA, USA.
  7. Cantafora A, Blotta I, Rossi SS, Hofmann AF, Sturman JA 1991 Dietary taurine content changes liver lipids in cats. J Nutr 121:1522-1528.
  8. Cantafora A, Mantovani A, Masella R, Mechelli L, Alvaro D 1986 Effect of taurine administration on liver lipids in guinea pig. Experientia Apr 15:407-408.
  9. Cho BHS 1983 Improved enzymatic determination of total cholesterol in tissues. Clin Chem 29:166-168.
  10. Davies KJ 1986 Intracellular proteolytic systems may function as secondary antioxidant defenses: an hypothesis. J Free Radic Biol Med 2:155-173. https://doi.org/10.1016/S0748-5514(86)80066-6
  11. Ding WG, Tooyama I, Kimura H, Kuriyama K, Ochi J 1993 Distribution of taurine-like immunoreactivity in the mouse liver during ontogeny and after carbon tetrachloride or phenobarbital intoxication. Histochem J 25:376-383. https://doi.org/10.1007/BF00159502
  12. Folch J, Lees M, Sloane-Stanley GH 1957 Simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497-509.
  13. Gaull GE, Pasantes-Morales H, Wright CE 1985 Taurine in human nutrition: overview. Prog Clin Biol Res 179:3-21.
  14. Grobas S, Mendez J, De Blas C, Mateos GG 1999 Laying hen productivity as affected by energy, supplemental fat, and linoleic acid concentration of the diet. Poultry Sci 78:1542-1551. https://doi.org/10.1093/ps/78.11.1542
  15. Grobas S, Mendez J, Lazaro R, de Blas C, Mateo GG 2001 Influence of source and percentage of fat added to diet on performance and fatty acid composition of egg yolks of two strains of laying hens. Poultry Sci 80:1171-1179. https://doi.org/10.1093/ps/80.8.1171
  16. Hansen RJ, Walzem RL 1993 Avian fatty liver hemorrhagic syndrome: A comparative review. Adv Vet Sci Comp Med 37:451-468.
  17. Harms RH, Russell GB, Sloan DR 2000 Performance of four strains of commercial layers with major changes in dietary energy. J Apple Poult Res 9:535-541. https://doi.org/10.1093/japr/9.4.535
  18. Huxtable RJ 1992 Physiological actions of taurine. Physiol Rev 72:101-163.
  19. Kocak-Toker N, Giris M, Tulubas F, Uysal M, Aykac-Toker G 2005 Peroxynitrite induced decrease in $Na^{+}$, $K^{+}$-ATPase activity is restored by taurine. World J Gastroenterol 11: 3554-3557. https://doi.org/10.3748/wjg.v11.i23.3554
  20. Lee DN, Cheng YH, Chuang YS, Shive JL, Lian YM, Wei HW, Weng CF 2004 Effects of dietary taurine supplementation on growth performance, serum constituents and antibody production of broilers. Asian-Aust J Anim Sci 17:109-115. https://doi.org/10.5713/ajas.2004.109
  21. Lee SM, Lim HS, Namgung N, Lee BH, Paik IK 2010 Taurine enrichment of egg with feather meal and pyridoxine. Asian- Aust J Anim Sci 23:622-629. https://doi.org/10.5713/ajas.2010.90572
  22. Li J, Foote RH, Simkin M 1993 Development of rabbit zygotes cultured in protein-free medium with catalase, taurine, or superoxide dismutase. Biol Reprod 49:33-37. https://doi.org/10.1095/biolreprod49.1.33
  23. Machlin LJ, Bendich A 1987 Free radical tissue damage: protective role of antioxidant nutrients. FASEB J 1:441-445.
  24. Militante JD, Lombardini JB 2004 Dietary taurine supplementation: hypolipidemic and antitherogenic effects. Nutr Res 24:787-801. https://doi.org/10.1016/S0271-5317(04)00109-5
  25. Park T, Lee K 1998 Dietary taurine supplementation reduces plasma and liver cholesterol and triacylglycerol levels in rats fed a high-cholesterol or a cholesterol-free diet. Adv Exp Med Biol 442:319-325.
  26. Petty MA, Kintz J, DiFrancesco GF 1990 The effects of taurine on atherosclerosis development in cholesterol-fed rabbits. Eur J Pharmacol 180:119-127. https://doi.org/10.1016/0014-2999(90)90599-2
  27. SAS/STAT 1996 SAS User guide release 6.12 edition.
  28. Shim KS, Hwang KT, Son MW, Park GH 2006 Lipid metabolism and peroxidation in broiler chicks under chronic heat stress. Asian-Aust J Anim Sci 19:1206-1211. https://doi.org/10.5713/ajas.2006.1206
  29. Shinnburber RO, Yu TC 1958 Characterization of the red pigment formed in 2-thiobarbituric acid determination of oxidation rancibility. Food Res 23:620-629.
  30. Sohail SS, Bryant MM, Roland DA Sr 2003 Influence of dietary fat on economic returns of commercial leghorns. J Appl Poult Res 12:356-361. https://doi.org/10.1093/japr/12.3.356
  31. Squires EJ, Leeson S 1988 Aetioiogy of fatty liver syndrome in laying hens. Br Vet J 144:602-609. https://doi.org/10.1016/0007-1935(88)90031-0
  32. Summers JD, Leeson S 1993 Influence of diets varying in nutrient density on the development and reproductive performance of White Leghorn pullets. Poultry Sci 72:1500-1509. https://doi.org/10.3382/ps.0721500
  33. Trachtman H, Futterweit S, Bienkowski RS 1993 Taurine prevents glucose-induced lipid peroxidation and increased collagen production in cultured rat mesangial cells. Biochem Biophys Res Commun 191:759-765. https://doi.org/10.1006/bbrc.1993.1282
  34. Waterfield CJ, Mesquita M, Parnham P, Timbrell JA 1993 Taurine protects against the cytotoxicity of hydrazine, 1,4-naphthoquinone and carbon tetrachloride in isolated rat hepatocytes. Biochem Pharmacol 46:589-595. https://doi.org/10.1016/0006-2952(93)90542-5
  35. Wu G, Bryant MM, Voitle RA, Roland DA Sr 2005 Effects of beta-mannanase in corn-soy diets on commercial Leghorns in second-cycle hens. Poultry Sci 84:894-7. https://doi.org/10.1093/ps/84.6.894
  36. Yamazaki M, Takemasa 1998 Effects of dietary taurine on egg weight. Poultry Sci 77:1024-1026. https://doi.org/10.1093/ps/77.7.1024
  37. Yan CC, Bravo E, Cantafora A 1993 Effect of taurine levels on liver lipid metabolism: An in vivo study in the rat. Proc Soc Exp Biol Med 202:88-96. https://doi.org/10.3181/00379727-202-43516
  38. Yany Y, Xiao R, Qiu F, Li X 2002 Effect of taurine on blood and liver lipids in rats. Wei Sheng Yan Jiu 31:63-65.
  39. Yokogoshi H, Mochizuki H, Nanami K, Hida Y, Miyachi F, Oda H 1999 Dietary taurine enhances cholesterol degradation and reduces serum and liver cholesterol concentrations in rats fed a high-cholesterol diet. J Nutr 129:1705-1712.
  40. Zou SG, Wu YZ 2005 Effects of supplemental fat on performance of laying hens. Int J Poult Sci 4:988-1000.
  41. 김정학 심관섭 박강희 2002 타우린 첨가가 산란계의 난생 산성 및 난질에 미치는 영향. 한국가금학회지 29:171-176.
  42. 박강희 2002 사료내 타우린 첨가가 산란계의 지방대사에 미치는 영향. 한국가금학회지 29:95-100.
  43. 박강희 최형송 1997 타우린 첨가가 육계의 성장에 미치는 영향과 생리학적 기전. 한국동물자원과학회지 39:124-134.
  44. 박태선 2001 타우린의 생리활성과 영양학적 의의. 한국식품영양학회지 34:597-607.